FastQCFastQC Report
Fri 27 Jan 2017
SRR1917539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1917539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26943015
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT13075800.042.8281245
CTGCTTG666200.037.70482345
GTCGGAC10250.032.097691
TCGGACT10850.027.4140782
TGTTCGA14400.026.8679630
CGAGAGT69450.026.72130617
ATTGAGT76100.026.57363718
TGAGAGT230850.026.01700817
ACTCAGT239550.025.54163418
TGATAGT142700.025.28461617
TGTTAGT100750.025.02859917
GTTTAGT126900.024.99386818
TTCAGTC581150.024.83398619
TTCGAAT15150.024.79635432
ACTTAGT190500.024.67898218
TTAGAGT185150.024.63883617
AATGCCG14800.024.62411936
CGATAGT28850.024.56069817
ATTTAGT204550.024.55642918
GTTCAGT106750.024.54901118
Produced by FastQC (version 0.11.5)