FastQCFastQC Report
Thu 2 Feb 2017
Polstein2015_Rep1_trimmed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePolstein2015_Rep1_trimmed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26943015
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAC10050.010.1825691
TCGGACT10850.08.5270612
GTTCGTA4050.07.60090910
TTAGTCG4450.07.54710814
TCGTATG3500.06.59658912
ATGTTCG5150.06.3850128
CGGACTG15050.06.38024953
TACGAAC3504.456524E-105.8076511
ACAGTCG8850.05.7713714
GTAGAAC12200.05.7347089
GACTGTA17150.05.6297315
CGTATGC4055.838956E-105.35528513
TGTTCGT6150.05.3467999
CGAACAT10900.05.27300171
CTACTAA44200.05.1760289
TCTACTA44000.05.1359528
Produced by FastQC (version 0.11.5)