Script file | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/chipseq.bds |
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Program ID | chipseq.bds.20161114_172028_183 |
Start time | 2016-11-14 17:20:28 |
Run time | 03:30:28.677 |
Tasks executed | 15 |
Tasks failed | 0 |
Tasks failed names | |
Arguments* | [-title, SRR1370878_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
System* | sge |
Cpus* | -1 |
Exit value | 0 |
Thread ID | Parent | Tasks |
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thread_Root | None | chipseq.bds.20161114_172028_183/task.graphviz.report.line_97.id_23 chipseq.bds.20161114_172028_183/task.report.pdf2png.line_324.id_28 |
thread_41 | thread_Root | chipseq.bds.20161114_172028_183_parallel_41/task.align_bwa.bwa_aln_rep1.line_108.id_11 chipseq.bds.20161114_172028_183_parallel_41/task.align_bwa.bwa_sam_rep1.line_140.id_14 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bam.dedup_bam_1_rep1.line_95.id_16 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bam.markdup_bam_picard_rep1.line_348.id_17 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bam.dedup_bam_2_rep1.line_144.id_18 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bam.bam_to_tag_rep1.line_505.id_19 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bed.subsample_tag_rep1.line_45.id_20 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bed.spr_rep1.line_255.id_21 chipseq.bds.20161114_172028_183_parallel_41/task.postalign_bed.xcor_rep1.line_212.id_22 |
thread_42 | thread_Root | chipseq.bds.20161114_172028_183_parallel_42/task.postalign_bam.dedup_bam_1_ctl1.line_95.id_10 chipseq.bds.20161114_172028_183_parallel_42/task.postalign_bam.markdup_bam_picard_ctl1.line_348.id_12 chipseq.bds.20161114_172028_183_parallel_42/task.postalign_bam.dedup_bam_2_ctl1.line_144.id_13 chipseq.bds.20161114_172028_183_parallel_42/task.postalign_bam.bam_to_tag_ctl1.line_505.id_15 |
Task | Execution | Time | Dependencies | Task program, Errors, StdOut / StdErr | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 97 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 105 if [[ 0 > 0 ]]; then \ sambamba sort -t 3 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \ samtools view -F 1804 -Su /dev/stdin | \ sambamba sort -t 3 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ else \ samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam | \ sambamba sort -t 3 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ fi # SYS command. line 116 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 23713 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 110 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 113 bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.sai # SYS command. line 115 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 25025 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- [bwa_aln] 17bp reads: max_diff = 2 [bwa_aln] 38bp reads: max_diff = 3 [bwa_aln] 64bp reads: max_diff = 4 [bwa_aln] 93bp reads: max_diff = 5 [bwa_aln] 124bp reads: max_diff = 6 [bwa_aln] 157bp reads: max_diff = 7 [bwa_aln] 190bp reads: max_diff = 8 [bwa_aln] 225bp reads: max_diff = 9 [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.56 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 262144 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.02 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 524288 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.09 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 786432 sequences have been processed. [bwa_read_seq] 2.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 90.60 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 1048576 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.30 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 1310720 sequences have been processed. [bwa_read_seq] 3.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.60 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 1572864 sequences have been processed. [bwa_read_seq] 2.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.34 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 1835008 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.38 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 2097152 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.77 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 2359296 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 77.24 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 2621440 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 78.72 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 2883584 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.12 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 3145728 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.05 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 3407872 sequences have been processed. [bwa_read_seq] 2.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 90.95 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 3670016 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.71 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 3932160 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.63 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 4194304 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.13 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 4456448 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.65 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 4718592 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.75 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 4980736 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.81 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 5242880 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.42 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 5505024 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.27 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 5767168 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.07 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 6029312 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.47 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 6291456 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.10 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 6553600 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.28 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 6815744 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.01 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 7077888 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.59 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 7340032 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.05 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 7602176 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 88.76 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 7864320 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.55 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 8126464 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.02 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 8388608 sequences have been processed. [bwa_read_seq] 2.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.72 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 8650752 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.96 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 8912896 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.25 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 9175040 sequences have been processed. [bwa_read_seq] 5.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.07 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 9437184 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 89.01 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 9699328 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.05 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 9961472 sequences have been processed. [bwa_read_seq] 7.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.21 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 10223616 sequences have been processed. [bwa_read_seq] 8.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 100.61 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 10485760 sequences have been processed. [bwa_read_seq] 13.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 54.47 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 10747904 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.88 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 11010048 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.41 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 11272192 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.61 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 11534336 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.47 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 11796480 sequences have been processed. [bwa_read_seq] 6.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 91.96 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 12058624 sequences have been processed. [bwa_read_seq] 13.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.70 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 12320768 sequences have been processed. [bwa_read_seq] 14.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 61.46 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 12582912 sequences have been processed. [bwa_read_seq] 3.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 88.56 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 12845056 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 90.52 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 13107200 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.89 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 13369344 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.59 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 13631488 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 91.21 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 13893632 sequences have been processed. [bwa_read_seq] 5.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.65 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 14155776 sequences have been processed. [bwa_read_seq] 5.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.99 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 14417920 sequences have been processed. [bwa_read_seq] 7.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 101.17 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 14680064 sequences have been processed. [bwa_read_seq] 8.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 112.19 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 14942208 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 90.76 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 15204352 sequences have been processed. [bwa_read_seq] 3.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.75 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 15466496 sequences have been processed. [bwa_read_seq] 4.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.84 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 15728640 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.80 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 15990784 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.71 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 16252928 sequences have been processed. [bwa_read_seq] 4.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.64 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 16515072 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.59 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 16777216 sequences have been processed. [bwa_read_seq] 6.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 89.14 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 17039360 sequences have been processed. [bwa_read_seq] 8.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 103.07 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 17301504 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 97.30 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 17563648 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.30 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 17825792 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 89.96 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 18087936 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.61 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 18350080 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 80.29 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 18612224 sequences have been processed. [bwa_read_seq] 2.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.25 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 18874368 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.11 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 19136512 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.35 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 19398656 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 77.69 sec [bwa_aln_core] write to the disk... 0.09 sec [bwa_aln_core] 19660800 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.05 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 19922944 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.49 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 20185088 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.79 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 20447232 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.30 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 20709376 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.89 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 20971520 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.17 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 21233664 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.50 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 21495808 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.37 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 21757952 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.20 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 22020096 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.58 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 22282240 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.80 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 22544384 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 78.42 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 22806528 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.68 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 23068672 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.44 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 23330816 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.64 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 23592960 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.14 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 23855104 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 80.95 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 24117248 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.72 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 24379392 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.18 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 24641536 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.15 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 24903680 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.35 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 25165824 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.23 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 25427968 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.94 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 25690112 sequences have been processed. [bwa_read_seq] 2.2% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.54 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 25952256 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.34 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 26214400 sequences have been processed. [bwa_read_seq] 6.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.44 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 26476544 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 86.11 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 26738688 sequences have been processed. [bwa_read_seq] 5.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.48 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 27000832 sequences have been processed. [bwa_read_seq] 6.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.37 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 27262976 sequences have been processed. [bwa_read_seq] 7.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 94.57 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 27525120 sequences have been processed. [bwa_read_seq] 6.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 108.10 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 27787264 sequences have been processed. [bwa_read_seq] 4.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.85 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 28049408 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.67 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 28311552 sequences have been processed. [bwa_read_seq] 5.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.51 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 28573696 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.31 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 28835840 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.01 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 29097984 sequences have been processed. [bwa_read_seq] 7.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 91.81 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 29360128 sequences have been processed. [bwa_read_seq] 7.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 94.57 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 29622272 sequences have been processed. [bwa_read_seq] 3.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.37 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 29884416 sequences have been processed. [bwa_read_seq] 4.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.92 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 30146560 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 82.22 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 30408704 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.71 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 30670848 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 80.76 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 30932992 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.50 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 31195136 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.34 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 31457280 sequences have been processed. [bwa_read_seq] 7.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 90.87 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 31719424 sequences have been processed. [bwa_read_seq] 10.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 95.20 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 31981568 sequences have been processed. [bwa_read_seq] 4.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.92 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 32243712 sequences have been processed. [bwa_read_seq] 3.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 83.84 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 32505856 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.86 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 32768000 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.56 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 33030144 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.07 sec [bwa_aln_core] write to the disk... 0.08 sec [bwa_aln_core] 33292288 sequences have been processed. [bwa_read_seq] 4.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 84.68 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 33554432 sequences have been processed. [bwa_read_seq] 5.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 81.83 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 33816576 sequences have been processed. [bwa_read_seq] 5.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 85.48 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 34078720 sequences have been processed. [bwa_read_seq] 7.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 96.33 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 34340864 sequences have been processed. [bwa_read_seq] 9.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 60.28 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 34481980 sequences have been processed. [main] Version: 0.7.13-r1126 [main] CMD: bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz [main] Real time: 5866.493 sec; CPU: 11356.524 sec | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 350 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 354 export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1" # SYS command. line 360 if [ -f "${PICARDROOT}/picard.jar" ]; then \ MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \ elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \ MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \ elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \ MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \ fi # SYS command. line 369 if [ -f "${MARKDUP}" ]; then \ java -Xmx4G -jar ${MARKDUP} \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ else \ picard MarkDuplicates \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ fi # SYS command. line 381 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 29015 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1 [Mon Nov 14 17:52:35 PST 2016] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Mon Nov 14 17:52:35 PST 2016] Executing as imk1@nandi on Linux 4.4.0-38-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater INFO 2016-11-14 17:52:35 MarkDuplicates Start of doWork freeMemory: 254879200; totalMemory: 257425408; maxMemory: 11453595648 INFO 2016-11-14 17:52:35 MarkDuplicates Reading input file and constructing read end information. INFO 2016-11-14 17:52:35 MarkDuplicates Will retain up to 44052290 data points before spilling to disk. INFO 2016-11-14 17:52:41 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:05s. Time for last 1,000,000: 5s. Last read position: chr1:22,298,551 INFO 2016-11-14 17:52:41 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:52:45 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:10s. Time for last 1,000,000: 4s. Last read position: chr1:41,895,424 INFO 2016-11-14 17:52:45 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:52:50 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:14s. Time for last 1,000,000: 4s. Last read position: chr1:63,396,527 INFO 2016-11-14 17:52:50 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:52:54 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:19s. Time for last 1,000,000: 4s. Last read position: chr1:89,147,707 INFO 2016-11-14 17:52:54 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:53:00 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:25s. Time for last 1,000,000: 6s. Last read position: chr1:115,615,553 INFO 2016-11-14 17:53:00 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:53:06 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:00:30s. Time for last 1,000,000: 5s. Last read position: chr1:164,468,682 INFO 2016-11-14 17:53:06 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:53:12 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:00:37s. Time for last 1,000,000: 6s. Last read position: chr1:189,102,252 INFO 2016-11-14 17:53:12 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:53:17 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:00:41s. Time for last 1,000,000: 4s. Last read position: chr1:213,979,760 INFO 2016-11-14 17:53:17 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:53:21 MarkDuplicates Read 9,000,000 records. Elapsed time: 00:00:46s. Time for last 1,000,000: 4s. Last read position: chr1:231,814,268 INFO 2016-11-14 17:53:21 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:53:29 MarkDuplicates Read 10,000,000 records. Elapsed time: 00:00:54s. Time for last 1,000,000: 7s. Last read position: chr2:3,652,468 INFO 2016-11-14 17:53:29 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:53:35 MarkDuplicates Read 11,000,000 records. Elapsed time: 00:00:59s. Time for last 1,000,000: 5s. Last read position: chr2:39,505,122 INFO 2016-11-14 17:53:35 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:53:44 MarkDuplicates Read 12,000,000 records. Elapsed time: 00:01:09s. Time for last 1,000,000: 9s. Last read position: chr2:73,561,393 INFO 2016-11-14 17:53:44 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:53:49 MarkDuplicates Read 13,000,000 records. Elapsed time: 00:01:14s. Time for last 1,000,000: 4s. Last read position: chr2:120,526,571 INFO 2016-11-14 17:53:49 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:53:55 MarkDuplicates Read 14,000,000 records. Elapsed time: 00:01:20s. Time for last 1,000,000: 5s. Last read position: chr2:160,316,783 INFO 2016-11-14 17:53:55 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:54:03 MarkDuplicates Read 15,000,000 records. Elapsed time: 00:01:28s. Time for last 1,000,000: 8s. Last read position: chr2:195,549,735 INFO 2016-11-14 17:54:03 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:54:08 MarkDuplicates Read 16,000,000 records. Elapsed time: 00:01:33s. Time for last 1,000,000: 5s. Last read position: chr2:230,358,034 INFO 2016-11-14 17:54:08 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:54:13 MarkDuplicates Read 17,000,000 records. Elapsed time: 00:01:38s. Time for last 1,000,000: 4s. Last read position: chr3:16,305,780 INFO 2016-11-14 17:54:13 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:54:19 MarkDuplicates Read 18,000,000 records. Elapsed time: 00:01:43s. Time for last 1,000,000: 5s. Last read position: chr3:49,251,279 INFO 2016-11-14 17:54:19 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:54:25 MarkDuplicates Read 19,000,000 records. Elapsed time: 00:01:49s. Time for last 1,000,000: 6s. Last read position: chr3:84,621,225 INFO 2016-11-14 17:54:25 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:54:33 MarkDuplicates Read 20,000,000 records. Elapsed time: 00:01:58s. Time for last 1,000,000: 8s. Last read position: chr3:128,121,129 INFO 2016-11-14 17:54:33 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:54:38 MarkDuplicates Read 21,000,000 records. Elapsed time: 00:02:02s. Time for last 1,000,000: 4s. Last read position: chr3:165,373,238 INFO 2016-11-14 17:54:38 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:54:43 MarkDuplicates Read 22,000,000 records. Elapsed time: 00:02:07s. Time for last 1,000,000: 4s. Last read position: chr3:195,333,247 INFO 2016-11-14 17:54:43 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:54:48 MarkDuplicates Read 23,000,000 records. Elapsed time: 00:02:13s. Time for last 1,000,000: 5s. Last read position: chr4:37,752,582 INFO 2016-11-14 17:54:48 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:54:53 MarkDuplicates Read 24,000,000 records. Elapsed time: 00:02:18s. Time for last 1,000,000: 5s. Last read position: chr4:81,286,306 INFO 2016-11-14 17:54:53 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:54:58 MarkDuplicates Read 25,000,000 records. Elapsed time: 00:02:23s. Time for last 1,000,000: 5s. Last read position: chr4:119,339,736 INFO 2016-11-14 17:54:58 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:55:08 MarkDuplicates Read 26,000,000 records. Elapsed time: 00:02:33s. Time for last 1,000,000: 9s. Last read position: chr4:158,818,783 INFO 2016-11-14 17:55:08 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:55:12 MarkDuplicates Read 27,000,000 records. Elapsed time: 00:02:37s. Time for last 1,000,000: 4s. Last read position: chr5:6,447,551 INFO 2016-11-14 17:55:12 MarkDuplicates Tracking 16 as yet unmatched pairs. 16 records in RAM. INFO 2016-11-14 17:55:17 MarkDuplicates Read 28,000,000 records. Elapsed time: 00:02:42s. Time for last 1,000,000: 5s. Last read position: chr5:33,219,021 INFO 2016-11-14 17:55:17 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:55:23 MarkDuplicates Read 29,000,000 records. Elapsed time: 00:02:47s. Time for last 1,000,000: 5s. Last read position: chr5:60,694,078 INFO 2016-11-14 17:55:23 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:55:28 MarkDuplicates Read 30,000,000 records. Elapsed time: 00:02:53s. Time for last 1,000,000: 5s. Last read position: chr5:86,336,676 INFO 2016-11-14 17:55:28 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:55:33 MarkDuplicates Read 31,000,000 records. Elapsed time: 00:02:58s. Time for last 1,000,000: 5s. Last read position: chr5:112,930,184 INFO 2016-11-14 17:55:33 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:55:37 MarkDuplicates Read 32,000,000 records. Elapsed time: 00:03:01s. Time for last 1,000,000: 3s. Last read position: chr5:136,852,889 INFO 2016-11-14 17:55:37 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:55:48 MarkDuplicates Read 33,000,000 records. Elapsed time: 00:03:13s. Time for last 1,000,000: 11s. Last read position: chr5:160,271,193 INFO 2016-11-14 17:55:48 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:55:53 MarkDuplicates Read 34,000,000 records. Elapsed time: 00:03:17s. Time for last 1,000,000: 4s. Last read position: chr6:4,126,386 INFO 2016-11-14 17:55:53 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:55:58 MarkDuplicates Read 35,000,000 records. Elapsed time: 00:03:23s. Time for last 1,000,000: 5s. Last read position: chr6:28,666,579 INFO 2016-11-14 17:55:58 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:56:04 MarkDuplicates Read 36,000,000 records. Elapsed time: 00:03:28s. Time for last 1,000,000: 5s. Last read position: chr6:49,527,281 INFO 2016-11-14 17:56:04 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:56:07 MarkDuplicates Read 37,000,000 records. Elapsed time: 00:03:32s. Time for last 1,000,000: 3s. Last read position: chr6:81,728,195 INFO 2016-11-14 17:56:07 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:56:13 MarkDuplicates Read 38,000,000 records. Elapsed time: 00:03:37s. Time for last 1,000,000: 5s. Last read position: chr6:107,535,760 INFO 2016-11-14 17:56:13 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:56:25 MarkDuplicates Read 39,000,000 records. Elapsed time: 00:03:49s. Time for last 1,000,000: 11s. Last read position: chr6:132,364,408 INFO 2016-11-14 17:56:25 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:56:29 MarkDuplicates Read 40,000,000 records. Elapsed time: 00:03:54s. Time for last 1,000,000: 4s. Last read position: chr6:156,918,595 INFO 2016-11-14 17:56:29 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:56:33 MarkDuplicates Read 41,000,000 records. Elapsed time: 00:03:57s. Time for last 1,000,000: 3s. Last read position: chr7:18,636,743 INFO 2016-11-14 17:56:33 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:56:38 MarkDuplicates Read 42,000,000 records. Elapsed time: 00:04:02s. Time for last 1,000,000: 4s. Last read position: chr7:53,850,313 INFO 2016-11-14 17:56:38 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:56:43 MarkDuplicates Read 43,000,000 records. Elapsed time: 00:04:08s. Time for last 1,000,000: 5s. Last read position: chr7:96,503,433 INFO 2016-11-14 17:56:43 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:56:48 MarkDuplicates Read 44,000,000 records. Elapsed time: 00:04:13s. Time for last 1,000,000: 5s. Last read position: chr7:120,651,846 INFO 2016-11-14 17:56:48 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:57:29 MarkDuplicates Read 45,000,000 records. Elapsed time: 00:04:53s. Time for last 1,000,000: 40s. Last read position: chr7:146,401,497 INFO 2016-11-14 17:57:29 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:57:33 MarkDuplicates Read 46,000,000 records. Elapsed time: 00:04:58s. Time for last 1,000,000: 4s. Last read position: chr8:17,030,519 INFO 2016-11-14 17:57:33 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:57:38 MarkDuplicates Read 47,000,000 records. Elapsed time: 00:05:03s. Time for last 1,000,000: 4s. Last read position: chr8:42,750,671 INFO 2016-11-14 17:57:38 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:57:48 MarkDuplicates Read 48,000,000 records. Elapsed time: 00:05:12s. Time for last 1,000,000: 9s. Last read position: chr8:85,385,487 INFO 2016-11-14 17:57:48 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:57:52 MarkDuplicates Read 49,000,000 records. Elapsed time: 00:05:16s. Time for last 1,000,000: 3s. Last read position: chr8:124,358,985 INFO 2016-11-14 17:57:52 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:57:56 MarkDuplicates Read 50,000,000 records. Elapsed time: 00:05:21s. Time for last 1,000,000: 4s. Last read position: chr9:18,183,883 INFO 2016-11-14 17:57:56 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:58:01 MarkDuplicates Read 51,000,000 records. Elapsed time: 00:05:25s. Time for last 1,000,000: 4s. Last read position: chr9:84,163,983 INFO 2016-11-14 17:58:01 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:58:05 MarkDuplicates Read 52,000,000 records. Elapsed time: 00:05:30s. Time for last 1,000,000: 4s. Last read position: chr9:119,875,535 INFO 2016-11-14 17:58:05 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:58:09 MarkDuplicates Read 53,000,000 records. Elapsed time: 00:05:34s. Time for last 1,000,000: 4s. Last read position: chr10:5,661,683 INFO 2016-11-14 17:58:09 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:58:14 MarkDuplicates Read 54,000,000 records. Elapsed time: 00:05:38s. Time for last 1,000,000: 4s. Last read position: chr10:29,748,948 INFO 2016-11-14 17:58:14 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:58:18 MarkDuplicates Read 55,000,000 records. Elapsed time: 00:05:43s. Time for last 1,000,000: 4s. Last read position: chr10:61,280,221 INFO 2016-11-14 17:58:18 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:58:23 MarkDuplicates Read 56,000,000 records. Elapsed time: 00:05:47s. Time for last 1,000,000: 4s. Last read position: chr10:85,305,552 INFO 2016-11-14 17:58:23 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:58:26 MarkDuplicates Read 57,000,000 records. Elapsed time: 00:05:51s. Time for last 1,000,000: 3s. Last read position: chr10:108,129,202 INFO 2016-11-14 17:58:26 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:58:36 MarkDuplicates Read 58,000,000 records. Elapsed time: 00:06:00s. Time for last 1,000,000: 9s. Last read position: chr10:132,730,123 INFO 2016-11-14 17:58:36 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:58:42 MarkDuplicates Read 59,000,000 records. Elapsed time: 00:06:06s. Time for last 1,000,000: 5s. Last read position: chr11:35,359,267 INFO 2016-11-14 17:58:42 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:58:47 MarkDuplicates Read 60,000,000 records. Elapsed time: 00:06:12s. Time for last 1,000,000: 5s. Last read position: chr11:75,696,431 INFO 2016-11-14 17:58:47 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:58:51 MarkDuplicates Read 61,000,000 records. Elapsed time: 00:06:15s. Time for last 1,000,000: 3s. Last read position: chr11:114,414,621 INFO 2016-11-14 17:58:51 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:58:56 MarkDuplicates Read 62,000,000 records. Elapsed time: 00:06:21s. Time for last 1,000,000: 5s. Last read position: chr12:8,900,276 INFO 2016-11-14 17:58:56 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:59:03 MarkDuplicates Read 63,000,000 records. Elapsed time: 00:06:27s. Time for last 1,000,000: 6s. Last read position: chr12:38,863,889 INFO 2016-11-14 17:59:03 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:59:08 MarkDuplicates Read 64,000,000 records. Elapsed time: 00:06:32s. Time for last 1,000,000: 5s. Last read position: chr12:60,598,824 INFO 2016-11-14 17:59:08 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:59:19 MarkDuplicates Read 65,000,000 records. Elapsed time: 00:06:44s. Time for last 1,000,000: 11s. Last read position: chr12:88,520,538 INFO 2016-11-14 17:59:19 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 17:59:23 MarkDuplicates Read 66,000,000 records. Elapsed time: 00:06:48s. Time for last 1,000,000: 3s. Last read position: chr12:112,268,437 INFO 2016-11-14 17:59:23 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:59:28 MarkDuplicates Read 67,000,000 records. Elapsed time: 00:06:53s. Time for last 1,000,000: 4s. Last read position: chr13:23,999,632 INFO 2016-11-14 17:59:28 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 17:59:33 MarkDuplicates Read 68,000,000 records. Elapsed time: 00:06:58s. Time for last 1,000,000: 5s. Last read position: chr13:51,165,870 INFO 2016-11-14 17:59:33 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:59:38 MarkDuplicates Read 69,000,000 records. Elapsed time: 00:07:02s. Time for last 1,000,000: 4s. Last read position: chr13:88,986,369 INFO 2016-11-14 17:59:38 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:59:42 MarkDuplicates Read 70,000,000 records. Elapsed time: 00:07:06s. Time for last 1,000,000: 3s. Last read position: chr14:23,149,367 INFO 2016-11-14 17:59:42 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 17:59:46 MarkDuplicates Read 71,000,000 records. Elapsed time: 00:07:11s. Time for last 1,000,000: 4s. Last read position: chr14:58,140,905 INFO 2016-11-14 17:59:46 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 17:59:52 MarkDuplicates Read 72,000,000 records. Elapsed time: 00:07:16s. Time for last 1,000,000: 5s. Last read position: chr14:91,912,553 INFO 2016-11-14 17:59:52 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 17:59:57 MarkDuplicates Read 73,000,000 records. Elapsed time: 00:07:21s. Time for last 1,000,000: 5s. Last read position: chr15:38,895,095 INFO 2016-11-14 17:59:57 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:00:10 MarkDuplicates Read 74,000,000 records. Elapsed time: 00:07:34s. Time for last 1,000,000: 12s. Last read position: chr15:68,188,367 INFO 2016-11-14 18:00:10 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 18:00:14 MarkDuplicates Read 75,000,000 records. Elapsed time: 00:07:38s. Time for last 1,000,000: 3s. Last read position: chr15:87,921,272 INFO 2016-11-14 18:00:14 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:00:18 MarkDuplicates Read 76,000,000 records. Elapsed time: 00:07:43s. Time for last 1,000,000: 4s. Last read position: chr16:11,143,813 INFO 2016-11-14 18:00:18 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:00:23 MarkDuplicates Read 77,000,000 records. Elapsed time: 00:07:48s. Time for last 1,000,000: 5s. Last read position: chr16:51,391,437 INFO 2016-11-14 18:00:23 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 18:00:29 MarkDuplicates Read 78,000,000 records. Elapsed time: 00:07:53s. Time for last 1,000,000: 5s. Last read position: chr16:84,928,458 INFO 2016-11-14 18:00:29 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:00:32 MarkDuplicates Read 79,000,000 records. Elapsed time: 00:07:57s. Time for last 1,000,000: 3s. Last read position: chr17:18,368,935 INFO 2016-11-14 18:00:32 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:00:38 MarkDuplicates Read 80,000,000 records. Elapsed time: 00:08:02s. Time for last 1,000,000: 5s. Last read position: chr17:44,246,522 INFO 2016-11-14 18:00:38 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:00:43 MarkDuplicates Read 81,000,000 records. Elapsed time: 00:08:08s. Time for last 1,000,000: 5s. Last read position: chr17:66,172,158 INFO 2016-11-14 18:00:43 MarkDuplicates Tracking 10 as yet unmatched pairs. 10 records in RAM. INFO 2016-11-14 18:00:48 MarkDuplicates Read 82,000,000 records. Elapsed time: 00:08:13s. Time for last 1,000,000: 5s. Last read position: chr18:5,619,614 INFO 2016-11-14 18:00:48 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:00:52 MarkDuplicates Read 83,000,000 records. Elapsed time: 00:08:16s. Time for last 1,000,000: 3s. Last read position: chr18:46,152,631 INFO 2016-11-14 18:00:52 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:00:57 MarkDuplicates Read 84,000,000 records. Elapsed time: 00:08:22s. Time for last 1,000,000: 5s. Last read position: chr19:1,328,731 INFO 2016-11-14 18:00:57 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:01:12 MarkDuplicates Read 85,000,000 records. Elapsed time: 00:08:37s. Time for last 1,000,000: 14s. Last read position: chr19:28,148,444 INFO 2016-11-14 18:01:12 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:01:17 MarkDuplicates Read 86,000,000 records. Elapsed time: 00:08:42s. Time for last 1,000,000: 5s. Last read position: chr19:40,341,579 INFO 2016-11-14 18:01:17 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 18:01:21 MarkDuplicates Read 87,000,000 records. Elapsed time: 00:08:46s. Time for last 1,000,000: 3s. Last read position: chr20:3,222,035 INFO 2016-11-14 18:01:21 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:01:25 MarkDuplicates Read 88,000,000 records. Elapsed time: 00:08:50s. Time for last 1,000,000: 4s. Last read position: chr20:37,218,903 INFO 2016-11-14 18:01:25 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:02:02 MarkDuplicates Read 89,000,000 records. Elapsed time: 00:09:27s. Time for last 1,000,000: 36s. Last read position: chr20:62,871,367 INFO 2016-11-14 18:02:02 MarkDuplicates Tracking 10 as yet unmatched pairs. 10 records in RAM. INFO 2016-11-14 18:02:07 MarkDuplicates Read 90,000,000 records. Elapsed time: 00:09:31s. Time for last 1,000,000: 4s. Last read position: chr21:39,474,524 INFO 2016-11-14 18:02:07 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:02:11 MarkDuplicates Read 91,000,000 records. Elapsed time: 00:09:35s. Time for last 1,000,000: 3s. Last read position: chr22:31,346,944 INFO 2016-11-14 18:02:11 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-14 18:02:16 MarkDuplicates Read 92,000,000 records. Elapsed time: 00:09:40s. Time for last 1,000,000: 5s. Last read position: chrX:6,235,951 INFO 2016-11-14 18:02:16 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 18:02:21 MarkDuplicates Read 93,000,000 records. Elapsed time: 00:09:46s. Time for last 1,000,000: 5s. Last read position: chrX:38,820,975 INFO 2016-11-14 18:02:21 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-14 18:02:27 MarkDuplicates Read 94,000,000 records. Elapsed time: 00:09:52s. Time for last 1,000,000: 5s. Last read position: chrX:69,473,954 INFO 2016-11-14 18:02:27 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:02:31 MarkDuplicates Read 95,000,000 records. Elapsed time: 00:09:55s. Time for last 1,000,000: 3s. Last read position: chrX:92,683,143 INFO 2016-11-14 18:02:31 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-14 18:02:36 MarkDuplicates Read 96,000,000 records. Elapsed time: 00:10:01s. Time for last 1,000,000: 5s. Last read position: chrX:118,447,518 INFO 2016-11-14 18:02:36 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-14 18:02:44 MarkDuplicates Read 97,000,000 records. Elapsed time: 00:10:09s. Time for last 1,000,000: 8s. Last read position: chrX:142,819,613 INFO 2016-11-14 18:02:44 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 18:02:48 MarkDuplicates Read 98,000,000 records. Elapsed time: 00:10:13s. Time for last 1,000,000: 4s. Last read position: chrM:3,995 INFO 2016-11-14 18:02:48 MarkDuplicates Tracking 7472 as yet unmatched pairs. 7472 records in RAM. INFO 2016-11-14 18:02:52 MarkDuplicates Read 99,000,000 records. Elapsed time: 00:10:16s. Time for last 1,000,000: 3s. Last read position: chrM:11,775 INFO 2016-11-14 18:02:52 MarkDuplicates Tracking 14000 as yet unmatched pairs. 14000 records in RAM. INFO 2016-11-14 18:02:56 MarkDuplicates Read 99989486 records. 0 pairs never matched. INFO 2016-11-14 18:03:02 MarkDuplicates After buildSortedReadEndLists freeMemory: 6202756424; totalMemory: 6277300224; maxMemory: 11453595648 INFO 2016-11-14 18:03:02 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk. INFO 2016-11-14 18:03:03 MarkDuplicates Traversing read pair information and detecting duplicates. INFO 2016-11-14 18:03:20 MarkDuplicates Traversing fragment information and detecting duplicates. INFO 2016-11-14 18:03:43 MarkDuplicates Sorting list of duplicate records. INFO 2016-11-14 18:03:44 MarkDuplicates After generateDuplicateIndexes freeMemory: 6192710896; totalMemory: 9131524096; maxMemory: 11453595648 INFO 2016-11-14 18:03:44 MarkDuplicates Marking 496224 records as duplicates. INFO 2016-11-14 18:03:44 MarkDuplicates Found 42648 optical duplicate clusters. INFO 2016-11-14 18:05:54 MarkDuplicates Written 10,000,000 records. Elapsed time: 00:02:09s. Time for last 10,000,000: 129s. Last read position: chr2:3,652,468 INFO 2016-11-14 18:08:13 MarkDuplicates Written 20,000,000 records. Elapsed time: 00:04:28s. Time for last 10,000,000: 139s. Last read position: chr3:128,121,129 INFO 2016-11-14 18:10:29 MarkDuplicates Written 30,000,000 records. Elapsed time: 00:06:44s. Time for last 10,000,000: 135s. Last read position: chr5:86,336,676 INFO 2016-11-14 18:12:46 MarkDuplicates Written 40,000,000 records. Elapsed time: 00:09:01s. Time for last 10,000,000: 137s. Last read position: chr6:156,918,595 INFO 2016-11-14 18:15:05 MarkDuplicates Written 50,000,000 records. Elapsed time: 00:11:20s. Time for last 10,000,000: 139s. Last read position: chr9:18,183,883 INFO 2016-11-14 18:17:29 MarkDuplicates Written 60,000,000 records. Elapsed time: 00:13:44s. Time for last 10,000,000: 143s. Last read position: chr11:75,696,431 INFO 2016-11-14 18:19:53 MarkDuplicates Written 70,000,000 records. Elapsed time: 00:16:08s. Time for last 10,000,000: 143s. Last read position: chr14:23,149,367 INFO 2016-11-14 18:22:27 MarkDuplicates Written 80,000,000 records. Elapsed time: 00:18:43s. Time for last 10,000,000: 154s. Last read position: chr17:44,246,522 INFO 2016-11-14 18:24:44 MarkDuplicates Written 90,000,000 records. Elapsed time: 00:20:59s. Time for last 10,000,000: 136s. Last read position: chr21:39,474,524 INFO 2016-11-14 18:26:55 MarkDuplicates Before output close freeMemory: 9212729248; totalMemory: 9297199104; maxMemory: 11453595648 INFO 2016-11-14 18:26:57 MarkDuplicates After output close freeMemory: 9212707392; totalMemory: 9297199104; maxMemory: 11453595648 [Mon Nov 14 18:26:59 PST 2016] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 34.40 minutes. Runtime.totalMemory()=9297199104 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 146 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 150 samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam # SYS command. line 153 sambamba index -t 3 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam # SYS command. line 155 sambamba flagstat -t 3 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/ctl1/ss_50M_2000_GRCh38.nodup.flagstat.qc # SYS command. line 165 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam | \ awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \ grep -v 'chrM' | sort | uniq -c | \ awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/ctl1/ss_50M_2000_GRCh38.nodup.pbc.qc # SYS command. line 169 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 53475 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 142 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 147 bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz | samtools view -Su /dev/stdin \ | sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.bam # SYS command. line 149 sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.flagstat.qc # SYS command. line 151 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 26486 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 45.91 sec [bwa_aln_core] refine gapped alignments... 6.37 sec [bwa_aln_core] print alignments... [samopen] SAM header is present: 493 sequences. 0.44 sec [bwa_aln_core] 262144 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 36.69 sec [bwa_aln_core] refine gapped alignments... 4.63 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 524288 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 31.66 sec [bwa_aln_core] refine gapped alignments... 5.00 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 786432 sequences have been processed. [bwa_read_seq] 2.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 32.19 sec [bwa_aln_core] refine gapped alignments... 5.32 sec [bwa_aln_core] print alignments... 0.61 sec [bwa_aln_core] 1048576 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 40.06 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 1310720 sequences have been processed. [bwa_read_seq] 3.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.69 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.72 sec [bwa_aln_core] 1572864 sequences have been processed. [bwa_read_seq] 2.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 37.02 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.88 sec [bwa_aln_core] 1835008 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 31.36 sec [bwa_aln_core] refine gapped alignments... 0.53 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 2097152 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 34.84 sec [bwa_aln_core] refine gapped alignments... 0.66 sec [bwa_aln_core] print alignments... 0.69 sec [bwa_aln_core] 2359296 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.02 sec [bwa_aln_core] refine gapped alignments... 0.68 sec [bwa_aln_core] print alignments... 0.95 sec [bwa_aln_core] 2621440 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.37 sec [bwa_aln_core] refine gapped alignments... 0.75 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 2883584 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.94 sec [bwa_aln_core] refine gapped alignments... 7.36 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 3145728 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 31.88 sec [bwa_aln_core] refine gapped alignments... 1.40 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 3407872 sequences have been processed. [bwa_read_seq] 2.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 34.32 sec [bwa_aln_core] refine gapped alignments... 2.55 sec [bwa_aln_core] print alignments... 0.64 sec [bwa_aln_core] 3670016 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 36.68 sec [bwa_aln_core] refine gapped alignments... 1.95 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 3932160 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 37.56 sec [bwa_aln_core] refine gapped alignments... 2.37 sec [bwa_aln_core] print alignments... 0.74 sec [bwa_aln_core] 4194304 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 38.95 sec [bwa_aln_core] refine gapped alignments... 5.85 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 4456448 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 37.77 sec [bwa_aln_core] refine gapped alignments... 3.77 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 4718592 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 48.35 sec [bwa_aln_core] refine gapped alignments... 4.40 sec [bwa_aln_core] print alignments... 0.42 sec [bwa_aln_core] 4980736 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 53.09 sec [bwa_aln_core] refine gapped alignments... 6.66 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 5242880 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 52.24 sec [bwa_aln_core] refine gapped alignments... 5.20 sec [bwa_aln_core] print alignments... 0.93 sec [bwa_aln_core] 5505024 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 49.20 sec [bwa_aln_core] refine gapped alignments... 3.07 sec [bwa_aln_core] print alignments... 0.95 sec [bwa_aln_core] 5767168 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 39.34 sec [bwa_aln_core] refine gapped alignments... 2.39 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 6029312 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 31.67 sec [bwa_aln_core] refine gapped alignments... 2.61 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 6291456 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.13 sec [bwa_aln_core] refine gapped alignments... 2.83 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 6553600 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 37.16 sec [bwa_aln_core] refine gapped alignments... 3.04 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 6815744 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 30.22 sec [bwa_aln_core] refine gapped alignments... 2.99 sec [bwa_aln_core] print alignments... 0.42 sec [bwa_aln_core] 7077888 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 22.05 sec [bwa_aln_core] refine gapped alignments... 7.12 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 7340032 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 40.45 sec [bwa_aln_core] refine gapped alignments... 0.69 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 7602176 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 31.38 sec [bwa_aln_core] refine gapped alignments... 0.65 sec [bwa_aln_core] print alignments... 0.61 sec [bwa_aln_core] 7864320 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.06 sec [bwa_aln_core] refine gapped alignments... 0.64 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 8126464 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.37 sec [bwa_aln_core] refine gapped alignments... 1.03 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 8388608 sequences have been processed. [bwa_read_seq] 2.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.15 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 8650752 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 21.22 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 8912896 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.68 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 9175040 sequences have been processed. [bwa_read_seq] 5.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.75 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.65 sec [bwa_aln_core] 9437184 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.73 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 9699328 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.78 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 9961472 sequences have been processed. [bwa_read_seq] 7.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.71 sec [bwa_aln_core] refine gapped alignments... 0.53 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 10223616 sequences have been processed. [bwa_read_seq] 8.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.56 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 10485760 sequences have been processed. [bwa_read_seq] 13.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.16 sec [bwa_aln_core] refine gapped alignments... 1.20 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 10747904 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.52 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 11010048 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.24 sec [bwa_aln_core] refine gapped alignments... 0.68 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 11272192 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.61 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 11534336 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.57 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 11796480 sequences have been processed. [bwa_read_seq] 6.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.49 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 12058624 sequences have been processed. [bwa_read_seq] 13.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.45 sec [bwa_aln_core] refine gapped alignments... 0.93 sec [bwa_aln_core] print alignments... 0.46 sec [bwa_aln_core] 12320768 sequences have been processed. [bwa_read_seq] 14.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.61 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 12582912 sequences have been processed. [bwa_read_seq] 3.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.55 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 12845056 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.19 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 13107200 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.58 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 13369344 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.69 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 13631488 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.63 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 13893632 sequences have been processed. [bwa_read_seq] 5.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.07 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 14155776 sequences have been processed. [bwa_read_seq] 5.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.11 sec [bwa_aln_core] refine gapped alignments... 0.63 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 14417920 sequences have been processed. [bwa_read_seq] 7.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.76 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 14680064 sequences have been processed. [bwa_read_seq] 8.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.21 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 14942208 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.27 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 15204352 sequences have been processed. [bwa_read_seq] 3.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.33 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 15466496 sequences have been processed. [bwa_read_seq] 4.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.20 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 15728640 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.97 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.69 sec [bwa_aln_core] 15990784 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.79 sec [bwa_aln_core] refine gapped alignments... 1.88 sec [bwa_aln_core] print alignments... 0.63 sec [bwa_aln_core] 16252928 sequences have been processed. [bwa_read_seq] 4.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.55 sec [bwa_aln_core] refine gapped alignments... 1.02 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 16515072 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.14 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 16777216 sequences have been processed. [bwa_read_seq] 6.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.69 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 17039360 sequences have been processed. [bwa_read_seq] 8.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.63 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 17301504 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.82 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 17563648 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.19 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 17825792 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 9.86 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 18087936 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.88 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 18350080 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.78 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 18612224 sequences have been processed. [bwa_read_seq] 2.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.79 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 18874368 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.12 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 19136512 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.45 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 19398656 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.08 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 19660800 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.44 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 19922944 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.22 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 20185088 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.61 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 20447232 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.38 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 20709376 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.21 sec [bwa_aln_core] refine gapped alignments... 0.66 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 20971520 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.57 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 21233664 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.75 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 21495808 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.65 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 21757952 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.59 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 22020096 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.97 sec [bwa_aln_core] refine gapped alignments... 0.67 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 22282240 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.96 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 22544384 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.70 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 22806528 sequences have been processed. [bwa_read_seq] 1.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.81 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 23068672 sequences have been processed. [bwa_read_seq] 2.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 12.20 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 23330816 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.71 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 23592960 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.80 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 23855104 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.77 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 24117248 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.59 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 24379392 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.21 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 24641536 sequences have been processed. [bwa_read_seq] 1.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.06 sec [bwa_aln_core] refine gapped alignments... 0.65 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 24903680 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.22 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 25165824 sequences have been processed. [bwa_read_seq] 1.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.35 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 25427968 sequences have been processed. [bwa_read_seq] 2.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.91 sec [bwa_aln_core] refine gapped alignments... 1.95 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 25690112 sequences have been processed. [bwa_read_seq] 2.2% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 10.92 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 25952256 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.24 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 26214400 sequences have been processed. [bwa_read_seq] 6.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.22 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 26476544 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.74 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 26738688 sequences have been processed. [bwa_read_seq] 5.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 11.91 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 27000832 sequences have been processed. [bwa_read_seq] 6.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 12.78 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.61 sec [bwa_aln_core] 27262976 sequences have been processed. [bwa_read_seq] 7.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.59 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 27525120 sequences have been processed. [bwa_read_seq] 6.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.46 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 27787264 sequences have been processed. [bwa_read_seq] 4.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.64 sec [bwa_aln_core] refine gapped alignments... 0.69 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 28049408 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 18.71 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 28311552 sequences have been processed. [bwa_read_seq] 5.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 18.11 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 28573696 sequences have been processed. [bwa_read_seq] 5.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 13.85 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 28835840 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.14 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 29097984 sequences have been processed. [bwa_read_seq] 7.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.19 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 29360128 sequences have been processed. [bwa_read_seq] 7.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.00 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 29622272 sequences have been processed. [bwa_read_seq] 3.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 19.58 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 29884416 sequences have been processed. [bwa_read_seq] 4.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.43 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 30146560 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 17.44 sec [bwa_aln_core] refine gapped alignments... 0.63 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 30408704 sequences have been processed. [bwa_read_seq] 4.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 19.10 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 30670848 sequences have been processed. [bwa_read_seq] 4.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.55 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 30932992 sequences have been processed. [bwa_read_seq] 5.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.78 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 31195136 sequences have been processed. [bwa_read_seq] 5.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 20.55 sec [bwa_aln_core] refine gapped alignments... 1.77 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 31457280 sequences have been processed. [bwa_read_seq] 7.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.83 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.61 sec [bwa_aln_core] 31719424 sequences have been processed. [bwa_read_seq] 10.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.76 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 31981568 sequences have been processed. [bwa_read_seq] 4.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.80 sec [bwa_aln_core] refine gapped alignments... 1.31 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 32243712 sequences have been processed. [bwa_read_seq] 3.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 22.81 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.46 sec [bwa_aln_core] 32505856 sequences have been processed. [bwa_read_seq] 4.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.87 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 32768000 sequences have been processed. [bwa_read_seq] 4.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 16.42 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.62 sec [bwa_aln_core] 33030144 sequences have been processed. [bwa_read_seq] 4.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 17.71 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.64 sec [bwa_aln_core] 33292288 sequences have been processed. [bwa_read_seq] 4.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 21.19 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 33554432 sequences have been processed. [bwa_read_seq] 5.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 15.81 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 33816576 sequences have been processed. [bwa_read_seq] 5.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 16.38 sec [bwa_aln_core] refine gapped alignments... 0.72 sec [bwa_aln_core] print alignments... 0.63 sec [bwa_aln_core] 34078720 sequences have been processed. [bwa_read_seq] 7.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 16.43 sec [bwa_aln_core] refine gapped alignments... 0.68 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 34340864 sequences have been processed. [bwa_read_seq] 9.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 17.02 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.27 sec [bwa_aln_core] 34481980 sequences have been processed. [main] Version: 0.7.13-r1126 [main] CMD: bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz [main] Real time: 2937.885 sec; CPU: 2735.720 sec | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 507 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 510 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.tagAlign.gz # SYS command. line 512 echo # SYS command. line 514 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 55366 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 97 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 105 if [[ 0 > 0 ]]; then \ sambamba sort -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.qnmsrt.bam; \ samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \ samtools view -F 1804 -Su /dev/stdin | \ sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.filt.bam; \ rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.qnmsrt.bam; \ else \ samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.bam | \ sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.filt.bam; \ fi # SYS command. line 116 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 27085 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 350 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 354 export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1" # SYS command. line 360 if [ -f "${PICARDROOT}/picard.jar" ]; then \ MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \ elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \ MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \ elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \ MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \ fi # SYS command. line 369 if [ -f "${MARKDUP}" ]; then \ java -Xmx4G -jar ${MARKDUP} \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ else \ picard MarkDuplicates \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ fi # SYS command. line 381 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 7712 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1 [Mon Nov 14 20:07:41 PST 2016] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Mon Nov 14 20:07:41 PST 2016] Executing as imk1@kali on Linux 3.19.0-64-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater INFO 2016-11-14 20:07:41 MarkDuplicates Start of doWork freeMemory: 254879344; totalMemory: 257425408; maxMemory: 11453595648 INFO 2016-11-14 20:07:41 MarkDuplicates Reading input file and constructing read end information. INFO 2016-11-14 20:07:41 MarkDuplicates Will retain up to 44052290 data points before spilling to disk. INFO 2016-11-14 20:08:04 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:17s. Time for last 1,000,000: 17s. Last read position: chr1:78,741,356 INFO 2016-11-14 20:08:04 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:08:17 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:30s. Time for last 1,000,000: 12s. Last read position: chr1:185,588,179 INFO 2016-11-14 20:08:17 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:08:31 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:44s. Time for last 1,000,000: 14s. Last read position: chr2:15,959,887 INFO 2016-11-14 20:08:31 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:08:48 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:01:01s. Time for last 1,000,000: 17s. Last read position: chr2:149,200,254 INFO 2016-11-14 20:08:48 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:09:00 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:01:13s. Time for last 1,000,000: 12s. Last read position: chr3:18,700,315 INFO 2016-11-14 20:09:00 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:09:14 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:01:27s. Time for last 1,000,000: 14s. Last read position: chr3:141,964,409 INFO 2016-11-14 20:09:14 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:09:27 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:01:40s. Time for last 1,000,000: 12s. Last read position: chr4:75,456,986 INFO 2016-11-14 20:09:27 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:09:43 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:01:56s. Time for last 1,000,000: 16s. Last read position: chr5:6,751,231 INFO 2016-11-14 20:09:43 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:09:55 MarkDuplicates Read 9,000,000 records. Elapsed time: 00:02:08s. Time for last 1,000,000: 11s. Last read position: chr5:89,274,961 INFO 2016-11-14 20:09:55 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:10:00 MarkDuplicates Read 10,000,000 records. Elapsed time: 00:02:13s. Time for last 1,000,000: 4s. Last read position: chr5:170,726,017 INFO 2016-11-14 20:10:00 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:10:26 MarkDuplicates Read 11,000,000 records. Elapsed time: 00:02:39s. Time for last 1,000,000: 26s. Last read position: chr6:78,094,157 INFO 2016-11-14 20:10:26 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:10:31 MarkDuplicates Read 12,000,000 records. Elapsed time: 00:02:44s. Time for last 1,000,000: 4s. Last read position: chr6:158,203,945 INFO 2016-11-14 20:10:31 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:10:46 MarkDuplicates Read 13,000,000 records. Elapsed time: 00:02:59s. Time for last 1,000,000: 15s. Last read position: chr7:105,680,332 INFO 2016-11-14 20:10:46 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:10:59 MarkDuplicates Read 14,000,000 records. Elapsed time: 00:03:12s. Time for last 1,000,000: 12s. Last read position: chr8:38,385,170 INFO 2016-11-14 20:10:59 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:04 MarkDuplicates Read 15,000,000 records. Elapsed time: 00:03:17s. Time for last 1,000,000: 4s. Last read position: chr9:19,294,109 INFO 2016-11-14 20:11:04 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:13 MarkDuplicates Read 16,000,000 records. Elapsed time: 00:03:26s. Time for last 1,000,000: 9s. Last read position: chr10:17,001,249 INFO 2016-11-14 20:11:13 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:17 MarkDuplicates Read 17,000,000 records. Elapsed time: 00:03:30s. Time for last 1,000,000: 4s. Last read position: chr10:105,842,191 INFO 2016-11-14 20:11:17 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:30 MarkDuplicates Read 18,000,000 records. Elapsed time: 00:03:43s. Time for last 1,000,000: 12s. Last read position: chr11:86,579,224 INFO 2016-11-14 20:11:30 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:36 MarkDuplicates Read 19,000,000 records. Elapsed time: 00:03:49s. Time for last 1,000,000: 5s. Last read position: chr12:53,758,155 INFO 2016-11-14 20:11:36 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:43 MarkDuplicates Read 20,000,000 records. Elapsed time: 00:03:56s. Time for last 1,000,000: 7s. Last read position: chr13:23,633,964 INFO 2016-11-14 20:11:43 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:48 MarkDuplicates Read 21,000,000 records. Elapsed time: 00:04:01s. Time for last 1,000,000: 5s. Last read position: chr14:50,460,993 INFO 2016-11-14 20:11:48 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:11:56 MarkDuplicates Read 22,000,000 records. Elapsed time: 00:04:09s. Time for last 1,000,000: 7s. Last read position: chr15:75,367,961 INFO 2016-11-14 20:11:56 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:00 MarkDuplicates Read 23,000,000 records. Elapsed time: 00:04:13s. Time for last 1,000,000: 4s. Last read position: chr16:74,774,532 INFO 2016-11-14 20:12:00 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:15 MarkDuplicates Read 24,000,000 records. Elapsed time: 00:04:28s. Time for last 1,000,000: 15s. Last read position: chr17:74,776,409 INFO 2016-11-14 20:12:15 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:20 MarkDuplicates Read 25,000,000 records. Elapsed time: 00:04:33s. Time for last 1,000,000: 4s. Last read position: chr19:19,270,904 INFO 2016-11-14 20:12:20 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:27 MarkDuplicates Read 26,000,000 records. Elapsed time: 00:04:40s. Time for last 1,000,000: 7s. Last read position: chr20:44,059,213 INFO 2016-11-14 20:12:27 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:36 MarkDuplicates Read 27,000,000 records. Elapsed time: 00:04:49s. Time for last 1,000,000: 9s. Last read position: chrX:20,247,860 INFO 2016-11-14 20:12:36 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:41 MarkDuplicates Read 28,000,000 records. Elapsed time: 00:04:54s. Time for last 1,000,000: 4s. Last read position: chrX:114,957,430 INFO 2016-11-14 20:12:41 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-14 20:12:44 MarkDuplicates Read 28524782 records. 0 pairs never matched. INFO 2016-11-14 20:12:55 MarkDuplicates After buildSortedReadEndLists freeMemory: 4027538792; totalMemory: 6284640256; maxMemory: 11453595648 INFO 2016-11-14 20:12:55 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk. INFO 2016-11-14 20:12:55 MarkDuplicates Traversing read pair information and detecting duplicates. INFO 2016-11-14 20:12:55 MarkDuplicates Traversing fragment information and detecting duplicates. INFO 2016-11-14 20:12:57 MarkDuplicates Sorting list of duplicate records. INFO 2016-11-14 20:12:59 MarkDuplicates After generateDuplicateIndexes freeMemory: 3343023552; totalMemory: 6284640256; maxMemory: 11453595648 INFO 2016-11-14 20:12:59 MarkDuplicates Marking 1663351 records as duplicates. INFO 2016-11-14 20:12:59 MarkDuplicates Found 0 optical duplicate clusters. INFO 2016-11-14 20:15:59 MarkDuplicates Written 10,000,000 records. Elapsed time: 00:03:00s. Time for last 10,000,000: 180s. Last read position: chr5:170,726,017 INFO 2016-11-14 20:18:49 MarkDuplicates Written 20,000,000 records. Elapsed time: 00:05:49s. Time for last 10,000,000: 169s. Last read position: chr13:23,633,964 INFO 2016-11-14 20:21:15 MarkDuplicates Before output close freeMemory: 9107335824; totalMemory: 9190244352; maxMemory: 11453595648 INFO 2016-11-14 20:21:16 MarkDuplicates After output close freeMemory: 9004049928; totalMemory: 9086959616; maxMemory: 11453595648 [Mon Nov 14 20:21:16 PST 2016] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 13.59 minutes. Runtime.totalMemory()=9086959616 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 146 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 150 samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.bam # SYS command. line 153 sambamba index -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.bam # SYS command. line 155 sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.flagstat.qc # SYS command. line 165 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.dupmark.bam | \ awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \ grep -v 'chrM' | sort | uniq -c | \ awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.pbc.qc # SYS command. line 169 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 45047 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 507 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 510 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz # SYS command. line 512 echo # SYS command. line 514 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 42366 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 47 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 50 zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz | \ grep -v "chrM" | shuf -n 15000000 --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.15M.tagAlign.gz # SYS command. line 53 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 42570 (process ID) old priority 0, new priority 10 Waiting for 30 seconds. | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 257 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 260 nlines=$( zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz | wc -l ) # SYS command. line 261 nlines=$(( (nlines + 1) / 2 )) # SYS command. line 265 zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz | shuf --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.tagAlign.gz | split -d -l $((nlines)) - /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup. # SYS command. line 268 gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup.00 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup.pr1.tagAlign.gz # SYS command. line 269 rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup.00 # SYS command. line 270 gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup.01 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr2/SRR1370878_1.nodup.pr2.tagAlign.gz # SYS command. line 271 rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/pseudo_reps/rep1/pr1/SRR1370878_1.nodup.01 # SYS command. line 273 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 42742 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 214 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 217 if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R); \ else RUN_SPP=$(which run_spp.R); \ fi # SYS command. line 223 Rscript ${RUN_SPP} -rf \ -c=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.15M.tagAlign.gz -p=2 \ -filtchr=chrM -savp=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.plot.pdf -out=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc # SYS command. line 226 sed -r 's/,[^\t]+//g' /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc.tmp # SYS command. line 227 mv /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc.tmp /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc # SYS command. line 229 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 24291 (process ID) old priority 0, new priority 10 ################ ChIP data: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.15M.tagAlign.gz Control data: NA strandshift(min): -500 strandshift(step): 5 strandshift(max) 1500 user-defined peak shift NA exclusion(min): 10 exclusion(max): NaN num parallel nodes: 2 FDR threshold: 0.01 NumPeaks Threshold: NA Output Directory: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1 narrowPeak output file name: NA regionPeak output file name: NA Rdata filename: NA plot pdf filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.plot.pdf result filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.qc Overwrite files?: TRUE Decompressing ChIP file Reading ChIP tagAlign/BAM file /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/align/rep1/SRR1370878_1.nodup.15M.tagAlign.gz opened /tmp/12097.1.q/RtmpK00lr1/SRR1370878_1.nodup.15M.tagAlign5f77329002f done. read 15000000 fragments ChIP data read length 109 [1] TRUE Calculating peak characteristics Minimum cross-correlation value 0.1731978 Minimum cross-correlation shift 1500 Top 3 cross-correlation values 0.203041116457123 Top 3 estimates for fragment length 160 Window half size 385 Phantom peak location 115 Phantom peak Correlation 0.1952309 Normalized Strand cross-correlation coefficient (NSC) 1.172308 Relative Strand cross-correlation Coefficient (RSC) 1.354474 Phantom Peak Quality Tag 1 null device 1 --------------------Stderr-------------------- Loading required package: caTools Warning message: package ‘caTools’ was built under R version 3.2.3 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 98 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 99 dot -Tsvg /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/report/SRR1370878_1_workflow.dot > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/report/SRR1370878_1_workflow.svg 2> /dev/null || echo "svg: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/report/SRR1370878_1_workflow.svg" # to suppress dot font error (exit code=1) --------------------Stdout-------------------- 44941 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 325 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 326 gs -dFirstPage=1 -dLastPage=1 -dTextAlphaBits=4 -dGraphicsAlphaBits=4 -r110x110 \ -dUseCropBox -dQUIET -dSAFER -dBATCH -dNOPAUSE -dNOPROMPT -sDEVICE=png16m \ -sOutputFile=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.plot.png \ -r144 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out/qc/rep1/SRR1370878_1.nodup.15M.cc.plot.pdf --------------------Stdout-------------------- 44977 (process ID) old priority 0, new priority 10 |
Type | Name | Value |
---|---|---|
string | _ | /usr/bin/bds |
string | _LMFILES_ | /software/modulefiles/modsappsdir/modsappsdir:/software/modulefiles/sratoolkit/2.5.2:/software/modulefiles/ascp/3.5.6 |
bool | allowEmpty | false |
string[] | args | [-title, SRR1370878_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370878_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
string | ASCPKEY | /software/ascp/etc/asperaweb_id_dsa.openssh |
bool | canFail | false |
int | cpus | -1 |
int | cpusLocal | 56 |
int | day | 86400 |
string | DEEPLIFT_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/deeplift_private/deeplift |
string | DERBY_HOME | /usr/lib/jvm/java-8-oracle/db |
string | DISPLAY | localhost:12.0 |
real | E | 2.718281828459045 |
string | ENHANCER_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/enhancer_prediction_code/ |
int | G | 1073741824 |
string | HOME | /users/imk1/ |
int | hour | 3600 |
string | J2REDIR | /usr/lib/jvm/java-8-oracle/jre |
string | J2SDKDIR | /usr/lib/jvm/java-8-oracle |
string | JAVA_HOME | /usr/lib/jvm/java-8-oracle |
int | K | 1024 |
string | KERAS_DIR | /users/imk1/.local/lib/python2.7/site-packages/keras/ |
string | KRB5CCNAME | FILE:/tmp/krb5cc_1048_N5SeQf |
string | LANG | en_US.UTF-8 |
string | LOADEDMODULES | modsappsdir/modsappsdir:sratoolkit/2.5.2:ascp/3.5.6 |
string | LOGNAME | imk1 |
int | M | 1048576 |
string | /var/mail/imk1 | |
int | mem | -1 |
int | minute | 60 |
string | MODULE_VERSION | 3.2.10 |
string | MODULE_VERSION_STACK | 3.2.10 |
string | MODULEPATH | /usr/local/Modules/versions\t\t\t\t:/usr/local/Modules/$MODULE_VERSION/modulefiles\t:/modules/\t\t\t\t:/software/modulefiles |
string | MODULESAPPSDIR | /software |
string | MODULESHOME | /software/env_module/3.2.10 |
string | NLSPATH | /usr/dt/lib/nls/msg/%L/%N.cat |
string | node | |
int | P | 1125899906842624 |
string | PATH | /users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/software/ascp/:/software/sratoolkit/2.5.2/bin/:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/software/miniconda3/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect |
string | PERL5LIB | /users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5 |
string | PERL_LOCAL_LIB_ROOT | /users/imk1/perl5:/users/imk1/perl5:/users/imk1/perl5:/users/imk1/perl5 |
string | PERL_MB_OPT | --install_base \"/users/imk1/perl5\" |
string | PERL_MM_OPT | INSTALL_BASE=/users/imk1/perl5 |
real | PI | 3.141592653589793 |
string | ppwd | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1 |
string | programName | chipseq.bds |
string | programPath | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/chipseq.bds |
string | PWD | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370878_1 |
string | PYTHONPATH | /users/imk1/.local/lib/python2.7/site-packages/keras/:/srv/scratch/imk1/TFBindingPredictionProject/src/pybedtools/ |
string | queue | |
int | retry | 0 |
string | RULEFITBASE | /srv/scratch/imk1/TFBindingPredictionProject/src/RuleFit/ |
string | SHELL | /bin/bash |
string | SHLVL | 3 |
string | SSH_CLIENT | 171.65.77.8 55144 22 |
string | SSH_CONNECTION | 171.65.77.8 55144 171.65.76.63 22 |
string | SSH_TTY | /dev/pts/4 |
string | STY | 22993.SRR1370878_1.BDS |
string | system | sge |
int | T | 1099511627776 |
string | TERM | screen |
string | TERMCAP | SC|screen|VT 100/ANSI X3.64 virtual terminal:\\\n\t:DO=\\E[%dB:LE=\\E[%dD:RI=\\E[%dC:UP=\\E[%dA:bs:bt=\\E[Z:\\\n\t:cd=\\E[J:ce=\\E[K:cl=\\E[H\\E[J:cm=\\E[%i%d;%dH:ct=\\E[3g:\\\n\t:do=^J:nd=\\E[C:pt:rc=\\E8:rs=\\Ec:sc=\\E7:st=\\EH:up=\\EM:\\\n\t:le=^H:bl=^G:cr=^M:it#8:ho=\\E[H:nw=\\EE:ta=^I:is=\\E)0:\\\n\t:li#24:co#80:am:xn:xv:LP:sr=\\EM:al=\\E[L:AL=\\E[%dL:\\\n\t:cs=\\E[%i%d;%dr:dl=\\E[M:DL=\\E[%dM:dc=\\E[P:DC=\\E[%dP:\\\n\t:im=\\E[4h:ei=\\E[4l:mi:IC=\\E[%d@:ks=\\E[?1h\\E=:\\\n\t:ke=\\E[?1l\\E>:vi=\\E[?25l:ve=\\E[34h\\E[?25h:vs=\\E[34l:\\\n\t:ti=\\E[?1049h:te=\\E[?1049l:Km=\\E[M:k0=\\E[10~:k1=\\EOP:\\\n\t:k2=\\EOQ:k3=\\EOR:k4=\\EOS:k5=\\E[15~:k6=\\E[17~:k7=\\E[18~:\\\n\t:k8=\\E[19~:k9=\\E[20~:k;=\\E[21~:F1=\\E[23~:F2=\\E[24~:\\\n\t:kh=\\E[1~:@1=\\E[1~:kH=\\E[4~:@7=\\E[4~:kN=\\E[6~:kP=\\E[5~:\\\n\t:kI=\\E[2~:kD=\\E[3~:ku=\\EOA:kd=\\EOB:kr=\\EOC:kl=\\EOD: |
int | timeout | -1 |
string | USER | imk1 |
string | UTIL_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/av_scripts/ |
int | walltimeout | 8640000 |
int | week | 604800 |
string | WINDOW | 0 |
string | XDG_RUNTIME_DIR | /run/user/1048 |
string | XDG_SESSION_ID | 20 |
string | XFILESEARCHPATH | /usr/dt/app-defaults/%L/Dt |