Script file | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/chipseq.bds |
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Program ID | chipseq.bds.20161115_124820_125 |
Start time | 2016-11-15 12:48:20 |
Run time | 02:26:07.699 |
Tasks executed | 15 |
Tasks failed | 0 |
Tasks failed names | |
Arguments* | [-title, SRR1370903_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
System* | sge |
Cpus* | -1 |
Exit value | 0 |
Thread ID | Parent | Tasks |
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thread_Root | None | chipseq.bds.20161115_124820_125/task.graphviz.report.line_97.id_23 chipseq.bds.20161115_124820_125/task.report.pdf2png.line_324.id_28 |
thread_41 | thread_Root | chipseq.bds.20161115_124820_125_parallel_41/task.align_bwa.bwa_aln_rep1.line_108.id_11 chipseq.bds.20161115_124820_125_parallel_41/task.align_bwa.bwa_sam_rep1.line_140.id_14 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bam.dedup_bam_1_rep1.line_95.id_16 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bam.markdup_bam_picard_rep1.line_348.id_17 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bam.dedup_bam_2_rep1.line_144.id_18 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bam.bam_to_tag_rep1.line_505.id_19 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bed.subsample_tag_rep1.line_45.id_20 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bed.spr_rep1.line_255.id_21 chipseq.bds.20161115_124820_125_parallel_41/task.postalign_bed.xcor_rep1.line_212.id_22 |
thread_42 | thread_Root | chipseq.bds.20161115_124820_125_parallel_42/task.postalign_bam.dedup_bam_1_ctl1.line_95.id_10 chipseq.bds.20161115_124820_125_parallel_42/task.postalign_bam.markdup_bam_picard_ctl1.line_348.id_12 chipseq.bds.20161115_124820_125_parallel_42/task.postalign_bam.dedup_bam_2_ctl1.line_144.id_13 chipseq.bds.20161115_124820_125_parallel_42/task.postalign_bam.bam_to_tag_ctl1.line_505.id_15 |
Task | Execution | Time | Dependencies | Task program, Errors, StdOut / StdErr | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 97 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 105 if [[ 0 > 0 ]]; then \ sambamba sort -t 4 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \ samtools view -F 1804 -Su /dev/stdin | \ sambamba sort -t 4 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ else \ samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam | \ sambamba sort -t 4 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ fi # SYS command. line 116 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 7037 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 110 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 113 bwa aln -q 5 -l 32 -k 2 -t 1 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.sai # SYS command. line 115 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 16710 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- [bwa_aln] 17bp reads: max_diff = 2 [bwa_aln] 38bp reads: max_diff = 3 [bwa_aln] 64bp reads: max_diff = 4 [bwa_aln] 93bp reads: max_diff = 5 [bwa_aln] 124bp reads: max_diff = 6 [bwa_aln] 157bp reads: max_diff = 7 [bwa_aln] 190bp reads: max_diff = 8 [bwa_aln] 225bp reads: max_diff = 9 [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 51.23 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 262144 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 51.10 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 524288 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.22 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 786432 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.79 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1048576 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.97 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1310720 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.92 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1572864 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.85 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1835008 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.15 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 2097152 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 53.79 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 2359296 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 57.52 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 2621440 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 56.10 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 2883584 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 54.94 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3145728 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.92 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 3407872 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 56.86 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3670016 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 51.47 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3932160 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.62 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 4194304 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.00 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 4456448 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.95 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 4718592 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.29 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 4980736 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.47 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 5242880 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.12 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 5505024 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.94 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 5767168 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 53.33 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 6029312 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.28 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 6291456 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 60.90 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 6553600 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 76.32 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 6815744 sequences have been processed. [bwa_read_seq] 1.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 66.47 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 7077888 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.52 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 7340032 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.91 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 7602176 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.34 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 7864320 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.50 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 8126464 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 51.99 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 8388608 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 55.99 sec [bwa_aln_core] write to the disk... 0.07 sec [bwa_aln_core] 8650752 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 56.83 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 8912896 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 51.26 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 9175040 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 53.97 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 9437184 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 61.14 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 9699328 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 54.08 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 9961472 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 66.22 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 10223616 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 67.23 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 10485760 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 54.24 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 10747904 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.78 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 11010048 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 60.64 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 11272192 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 55.78 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 11534336 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 56.52 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 11796480 sequences have been processed. [bwa_read_seq] 1.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.54 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 12058624 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.32 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 12320768 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.20 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 12582912 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.64 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 12845056 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 59.62 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 13107200 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.21 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 13369344 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.38 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 13631488 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 54.96 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 13893632 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.33 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 14155776 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.54 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 14417920 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.33 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 14680064 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.50 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 14942208 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.14 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 15204352 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.11 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 15466496 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 46.73 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 15728640 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.57 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 15990784 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.06 sec [bwa_aln_core] write to the disk... 0.02 sec [bwa_aln_core] 16252928 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.72 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 16515072 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.85 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 16777216 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 68.49 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 17039360 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 76.49 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 17301504 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 55.66 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 17563648 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 53.79 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 17825792 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.77 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 18087936 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 46.93 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 18350080 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 47.81 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 18612224 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.43 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 18874368 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.15 sec [bwa_aln_core] write to the disk... 0.06 sec [bwa_aln_core] 19136512 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 52.73 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 19398656 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 48.84 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 19660800 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 49.16 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 19922944 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 50.15 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 20185088 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 58.22 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 20447232 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 71.07 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 20709376 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 55.05 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 20918589 sequences have been processed. [main] Version: 0.7.13-r1126 [main] CMD: bwa aln -q 5 -l 32 -k 2 -t 1 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz [main] Real time: 4308.214 sec; CPU: 4300.200 sec | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 350 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 354 export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1" # SYS command. line 360 if [ -f "${PICARDROOT}/picard.jar" ]; then \ MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \ elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \ MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \ elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \ MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \ fi # SYS command. line 369 if [ -f "${MARKDUP}" ]; then \ java -Xmx4G -jar ${MARKDUP} \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ else \ picard MarkDuplicates \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ fi # SYS command. line 381 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 28709 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1 [Tue Nov 15 13:12:49 PST 2016] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Tue Nov 15 13:12:49 PST 2016] Executing as imk1@surya on Linux 4.4.0-45-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater INFO 2016-11-15 13:12:49 MarkDuplicates Start of doWork freeMemory: 254879344; totalMemory: 257425408; maxMemory: 11453595648 INFO 2016-11-15 13:12:49 MarkDuplicates Reading input file and constructing read end information. INFO 2016-11-15 13:12:49 MarkDuplicates Will retain up to 44052290 data points before spilling to disk. INFO 2016-11-15 13:13:06 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:13s. Time for last 1,000,000: 13s. Last read position: chr1:22,298,551 INFO 2016-11-15 13:13:06 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:13:17 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:23s. Time for last 1,000,000: 10s. Last read position: chr1:41,895,424 INFO 2016-11-15 13:13:17 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:13:27 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:34s. Time for last 1,000,000: 10s. Last read position: chr1:63,396,527 INFO 2016-11-15 13:13:27 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:13:39 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:45s. Time for last 1,000,000: 11s. Last read position: chr1:89,147,707 INFO 2016-11-15 13:13:39 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:13:46 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:53s. Time for last 1,000,000: 7s. Last read position: chr1:115,615,553 INFO 2016-11-15 13:13:46 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:13:57 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:01:04s. Time for last 1,000,000: 11s. Last read position: chr1:164,468,682 INFO 2016-11-15 13:13:57 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:14:03 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:01:10s. Time for last 1,000,000: 5s. Last read position: chr1:189,102,252 INFO 2016-11-15 13:14:03 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:14:17 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:01:23s. Time for last 1,000,000: 13s. Last read position: chr1:213,979,760 INFO 2016-11-15 13:14:17 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:14:27 MarkDuplicates Read 9,000,000 records. Elapsed time: 00:01:33s. Time for last 1,000,000: 9s. Last read position: chr1:231,814,268 INFO 2016-11-15 13:14:27 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:14:31 MarkDuplicates Read 10,000,000 records. Elapsed time: 00:01:38s. Time for last 1,000,000: 4s. Last read position: chr2:3,652,468 INFO 2016-11-15 13:14:31 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:14:42 MarkDuplicates Read 11,000,000 records. Elapsed time: 00:01:49s. Time for last 1,000,000: 11s. Last read position: chr2:39,505,122 INFO 2016-11-15 13:14:42 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:14:53 MarkDuplicates Read 12,000,000 records. Elapsed time: 00:02:00s. Time for last 1,000,000: 10s. Last read position: chr2:73,561,393 INFO 2016-11-15 13:14:53 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:14:58 MarkDuplicates Read 13,000,000 records. Elapsed time: 00:02:05s. Time for last 1,000,000: 4s. Last read position: chr2:120,526,571 INFO 2016-11-15 13:14:58 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:15:04 MarkDuplicates Read 14,000,000 records. Elapsed time: 00:02:11s. Time for last 1,000,000: 5s. Last read position: chr2:160,316,783 INFO 2016-11-15 13:15:04 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:15:12 MarkDuplicates Read 15,000,000 records. Elapsed time: 00:02:19s. Time for last 1,000,000: 8s. Last read position: chr2:195,549,735 INFO 2016-11-15 13:15:12 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:15:25 MarkDuplicates Read 16,000,000 records. Elapsed time: 00:02:31s. Time for last 1,000,000: 12s. Last read position: chr2:230,358,034 INFO 2016-11-15 13:15:25 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:15:29 MarkDuplicates Read 17,000,000 records. Elapsed time: 00:02:36s. Time for last 1,000,000: 4s. Last read position: chr3:16,305,780 INFO 2016-11-15 13:15:29 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:15:36 MarkDuplicates Read 18,000,000 records. Elapsed time: 00:02:42s. Time for last 1,000,000: 6s. Last read position: chr3:49,251,279 INFO 2016-11-15 13:15:36 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:15:43 MarkDuplicates Read 19,000,000 records. Elapsed time: 00:02:50s. Time for last 1,000,000: 7s. Last read position: chr3:84,621,225 INFO 2016-11-15 13:15:43 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:15:55 MarkDuplicates Read 20,000,000 records. Elapsed time: 00:03:02s. Time for last 1,000,000: 11s. Last read position: chr3:128,121,129 INFO 2016-11-15 13:15:55 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:16:00 MarkDuplicates Read 21,000,000 records. Elapsed time: 00:03:06s. Time for last 1,000,000: 4s. Last read position: chr3:165,373,238 INFO 2016-11-15 13:16:00 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:16:07 MarkDuplicates Read 22,000,000 records. Elapsed time: 00:03:14s. Time for last 1,000,000: 7s. Last read position: chr3:195,333,247 INFO 2016-11-15 13:16:07 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:16:15 MarkDuplicates Read 23,000,000 records. Elapsed time: 00:03:22s. Time for last 1,000,000: 7s. Last read position: chr4:37,752,582 INFO 2016-11-15 13:16:15 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:16:27 MarkDuplicates Read 24,000,000 records. Elapsed time: 00:03:34s. Time for last 1,000,000: 11s. Last read position: chr4:81,286,306 INFO 2016-11-15 13:16:27 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:16:32 MarkDuplicates Read 25,000,000 records. Elapsed time: 00:03:39s. Time for last 1,000,000: 5s. Last read position: chr4:119,339,736 INFO 2016-11-15 13:16:32 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:16:39 MarkDuplicates Read 26,000,000 records. Elapsed time: 00:03:46s. Time for last 1,000,000: 7s. Last read position: chr4:158,818,783 INFO 2016-11-15 13:16:39 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:16:45 MarkDuplicates Read 27,000,000 records. Elapsed time: 00:03:52s. Time for last 1,000,000: 6s. Last read position: chr5:6,447,551 INFO 2016-11-15 13:16:45 MarkDuplicates Tracking 16 as yet unmatched pairs. 16 records in RAM. INFO 2016-11-15 13:16:56 MarkDuplicates Read 28,000,000 records. Elapsed time: 00:04:03s. Time for last 1,000,000: 11s. Last read position: chr5:33,219,021 INFO 2016-11-15 13:16:56 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:17:00 MarkDuplicates Read 29,000,000 records. Elapsed time: 00:04:07s. Time for last 1,000,000: 3s. Last read position: chr5:60,694,078 INFO 2016-11-15 13:17:00 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:17:06 MarkDuplicates Read 30,000,000 records. Elapsed time: 00:04:12s. Time for last 1,000,000: 5s. Last read position: chr5:86,336,676 INFO 2016-11-15 13:17:06 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:17:10 MarkDuplicates Read 31,000,000 records. Elapsed time: 00:04:17s. Time for last 1,000,000: 4s. Last read position: chr5:112,930,184 INFO 2016-11-15 13:17:10 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:17:15 MarkDuplicates Read 32,000,000 records. Elapsed time: 00:04:21s. Time for last 1,000,000: 4s. Last read position: chr5:136,852,889 INFO 2016-11-15 13:17:15 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:17:18 MarkDuplicates Read 33,000,000 records. Elapsed time: 00:04:25s. Time for last 1,000,000: 3s. Last read position: chr5:160,271,193 INFO 2016-11-15 13:17:18 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:17:28 MarkDuplicates Read 34,000,000 records. Elapsed time: 00:04:35s. Time for last 1,000,000: 9s. Last read position: chr6:4,126,386 INFO 2016-11-15 13:17:28 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:17:32 MarkDuplicates Read 35,000,000 records. Elapsed time: 00:04:39s. Time for last 1,000,000: 4s. Last read position: chr6:28,666,579 INFO 2016-11-15 13:17:32 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:17:36 MarkDuplicates Read 36,000,000 records. Elapsed time: 00:04:43s. Time for last 1,000,000: 3s. Last read position: chr6:49,527,281 INFO 2016-11-15 13:17:36 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:17:41 MarkDuplicates Read 37,000,000 records. Elapsed time: 00:04:48s. Time for last 1,000,000: 5s. Last read position: chr6:81,728,195 INFO 2016-11-15 13:17:41 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:17:47 MarkDuplicates Read 38,000,000 records. Elapsed time: 00:04:54s. Time for last 1,000,000: 5s. Last read position: chr6:107,535,760 INFO 2016-11-15 13:17:47 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:18:04 MarkDuplicates Read 39,000,000 records. Elapsed time: 00:05:10s. Time for last 1,000,000: 16s. Last read position: chr6:132,364,408 INFO 2016-11-15 13:18:04 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:18:09 MarkDuplicates Read 40,000,000 records. Elapsed time: 00:05:15s. Time for last 1,000,000: 5s. Last read position: chr6:156,918,595 INFO 2016-11-15 13:18:09 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:18:15 MarkDuplicates Read 41,000,000 records. Elapsed time: 00:05:22s. Time for last 1,000,000: 6s. Last read position: chr7:18,636,743 INFO 2016-11-15 13:18:15 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:18:23 MarkDuplicates Read 42,000,000 records. Elapsed time: 00:05:29s. Time for last 1,000,000: 7s. Last read position: chr7:53,850,313 INFO 2016-11-15 13:18:23 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:18:29 MarkDuplicates Read 43,000,000 records. Elapsed time: 00:05:36s. Time for last 1,000,000: 6s. Last read position: chr7:96,503,433 INFO 2016-11-15 13:18:29 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:18:34 MarkDuplicates Read 44,000,000 records. Elapsed time: 00:05:41s. Time for last 1,000,000: 4s. Last read position: chr7:120,651,846 INFO 2016-11-15 13:18:34 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:19:19 MarkDuplicates Read 45,000,000 records. Elapsed time: 00:06:25s. Time for last 1,000,000: 44s. Last read position: chr7:146,401,497 INFO 2016-11-15 13:19:19 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:19:24 MarkDuplicates Read 46,000,000 records. Elapsed time: 00:06:30s. Time for last 1,000,000: 5s. Last read position: chr8:17,030,519 INFO 2016-11-15 13:19:24 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:19:32 MarkDuplicates Read 47,000,000 records. Elapsed time: 00:06:39s. Time for last 1,000,000: 8s. Last read position: chr8:42,750,671 INFO 2016-11-15 13:19:32 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:19:36 MarkDuplicates Read 48,000,000 records. Elapsed time: 00:06:43s. Time for last 1,000,000: 3s. Last read position: chr8:85,385,487 INFO 2016-11-15 13:19:36 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:19:41 MarkDuplicates Read 49,000,000 records. Elapsed time: 00:06:47s. Time for last 1,000,000: 4s. Last read position: chr8:124,358,985 INFO 2016-11-15 13:19:41 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:19:46 MarkDuplicates Read 50,000,000 records. Elapsed time: 00:06:53s. Time for last 1,000,000: 5s. Last read position: chr9:18,183,883 INFO 2016-11-15 13:19:46 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:19:51 MarkDuplicates Read 51,000,000 records. Elapsed time: 00:06:58s. Time for last 1,000,000: 5s. Last read position: chr9:84,163,983 INFO 2016-11-15 13:19:51 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:19:55 MarkDuplicates Read 52,000,000 records. Elapsed time: 00:07:02s. Time for last 1,000,000: 4s. Last read position: chr9:119,875,535 INFO 2016-11-15 13:19:55 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:20:01 MarkDuplicates Read 53,000,000 records. Elapsed time: 00:07:08s. Time for last 1,000,000: 5s. Last read position: chr10:5,661,683 INFO 2016-11-15 13:20:01 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:20:06 MarkDuplicates Read 54,000,000 records. Elapsed time: 00:07:13s. Time for last 1,000,000: 5s. Last read position: chr10:29,748,948 INFO 2016-11-15 13:20:06 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:20:15 MarkDuplicates Read 55,000,000 records. Elapsed time: 00:07:22s. Time for last 1,000,000: 9s. Last read position: chr10:61,280,221 INFO 2016-11-15 13:20:15 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:20:20 MarkDuplicates Read 56,000,000 records. Elapsed time: 00:07:27s. Time for last 1,000,000: 5s. Last read position: chr10:85,305,552 INFO 2016-11-15 13:20:20 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:20:24 MarkDuplicates Read 57,000,000 records. Elapsed time: 00:07:31s. Time for last 1,000,000: 3s. Last read position: chr10:108,129,202 INFO 2016-11-15 13:20:24 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:20:28 MarkDuplicates Read 58,000,000 records. Elapsed time: 00:07:35s. Time for last 1,000,000: 4s. Last read position: chr10:132,730,123 INFO 2016-11-15 13:20:28 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:20:33 MarkDuplicates Read 59,000,000 records. Elapsed time: 00:07:40s. Time for last 1,000,000: 4s. Last read position: chr11:35,359,267 INFO 2016-11-15 13:20:33 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:20:38 MarkDuplicates Read 60,000,000 records. Elapsed time: 00:07:45s. Time for last 1,000,000: 4s. Last read position: chr11:75,696,431 INFO 2016-11-15 13:20:38 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:20:48 MarkDuplicates Read 61,000,000 records. Elapsed time: 00:07:54s. Time for last 1,000,000: 9s. Last read position: chr11:114,414,621 INFO 2016-11-15 13:20:48 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:20:51 MarkDuplicates Read 62,000,000 records. Elapsed time: 00:07:58s. Time for last 1,000,000: 3s. Last read position: chr12:8,900,276 INFO 2016-11-15 13:20:51 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:20:56 MarkDuplicates Read 63,000,000 records. Elapsed time: 00:08:03s. Time for last 1,000,000: 4s. Last read position: chr12:38,863,889 INFO 2016-11-15 13:20:56 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:21:01 MarkDuplicates Read 64,000,000 records. Elapsed time: 00:08:08s. Time for last 1,000,000: 5s. Last read position: chr12:60,598,824 INFO 2016-11-15 13:21:01 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:21:07 MarkDuplicates Read 65,000,000 records. Elapsed time: 00:08:14s. Time for last 1,000,000: 5s. Last read position: chr12:88,520,538 INFO 2016-11-15 13:21:07 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:21:12 MarkDuplicates Read 66,000,000 records. Elapsed time: 00:08:19s. Time for last 1,000,000: 5s. Last read position: chr12:112,268,437 INFO 2016-11-15 13:21:12 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:21:18 MarkDuplicates Read 67,000,000 records. Elapsed time: 00:08:25s. Time for last 1,000,000: 6s. Last read position: chr13:23,999,632 INFO 2016-11-15 13:21:18 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:21:25 MarkDuplicates Read 68,000,000 records. Elapsed time: 00:08:31s. Time for last 1,000,000: 6s. Last read position: chr13:51,165,870 INFO 2016-11-15 13:21:25 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:21:40 MarkDuplicates Read 69,000,000 records. Elapsed time: 00:08:47s. Time for last 1,000,000: 15s. Last read position: chr13:88,986,369 INFO 2016-11-15 13:21:40 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:21:46 MarkDuplicates Read 70,000,000 records. Elapsed time: 00:08:53s. Time for last 1,000,000: 5s. Last read position: chr14:23,149,367 INFO 2016-11-15 13:21:46 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:21:51 MarkDuplicates Read 71,000,000 records. Elapsed time: 00:08:57s. Time for last 1,000,000: 4s. Last read position: chr14:58,140,905 INFO 2016-11-15 13:21:51 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:21:56 MarkDuplicates Read 72,000,000 records. Elapsed time: 00:09:03s. Time for last 1,000,000: 5s. Last read position: chr14:91,912,553 INFO 2016-11-15 13:21:56 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:22:01 MarkDuplicates Read 73,000,000 records. Elapsed time: 00:09:08s. Time for last 1,000,000: 5s. Last read position: chr15:38,895,095 INFO 2016-11-15 13:22:01 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:22:06 MarkDuplicates Read 74,000,000 records. Elapsed time: 00:09:13s. Time for last 1,000,000: 5s. Last read position: chr15:68,188,367 INFO 2016-11-15 13:22:06 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:22:11 MarkDuplicates Read 75,000,000 records. Elapsed time: 00:09:17s. Time for last 1,000,000: 4s. Last read position: chr15:87,921,272 INFO 2016-11-15 13:22:11 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:22:15 MarkDuplicates Read 76,000,000 records. Elapsed time: 00:09:21s. Time for last 1,000,000: 4s. Last read position: chr16:11,143,813 INFO 2016-11-15 13:22:15 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:22:26 MarkDuplicates Read 77,000,000 records. Elapsed time: 00:09:33s. Time for last 1,000,000: 11s. Last read position: chr16:51,391,437 INFO 2016-11-15 13:22:26 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:22:30 MarkDuplicates Read 78,000,000 records. Elapsed time: 00:09:37s. Time for last 1,000,000: 4s. Last read position: chr16:84,928,458 INFO 2016-11-15 13:22:30 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:22:34 MarkDuplicates Read 79,000,000 records. Elapsed time: 00:09:41s. Time for last 1,000,000: 4s. Last read position: chr17:18,368,935 INFO 2016-11-15 13:22:34 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:22:39 MarkDuplicates Read 80,000,000 records. Elapsed time: 00:09:46s. Time for last 1,000,000: 4s. Last read position: chr17:44,246,522 INFO 2016-11-15 13:22:39 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:22:43 MarkDuplicates Read 81,000,000 records. Elapsed time: 00:09:50s. Time for last 1,000,000: 3s. Last read position: chr17:66,172,158 INFO 2016-11-15 13:22:43 MarkDuplicates Tracking 10 as yet unmatched pairs. 10 records in RAM. INFO 2016-11-15 13:22:47 MarkDuplicates Read 82,000,000 records. Elapsed time: 00:09:54s. Time for last 1,000,000: 4s. Last read position: chr18:5,619,614 INFO 2016-11-15 13:22:47 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:22:52 MarkDuplicates Read 83,000,000 records. Elapsed time: 00:09:59s. Time for last 1,000,000: 4s. Last read position: chr18:46,152,631 INFO 2016-11-15 13:22:52 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:22:56 MarkDuplicates Read 84,000,000 records. Elapsed time: 00:10:03s. Time for last 1,000,000: 4s. Last read position: chr19:1,328,731 INFO 2016-11-15 13:22:56 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:23:01 MarkDuplicates Read 85,000,000 records. Elapsed time: 00:10:07s. Time for last 1,000,000: 4s. Last read position: chr19:28,148,444 INFO 2016-11-15 13:23:01 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:23:13 MarkDuplicates Read 86,000,000 records. Elapsed time: 00:10:20s. Time for last 1,000,000: 12s. Last read position: chr19:40,341,579 INFO 2016-11-15 13:23:13 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:23:18 MarkDuplicates Read 87,000,000 records. Elapsed time: 00:10:24s. Time for last 1,000,000: 4s. Last read position: chr20:3,222,035 INFO 2016-11-15 13:23:18 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:23:23 MarkDuplicates Read 88,000,000 records. Elapsed time: 00:10:30s. Time for last 1,000,000: 5s. Last read position: chr20:37,218,903 INFO 2016-11-15 13:23:23 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:23:56 MarkDuplicates Read 89,000,000 records. Elapsed time: 00:11:03s. Time for last 1,000,000: 33s. Last read position: chr20:62,871,367 INFO 2016-11-15 13:23:56 MarkDuplicates Tracking 10 as yet unmatched pairs. 10 records in RAM. INFO 2016-11-15 13:24:01 MarkDuplicates Read 90,000,000 records. Elapsed time: 00:11:08s. Time for last 1,000,000: 4s. Last read position: chr21:39,474,524 INFO 2016-11-15 13:24:01 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:24:05 MarkDuplicates Read 91,000,000 records. Elapsed time: 00:11:12s. Time for last 1,000,000: 4s. Last read position: chr22:31,346,944 INFO 2016-11-15 13:24:05 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-15 13:24:10 MarkDuplicates Read 92,000,000 records. Elapsed time: 00:11:16s. Time for last 1,000,000: 4s. Last read position: chrX:6,235,951 INFO 2016-11-15 13:24:10 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:24:14 MarkDuplicates Read 93,000,000 records. Elapsed time: 00:11:21s. Time for last 1,000,000: 4s. Last read position: chrX:38,820,975 INFO 2016-11-15 13:24:14 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-15 13:24:19 MarkDuplicates Read 94,000,000 records. Elapsed time: 00:11:26s. Time for last 1,000,000: 5s. Last read position: chrX:69,473,954 INFO 2016-11-15 13:24:19 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:24:24 MarkDuplicates Read 95,000,000 records. Elapsed time: 00:11:31s. Time for last 1,000,000: 4s. Last read position: chrX:92,683,143 INFO 2016-11-15 13:24:24 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-15 13:24:27 MarkDuplicates Read 96,000,000 records. Elapsed time: 00:11:34s. Time for last 1,000,000: 3s. Last read position: chrX:118,447,518 INFO 2016-11-15 13:24:27 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-15 13:24:35 MarkDuplicates Read 97,000,000 records. Elapsed time: 00:11:42s. Time for last 1,000,000: 7s. Last read position: chrX:142,819,613 INFO 2016-11-15 13:24:35 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 13:24:41 MarkDuplicates Read 98,000,000 records. Elapsed time: 00:11:48s. Time for last 1,000,000: 6s. Last read position: chrM:3,995 INFO 2016-11-15 13:24:41 MarkDuplicates Tracking 7472 as yet unmatched pairs. 7472 records in RAM. INFO 2016-11-15 13:24:51 MarkDuplicates Read 99,000,000 records. Elapsed time: 00:11:57s. Time for last 1,000,000: 9s. Last read position: chrM:11,775 INFO 2016-11-15 13:24:51 MarkDuplicates Tracking 14000 as yet unmatched pairs. 14000 records in RAM. INFO 2016-11-15 13:24:54 MarkDuplicates Read 99989486 records. 0 pairs never matched. INFO 2016-11-15 13:25:00 MarkDuplicates After buildSortedReadEndLists freeMemory: 5646605608; totalMemory: 5696913408; maxMemory: 11453595648 INFO 2016-11-15 13:25:00 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk. INFO 2016-11-15 13:25:01 MarkDuplicates Traversing read pair information and detecting duplicates. INFO 2016-11-15 13:25:14 MarkDuplicates Traversing fragment information and detecting duplicates. INFO 2016-11-15 13:25:37 MarkDuplicates Sorting list of duplicate records. INFO 2016-11-15 13:25:38 MarkDuplicates After generateDuplicateIndexes freeMemory: 5683994040; totalMemory: 8602517504; maxMemory: 11453595648 INFO 2016-11-15 13:25:38 MarkDuplicates Marking 496224 records as duplicates. INFO 2016-11-15 13:25:38 MarkDuplicates Found 42648 optical duplicate clusters. INFO 2016-11-15 13:27:52 MarkDuplicates Written 10,000,000 records. Elapsed time: 00:02:13s. Time for last 10,000,000: 133s. Last read position: chr2:3,652,468 INFO 2016-11-15 13:30:03 MarkDuplicates Written 20,000,000 records. Elapsed time: 00:04:25s. Time for last 10,000,000: 131s. Last read position: chr3:128,121,129 INFO 2016-11-15 13:32:13 MarkDuplicates Written 30,000,000 records. Elapsed time: 00:06:35s. Time for last 10,000,000: 129s. Last read position: chr5:86,336,676 INFO 2016-11-15 13:34:21 MarkDuplicates Written 40,000,000 records. Elapsed time: 00:08:43s. Time for last 10,000,000: 128s. Last read position: chr6:156,918,595 INFO 2016-11-15 13:36:29 MarkDuplicates Written 50,000,000 records. Elapsed time: 00:10:51s. Time for last 10,000,000: 127s. Last read position: chr9:18,183,883 INFO 2016-11-15 13:38:50 MarkDuplicates Written 60,000,000 records. Elapsed time: 00:13:12s. Time for last 10,000,000: 140s. Last read position: chr11:75,696,431 INFO 2016-11-15 13:41:07 MarkDuplicates Written 70,000,000 records. Elapsed time: 00:15:29s. Time for last 10,000,000: 137s. Last read position: chr14:23,149,367 INFO 2016-11-15 13:43:28 MarkDuplicates Written 80,000,000 records. Elapsed time: 00:17:50s. Time for last 10,000,000: 140s. Last read position: chr17:44,246,522 INFO 2016-11-15 13:46:06 MarkDuplicates Written 90,000,000 records. Elapsed time: 00:20:28s. Time for last 10,000,000: 158s. Last read position: chr21:39,474,524 INFO 2016-11-15 13:48:46 MarkDuplicates Before output close freeMemory: 8414838376; totalMemory: 8482455552; maxMemory: 11453595648 INFO 2016-11-15 13:48:46 MarkDuplicates After output close freeMemory: 8413788744; totalMemory: 8481406976; maxMemory: 11453595648 [Tue Nov 15 13:48:46 PST 2016] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 35.95 minutes. Runtime.totalMemory()=8481406976 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 146 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 150 samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam # SYS command. line 153 sambamba index -t 4 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam # SYS command. line 155 sambamba flagstat -t 4 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/ctl1/ss_50M_2000_GRCh38.nodup.flagstat.qc # SYS command. line 165 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam | \ awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \ grep -v 'chrM' | sort | uniq -c | \ awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/ctl1/ss_50M_2000_GRCh38.nodup.pbc.qc # SYS command. line 169 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 33011 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 142 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 147 bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz | samtools view -Su /dev/stdin \ | sambamba sort -t 1 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.bam # SYS command. line 149 sambamba flagstat -t 1 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.flagstat.qc # SYS command. line 151 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 45179 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 67.28 sec [bwa_aln_core] refine gapped alignments... 0.64 sec [bwa_aln_core] print alignments... [samopen] SAM header is present: 493 sequences. 0.47 sec [bwa_aln_core] 262144 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 66.81 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 524288 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 62.16 sec [bwa_aln_core] refine gapped alignments... 0.52 sec [bwa_aln_core] print alignments... 0.62 sec [bwa_aln_core] 786432 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 63.67 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 1048576 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 61.21 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 1310720 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 64.58 sec [bwa_aln_core] refine gapped alignments... 0.53 sec [bwa_aln_core] print alignments... 0.41 sec [bwa_aln_core] 1572864 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 67.15 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 1835008 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 75.29 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 2097152 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 88.55 sec [bwa_aln_core] refine gapped alignments... 2.18 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 2359296 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 105.08 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 2621440 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 37.86 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 2883584 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 16.55 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.66 sec [bwa_aln_core] 3145728 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 14.96 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 3407872 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 17.78 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 3670016 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 19.37 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 3932160 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 21.38 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 4194304 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 22.00 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 4456448 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 24.44 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 4718592 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 25.35 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 4980736 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 26.89 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 5242880 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.94 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.64 sec [bwa_aln_core] 5505024 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.69 sec [bwa_aln_core] refine gapped alignments... 0.53 sec [bwa_aln_core] print alignments... 0.46 sec [bwa_aln_core] 5767168 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 30.25 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 6029312 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 30.68 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 6291456 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 33.47 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 6553600 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 33.20 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.53 sec [bwa_aln_core] 6815744 sequences have been processed. [bwa_read_seq] 1.1% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 35.42 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 7077888 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 36.90 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 7340032 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 38.75 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 7602176 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 39.76 sec [bwa_aln_core] refine gapped alignments... 0.62 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 7864320 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 40.74 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 8126464 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 44.07 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 8388608 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 46.43 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 8650752 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 49.99 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.57 sec [bwa_aln_core] 8912896 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 52.60 sec [bwa_aln_core] refine gapped alignments... 0.59 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 9175040 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 52.19 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 9437184 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 51.54 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 9699328 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 51.47 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 9961472 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 53.84 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.60 sec [bwa_aln_core] 10223616 sequences have been processed. [bwa_read_seq] 1.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 49.28 sec [bwa_aln_core] refine gapped alignments... 0.57 sec [bwa_aln_core] print alignments... 0.59 sec [bwa_aln_core] 10485760 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 36.03 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 10747904 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 34.13 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 11010048 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 35.60 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 11272192 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.54 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.58 sec [bwa_aln_core] 11534336 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 30.45 sec [bwa_aln_core] refine gapped alignments... 0.51 sec [bwa_aln_core] print alignments... 0.54 sec [bwa_aln_core] 11796480 sequences have been processed. [bwa_read_seq] 1.0% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.31 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 12058624 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.46 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 12320768 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.87 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.52 sec [bwa_aln_core] 12582912 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.92 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 12845056 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 30.55 sec [bwa_aln_core] refine gapped alignments... 0.50 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 13107200 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.99 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 13369344 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.42 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 13631488 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.46 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 13893632 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.77 sec [bwa_aln_core] refine gapped alignments... 0.51 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 14155776 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.30 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.50 sec [bwa_aln_core] 14417920 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.61 sec [bwa_aln_core] refine gapped alignments... 0.52 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 14680064 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.21 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.42 sec [bwa_aln_core] 14942208 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 26.61 sec [bwa_aln_core] refine gapped alignments... 0.61 sec [bwa_aln_core] print alignments... 0.42 sec [bwa_aln_core] 15204352 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.51 sec [bwa_aln_core] refine gapped alignments... 0.54 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 15466496 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.41 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.49 sec [bwa_aln_core] 15728640 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.53 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 15990784 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.38 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.44 sec [bwa_aln_core] 16252928 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.16 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.56 sec [bwa_aln_core] 16515072 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.75 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 16777216 sequences have been processed. [bwa_read_seq] 1.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.30 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.42 sec [bwa_aln_core] 17039360 sequences have been processed. [bwa_read_seq] 2.3% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.31 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.45 sec [bwa_aln_core] 17301504 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.30 sec [bwa_aln_core] refine gapped alignments... 0.47 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 17563648 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.70 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.39 sec [bwa_aln_core] 17825792 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.93 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 18087936 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.63 sec [bwa_aln_core] refine gapped alignments... 0.60 sec [bwa_aln_core] print alignments... 0.40 sec [bwa_aln_core] 18350080 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 28.92 sec [bwa_aln_core] refine gapped alignments... 0.55 sec [bwa_aln_core] print alignments... 0.55 sec [bwa_aln_core] 18612224 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.04 sec [bwa_aln_core] refine gapped alignments... 0.52 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 18874368 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 27.91 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 19136512 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 25.10 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.47 sec [bwa_aln_core] 19398656 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 24.79 sec [bwa_aln_core] refine gapped alignments... 0.58 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 19660800 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 24.11 sec [bwa_aln_core] refine gapped alignments... 0.53 sec [bwa_aln_core] print alignments... 0.48 sec [bwa_aln_core] 19922944 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 23.34 sec [bwa_aln_core] refine gapped alignments... 0.51 sec [bwa_aln_core] print alignments... 0.43 sec [bwa_aln_core] 20185088 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 29.13 sec [bwa_aln_core] refine gapped alignments... 0.56 sec [bwa_aln_core] print alignments... 0.51 sec [bwa_aln_core] 20447232 sequences have been processed. [bwa_read_seq] 1.7% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 26.15 sec [bwa_aln_core] refine gapped alignments... 0.49 sec [bwa_aln_core] print alignments... 0.46 sec [bwa_aln_core] 20709376 sequences have been processed. [bwa_read_seq] 1.4% bases are trimmed. [bwa_aln_core] convert to sequence coordinate... 25.46 sec [bwa_aln_core] refine gapped alignments... 0.48 sec [bwa_aln_core] print alignments... 0.32 sec [bwa_aln_core] 20918589 sequences have been processed. [main] Version: 0.7.13-r1126 [main] CMD: bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz [main] Real time: 3134.957 sec; CPU: 3045.648 sec | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 507 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 510 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/ctl1/ss_50M_2000_GRCh38.nodup.tagAlign.gz # SYS command. line 512 echo # SYS command. line 514 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 42096 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 97 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 105 if [[ 0 > 0 ]]; then \ sambamba sort -t 1 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.qnmsrt.bam; \ samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \ samtools view -F 1804 -Su /dev/stdin | \ sambamba sort -t 1 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.filt.bam; \ rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.qnmsrt.bam; \ else \ samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.bam | \ sambamba sort -t 1 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.filt.bam; \ fi # SYS command. line 116 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43236 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 350 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 354 export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1" # SYS command. line 360 if [ -f "${PICARDROOT}/picard.jar" ]; then \ MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \ elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \ MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \ elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \ MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \ fi # SYS command. line 369 if [ -f "${MARKDUP}" ]; then \ java -Xmx4G -jar ${MARKDUP} \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ else \ picard MarkDuplicates \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ fi # SYS command. line 381 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 31980 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1 [Tue Nov 15 15:03:35 PST 2016] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Tue Nov 15 15:03:35 PST 2016] Executing as imk1@kadru on Linux 4.4.0-22-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater INFO 2016-11-15 15:03:35 MarkDuplicates Start of doWork freeMemory: 254879328; totalMemory: 257425408; maxMemory: 11453595648 INFO 2016-11-15 15:03:35 MarkDuplicates Reading input file and constructing read end information. INFO 2016-11-15 15:03:35 MarkDuplicates Will retain up to 44052290 data points before spilling to disk. INFO 2016-11-15 15:03:43 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:07s. Time for last 1,000,000: 7s. Last read position: chr1:201,731,280 INFO 2016-11-15 15:03:43 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:03:49 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:12s. Time for last 1,000,000: 5s. Last read position: chr2:185,479,193 INFO 2016-11-15 15:03:49 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:03:53 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:16s. Time for last 1,000,000: 3s. Last read position: chr3:188,351,050 INFO 2016-11-15 15:03:53 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:00 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:23s. Time for last 1,000,000: 7s. Last read position: chr5:43,157,596 INFO 2016-11-15 15:04:00 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:05 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:28s. Time for last 1,000,000: 4s. Last read position: chr6:46,294,643 INFO 2016-11-15 15:04:05 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:12 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:00:35s. Time for last 1,000,000: 7s. Last read position: chr7:94,376,438 INFO 2016-11-15 15:04:12 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:17 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:00:41s. Time for last 1,000,000: 5s. Last read position: chr9:12,819,163 INFO 2016-11-15 15:04:17 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:20 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:00:43s. Time for last 1,000,000: 2s. Last read position: chr10:110,042,780 INFO 2016-11-15 15:04:20 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:28 MarkDuplicates Read 9,000,000 records. Elapsed time: 00:00:51s. Time for last 1,000,000: 8s. Last read position: chr12:67,131,090 INFO 2016-11-15 15:04:28 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:32 MarkDuplicates Read 10,000,000 records. Elapsed time: 00:00:55s. Time for last 1,000,000: 3s. Last read position: chr14:57,699,012 INFO 2016-11-15 15:04:32 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:37 MarkDuplicates Read 11,000,000 records. Elapsed time: 00:01:01s. Time for last 1,000,000: 5s. Last read position: chr17:14,101,230 INFO 2016-11-15 15:04:37 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:45 MarkDuplicates Read 12,000,000 records. Elapsed time: 00:01:08s. Time for last 1,000,000: 7s. Last read position: chr19:39,638,108 INFO 2016-11-15 15:04:45 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:51 MarkDuplicates Read 13,000,000 records. Elapsed time: 00:01:15s. Time for last 1,000,000: 6s. Last read position: chrX:30,711,881 INFO 2016-11-15 15:04:51 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-15 15:04:53 MarkDuplicates Read 13854582 records. 0 pairs never matched. INFO 2016-11-15 15:04:57 MarkDuplicates After buildSortedReadEndLists freeMemory: 4263232704; totalMemory: 5584191488; maxMemory: 11453595648 INFO 2016-11-15 15:04:57 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk. INFO 2016-11-15 15:04:58 MarkDuplicates Traversing read pair information and detecting duplicates. INFO 2016-11-15 15:04:58 MarkDuplicates Traversing fragment information and detecting duplicates. INFO 2016-11-15 15:04:58 MarkDuplicates Sorting list of duplicate records. INFO 2016-11-15 15:05:28 MarkDuplicates After generateDuplicateIndexes freeMemory: 5505226400; totalMemory: 8449425408; maxMemory: 11453595648 INFO 2016-11-15 15:05:28 MarkDuplicates Marking 11949789 records as duplicates. INFO 2016-11-15 15:05:28 MarkDuplicates Found 0 optical duplicate clusters. INFO 2016-11-15 15:06:40 MarkDuplicates Written 10,000,000 records. Elapsed time: 00:01:12s. Time for last 10,000,000: 72s. Last read position: chr14:57,699,012 INFO 2016-11-15 15:07:06 MarkDuplicates Before output close freeMemory: 8247243992; totalMemory: 8329887744; maxMemory: 11453595648 INFO 2016-11-15 15:07:08 MarkDuplicates After output close freeMemory: 8355770448; totalMemory: 8438415360; maxMemory: 11453595648 [Tue Nov 15 15:07:08 PST 2016] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 3.54 minutes. Runtime.totalMemory()=8438415360 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 146 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 150 samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.bam # SYS command. line 153 sambamba index -t 1 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.bam # SYS command. line 155 sambamba flagstat -t 1 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.flagstat.qc # SYS command. line 165 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.dupmark.bam | \ awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \ grep -v 'chrM' | sort | uniq -c | \ awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.pbc.qc # SYS command. line 169 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43554 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 507 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 510 bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz # SYS command. line 512 echo # SYS command. line 514 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43713 (process ID) old priority 0, new priority 10 Waiting for 55 seconds. | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 47 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 50 zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz | \ grep -v "chrM" | shuf -n 15000000 --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.15M.tagAlign.gz # SYS command. line 53 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43805 (process ID) old priority 0, new priority 10 Waiting for 57 seconds. | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 257 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 260 nlines=$( zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz | wc -l ) # SYS command. line 261 nlines=$(( (nlines + 1) / 2 )) # SYS command. line 265 zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz | shuf --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.tagAlign.gz | split -d -l $((nlines)) - /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup. # SYS command. line 268 gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup.00 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup.pr1.tagAlign.gz # SYS command. line 269 rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup.00 # SYS command. line 270 gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup.01 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr2/SRR1370903_1.nodup.pr2.tagAlign.gz # SYS command. line 271 rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/pseudo_reps/rep1/pr1/SRR1370903_1.nodup.01 # SYS command. line 273 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43806 (process ID) old priority 0, new priority 10 Waiting for 55 seconds. | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 214 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 217 if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R); \ else RUN_SPP=$(which run_spp.R); \ fi # SYS command. line 223 Rscript ${RUN_SPP} -rf \ -c=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.15M.tagAlign.gz -p=1 \ -filtchr=chrM -savp=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.plot.pdf -out=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc # SYS command. line 226 sed -r 's/,[^\t]+//g' /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc.tmp # SYS command. line 227 mv /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc.tmp /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc # SYS command. line 229 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 43960 (process ID) old priority 0, new priority 10 ################ ChIP data: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.15M.tagAlign.gz Control data: NA strandshift(min): -500 strandshift(step): 5 strandshift(max) 1500 user-defined peak shift NA exclusion(min): 10 exclusion(max): NaN num parallel nodes: 1 FDR threshold: 0.01 NumPeaks Threshold: NA Output Directory: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1 narrowPeak output file name: NA regionPeak output file name: NA Rdata filename: NA plot pdf filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.plot.pdf result filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.qc Overwrite files?: TRUE Decompressing ChIP file Reading ChIP tagAlign/BAM file /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/align/rep1/SRR1370903_1.nodup.15M.tagAlign.gz opened /tmp/12358.1.q/RtmpwW4kVF/SRR1370903_1.nodup.15M.tagAlignabf935c4911b done. read 1904233 fragments ChIP data read length 51 [1] TRUE Calculating peak characteristics Minimum cross-correlation value 0.02623488 Minimum cross-correlation shift 1500 Top 3 cross-correlation values 0.0272133685816513,0.0269441206570071,0.0269338797262606 Top 3 estimates for fragment length 85,160,170 Window half size 330 Phantom peak location 50 Phantom peak Correlation 0.02776752 Normalized Strand cross-correlation coefficient (NSC) 1.037297 Relative Strand cross-correlation Coefficient (RSC) 0.6384345 Phantom Peak Quality Tag 0 null device 1 --------------------Stderr-------------------- Loading required package: caTools Warning message: package ‘caTools’ was built under R version 3.2.3 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 98 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 99 dot -Tsvg /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/report/SRR1370903_1_workflow.dot > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/report/SRR1370903_1_workflow.svg 2> /dev/null || echo "svg: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/report/SRR1370903_1_workflow.svg" # to suppress dot font error (exit code=1) --------------------Stdout-------------------- 44071 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 325 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 326 gs -dFirstPage=1 -dLastPage=1 -dTextAlphaBits=4 -dGraphicsAlphaBits=4 -r110x110 \ -dUseCropBox -dQUIET -dSAFER -dBATCH -dNOPAUSE -dNOPROMPT -sDEVICE=png16m \ -sOutputFile=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.plot.png \ -r144 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out/qc/rep1/SRR1370903_1.nodup.15M.cc.plot.pdf --------------------Stdout-------------------- 44107 (process ID) old priority 0, new priority 10 |
Type | Name | Value |
---|---|---|
string | _ | /usr/bin/bds |
string | _LMFILES_ | /software/modulefiles/modsappsdir/modsappsdir:/software/modulefiles/sratoolkit/2.5.2:/software/modulefiles/ascp/3.5.6 |
bool | allowEmpty | false |
string[] | args | [-title, SRR1370903_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370903_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
string | ASCPKEY | /software/ascp/etc/asperaweb_id_dsa.openssh |
bool | canFail | false |
int | cpus | -1 |
int | cpusLocal | 56 |
int | day | 86400 |
string | DEEPLIFT_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/deeplift_private/deeplift |
string | DERBY_HOME | /usr/lib/jvm/java-8-oracle/db |
string | DISPLAY | localhost:12.0 |
real | E | 2.718281828459045 |
string | ENHANCER_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/enhancer_prediction_code/ |
int | G | 1073741824 |
string | HOME | /users/imk1/ |
int | hour | 3600 |
string | J2REDIR | /usr/lib/jvm/java-8-oracle/jre |
string | J2SDKDIR | /usr/lib/jvm/java-8-oracle |
string | JAVA_HOME | /usr/lib/jvm/java-8-oracle |
int | K | 1024 |
string | KERAS_DIR | /users/imk1/.local/lib/python2.7/site-packages/keras/ |
string | KRB5CCNAME | FILE:/tmp/krb5cc_1048_N5SeQf |
string | LANG | en_US.UTF-8 |
string | LOADEDMODULES | modsappsdir/modsappsdir:sratoolkit/2.5.2:ascp/3.5.6 |
string | LOGNAME | imk1 |
int | M | 1048576 |
string | /var/mail/imk1 | |
int | mem | -1 |
int | minute | 60 |
string | MODULE_VERSION | 3.2.10 |
string | MODULE_VERSION_STACK | 3.2.10 |
string | MODULEPATH | /usr/local/Modules/versions\t\t\t\t:/usr/local/Modules/$MODULE_VERSION/modulefiles\t:/modules/\t\t\t\t:/software/modulefiles |
string | MODULESAPPSDIR | /software |
string | MODULESHOME | /software/env_module/3.2.10 |
string | NLSPATH | /usr/dt/lib/nls/msg/%L/%N.cat |
string | node | |
int | P | 1125899906842624 |
string | PATH | /users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/software/ascp/:/software/sratoolkit/2.5.2/bin/:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/software/miniconda3/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect:/users/imk1/edirect |
string | PERL5LIB | /users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5:/users/imk1/perl5/lib/perl5 |
string | PERL_LOCAL_LIB_ROOT | /users/imk1/perl5:/users/imk1/perl5:/users/imk1/perl5:/users/imk1/perl5 |
string | PERL_MB_OPT | --install_base \"/users/imk1/perl5\" |
string | PERL_MM_OPT | INSTALL_BASE=/users/imk1/perl5 |
real | PI | 3.141592653589793 |
string | ppwd | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1 |
string | programName | chipseq.bds |
string | programPath | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/chipseq.bds |
string | PWD | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370903_1 |
string | PYTHONPATH | /users/imk1/.local/lib/python2.7/site-packages/keras/:/srv/scratch/imk1/TFBindingPredictionProject/src/pybedtools/ |
string | queue | |
int | retry | 0 |
string | RULEFITBASE | /srv/scratch/imk1/TFBindingPredictionProject/src/RuleFit/ |
string | SHELL | /bin/bash |
string | SHLVL | 3 |
string | SSH_CLIENT | 171.65.77.8 55144 22 |
string | SSH_CONNECTION | 171.65.77.8 55144 171.65.76.63 22 |
string | SSH_TTY | /dev/pts/4 |
string | STY | 16029.SRR1370903_1.BDS |
string | system | sge |
int | T | 1099511627776 |
string | TERM | screen |
string | TERMCAP | SC|screen|VT 100/ANSI X3.64 virtual terminal:\\\n\t:DO=\\E[%dB:LE=\\E[%dD:RI=\\E[%dC:UP=\\E[%dA:bs:bt=\\E[Z:\\\n\t:cd=\\E[J:ce=\\E[K:cl=\\E[H\\E[J:cm=\\E[%i%d;%dH:ct=\\E[3g:\\\n\t:do=^J:nd=\\E[C:pt:rc=\\E8:rs=\\Ec:sc=\\E7:st=\\EH:up=\\EM:\\\n\t:le=^H:bl=^G:cr=^M:it#8:ho=\\E[H:nw=\\EE:ta=^I:is=\\E)0:\\\n\t:li#24:co#80:am:xn:xv:LP:sr=\\EM:al=\\E[L:AL=\\E[%dL:\\\n\t:cs=\\E[%i%d;%dr:dl=\\E[M:DL=\\E[%dM:dc=\\E[P:DC=\\E[%dP:\\\n\t:im=\\E[4h:ei=\\E[4l:mi:IC=\\E[%d@:ks=\\E[?1h\\E=:\\\n\t:ke=\\E[?1l\\E>:vi=\\E[?25l:ve=\\E[34h\\E[?25h:vs=\\E[34l:\\\n\t:ti=\\E[?1049h:te=\\E[?1049l:Km=\\E[M:k0=\\E[10~:k1=\\EOP:\\\n\t:k2=\\EOQ:k3=\\EOR:k4=\\EOS:k5=\\E[15~:k6=\\E[17~:k7=\\E[18~:\\\n\t:k8=\\E[19~:k9=\\E[20~:k;=\\E[21~:F1=\\E[23~:F2=\\E[24~:\\\n\t:kh=\\E[1~:@1=\\E[1~:kH=\\E[4~:@7=\\E[4~:kN=\\E[6~:kP=\\E[5~:\\\n\t:kI=\\E[2~:kD=\\E[3~:ku=\\EOA:kd=\\EOB:kr=\\EOC:kl=\\EOD: |
int | timeout | -1 |
string | USER | imk1 |
string | UTIL_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/av_scripts/ |
int | walltimeout | 8640000 |
int | week | 604800 |
string | WINDOW | 0 |
string | XDG_RUNTIME_DIR | /run/user/1048 |
string | XDG_SESSION_ID | 20 |
string | XFILESEARCHPATH | /usr/dt/app-defaults/%L/Dt |