Script file | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/chipseq.bds |
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Program ID | chipseq.bds.20161109_175132_428 |
Start time | 2016-11-09 17:51:32 |
Run time | 03:33:47.272 |
Tasks executed | 3 |
Tasks failed | 2 |
Tasks failed names | bwa_aln rep1 markdup_bam_picard ctl1 |
Arguments* | [-title, SRR1370902_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370902_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
System* | sge |
Cpus* | -1 |
Exit value | 1 |
Thread ID | Parent | Tasks |
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thread_Root | None | |
thread_41 | thread_Root | chipseq.bds.20161109_175132_428_parallel_41/task.align_bwa.bwa_aln_rep1.line_108.id_10 |
thread_42 | thread_Root | chipseq.bds.20161109_175132_428_parallel_42/task.postalign_bam.dedup_bam_1_ctl1.line_95.id_11 chipseq.bds.20161109_175132_428_parallel_42/task.postalign_bam.markdup_bam_picard_ctl1.line_347.id_12 |
Task | Execution | Time | Dependencies | Task program, Errors, StdOut / StdErr | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 110 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 113 bwa aln -q 5 -l 32 -k 2 -t 1 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370902_1.fastq.gz > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/rep1/SRR1370902_1.sai # SYS command. line 115 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Error message-------------------- Task disappeared from cluster's queue. Task or node failure? --------------------Stdout-------------------- 11855 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- [bwa_aln] 17bp reads: max_diff = 2 [bwa_aln] 38bp reads: max_diff = 3 [bwa_aln] 64bp reads: max_diff = 4 [bwa_aln] 93bp reads: max_diff = 5 [bwa_aln] 124bp reads: max_diff = 6 [bwa_aln] 157bp reads: max_diff = 7 [bwa_aln] 190bp reads: max_diff = 8 [bwa_aln] 225bp reads: max_diff = 9 [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 74.55 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 262144 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 66.49 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 524288 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 62.69 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 786432 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 63.07 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1048576 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 64.68 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 1310720 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 63.31 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1572864 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 63.32 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 1835008 sequences have been processed. [bwa_read_seq] 1.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 63.23 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 2097152 sequences have been processed. [bwa_read_seq] 1.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 62.82 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 2359296 sequences have been processed. [bwa_read_seq] 1.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.77 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 2621440 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 87.86 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 2883584 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 95.26 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3145728 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.08 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3407872 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.34 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3670016 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 71.67 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 3932160 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 66.36 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 4194304 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 63.74 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 4456448 sequences have been processed. [bwa_read_seq] 1.0% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 79.22 sec [bwa_aln_core] write to the disk... 0.05 sec [bwa_aln_core] 4718592 sequences have been processed. [bwa_read_seq] 1.1% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 77.56 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 4980736 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 72.51 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 5242880 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.77 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 5505024 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 67.58 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 5767168 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 68.93 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 6029312 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 67.48 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 6291456 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.46 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 6553600 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 68.46 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 6815744 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.50 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 7077888 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.55 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 7340032 sequences have been processed. [bwa_read_seq] 0.7% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 70.60 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 7602176 sequences have been processed. [bwa_read_seq] 0.8% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 70.14 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 7864320 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.91 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 8126464 sequences have been processed. [bwa_read_seq] 0.9% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 71.17 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 8388608 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 69.04 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 8650752 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 68.11 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 8912896 sequences have been processed. [bwa_read_seq] 0.5% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 70.55 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 9175040 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 68.95 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 9437184 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 71.25 sec [bwa_aln_core] write to the disk... 0.03 sec [bwa_aln_core] 9699328 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 72.81 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 9961472 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 75.81 sec [bwa_aln_core] write to the disk... 0.04 sec [bwa_aln_core] 10223616 sequences have been processed. [bwa_read_seq] 0.6% bases are trimmed. [bwa_aln_core] calculate SA coordinate... 73.84 sec [bwa_aln_core] write to the disk... [fwrite] No space left on device | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 97 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 105 if [[ 0 > 0 ]]; then \ sambamba sort -t 4 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \ samtools view -F 1804 -Su /dev/stdin | \ sambamba sort -t 4 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.qnmsrt.bam; \ else \ samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam | \ sambamba sort -t 4 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam; \ fi # SYS command. line 116 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 29960 (process ID) old priority 0, new priority 10 | ||||||||||||||||||||||||||||||||||||||||
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| # SYS command. line 349 if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$ # SYS command. line 353 export _JAVA_OPTIONS="-Xms256M -Xmx4G -XX:ParallelGCThreads=1" # SYS command. line 354 export MAX_JAVA_MEM="12G" # SYS command. line 359 if [ -f "${PICARDROOT}/picard.jar" ]; then \ MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \ elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \ MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \ elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \ MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \ fi # SYS command. line 368 if [ -f "${MARKDUP}" ]; then \ java -Xmx4G -jar ${MARKDUP} \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ else \ picard MarkDuplicates \ INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam" \ METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc" VALIDATION_STRINGENCY=LENIENT \ ASSUME_SORTED=true REMOVE_DUPLICATES=false; \ fi # SYS command. line 380 TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi --------------------Stdout-------------------- 21019 (process ID) old priority 0, new priority 10 --------------------Stderr-------------------- Picked up _JAVA_OPTIONS: -Xms256M -Xmx4G -XX:ParallelGCThreads=1 [Wed Nov 09 21:22:01 PST 2016] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/align/ctl1/ss_50M_2000_GRCh38.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out/qc/ctl1/ss_50M_2000_GRCh38.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Wed Nov 09 21:22:01 PST 2016] Executing as imk1@surya on Linux 4.4.0-45-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater INFO 2016-11-09 21:22:01 MarkDuplicates Start of doWork freeMemory: 254879200; totalMemory: 257425408; maxMemory: 3817865216 INFO 2016-11-09 21:22:01 MarkDuplicates Reading input file and constructing read end information. INFO 2016-11-09 21:22:01 MarkDuplicates Will retain up to 14684096 data points before spilling to disk. INFO 2016-11-09 21:22:10 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:08s. Time for last 1,000,000: 8s. Last read position: chr1:22,298,551 INFO 2016-11-09 21:22:10 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-09 21:22:16 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:14s. Time for last 1,000,000: 6s. Last read position: chr1:41,895,424 INFO 2016-11-09 21:22:16 MarkDuplicates Tracking 8 as yet unmatched pairs. 8 records in RAM. INFO 2016-11-09 21:22:23 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:21s. Time for last 1,000,000: 6s. Last read position: chr1:63,396,527 INFO 2016-11-09 21:22:23 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-09 21:22:29 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:27s. Time for last 1,000,000: 5s. Last read position: chr1:89,147,707 INFO 2016-11-09 21:22:29 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-09 21:22:35 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:33s. Time for last 1,000,000: 6s. Last read position: chr1:115,615,553 INFO 2016-11-09 21:22:35 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-09 21:22:41 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:00:39s. Time for last 1,000,000: 5s. Last read position: chr1:164,468,682 INFO 2016-11-09 21:22:41 MarkDuplicates Tracking 6 as yet unmatched pairs. 6 records in RAM. INFO 2016-11-09 21:22:50 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:00:48s. Time for last 1,000,000: 8s. Last read position: chr1:189,102,252 INFO 2016-11-09 21:22:50 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-09 21:22:58 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:00:56s. Time for last 1,000,000: 7s. Last read position: chr1:213,979,760 INFO 2016-11-09 21:22:58 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. INFO 2016-11-09 21:23:04 MarkDuplicates Read 9,000,000 records. Elapsed time: 00:01:02s. Time for last 1,000,000: 6s. Last read position: chr1:231,814,268 INFO 2016-11-09 21:23:04 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-09 21:23:11 MarkDuplicates Read 10,000,000 records. Elapsed time: 00:01:09s. Time for last 1,000,000: 6s. Last read position: chr2:3,652,468 INFO 2016-11-09 21:23:11 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-09 21:23:19 MarkDuplicates Read 11,000,000 records. Elapsed time: 00:01:17s. Time for last 1,000,000: 8s. Last read position: chr2:39,505,122 INFO 2016-11-09 21:23:19 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-09 21:23:28 MarkDuplicates Read 12,000,000 records. Elapsed time: 00:01:26s. Time for last 1,000,000: 9s. Last read position: chr2:73,561,393 INFO 2016-11-09 21:23:28 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM. INFO 2016-11-09 21:23:35 MarkDuplicates Read 13,000,000 records. Elapsed time: 00:01:33s. Time for last 1,000,000: 6s. Last read position: chr2:120,526,571 INFO 2016-11-09 21:23:35 MarkDuplicates Tracking 2 as yet unmatched pairs. 2 records in RAM. INFO 2016-11-09 21:23:41 MarkDuplicates Read 14,000,000 records. Elapsed time: 00:01:39s. Time for last 1,000,000: 6s. Last read position: chr2:160,316,783 INFO 2016-11-09 21:23:41 MarkDuplicates Tracking 4 as yet unmatched pairs. 4 records in RAM. [Wed Nov 09 21:23:46 PST 2016] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 1.75 minutes. Runtime.totalMemory()=2586312704 To get help, see http://broadinstitute.github.io/picard/index.html#GettingHelp Exception in thread "main" htsjdk.samtools.util.RuntimeIOException: java.io.IOException: No space left on device at htsjdk.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:245) at htsjdk.samtools.util.SortingCollection.add(SortingCollection.java:165) at picard.sam.markduplicates.MarkDuplicates.buildSortedReadEndLists(MarkDuplicates.java:281) at picard.sam.markduplicates.MarkDuplicates.doWork(MarkDuplicates.java:114) at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:187) at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:89) at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:99) Caused by: java.io.IOException: No space left on device at java.io.FileOutputStream.writeBytes(Native Method) at java.io.FileOutputStream.write(FileOutputStream.java:326) at java.io.BufferedOutputStream.flushBuffer(BufferedOutputStream.java:82) at java.io.BufferedOutputStream.flush(BufferedOutputStream.java:140) at java.io.FilterOutputStream.close(FilterOutputStream.java:158) at htsjdk.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:236) ... 6 more --------------------Post mortem info-------------------- ============================================================== job_number: 6272 submission_time: Wed Nov 9 18:42:48 2016 owner: imk1 uid: 1048 group: users gid: 100 sge_o_home: /users/imk1/ sge_o_log_name: imk1 sge_o_path: /software/ascp/:/software/sratoolkit/2.5.2/bin/:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/software/miniconda3/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/users/imk1/edirect:/users/imk1/edirect sge_o_shell: /bin/bash sge_o_workdir: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1 sge_o_host: surya account: sge stderr_path_list: NONE:NONE:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/chipseq.bds.20161109_175132_428_parallel_42/task.postalign_bam.markdup_bam_picard_ctl1.line_347.id_12.stderr.cluster mail_list: imk1@surya notify: FALSE job_name: STDIN stdout_path_list: NONE:NONE:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/chipseq.bds.20161109_175132_428_parallel_42/task.postalign_bam.markdup_bam_picard_ctl1.line_347.id_12.stdout.cluster jobshare: 0 env_list: MAIL=/var/mail/imk1,SSH_CLIENT=171.65.77.8 55144 22,USER=imk1,J2SDKDIR=/usr/lib/jvm/java-8-oracle,SHLVL=3,PERL_LOCAL_LIB_ROOT=/users/imk1/perl5,J2REDIR=/usr/lib/jvm/java-8-oracle/jre,HOME=/users/imk1/,DEEPLIFT_DIR=/srv/scratch/imk1/TFBindingPredictionProject/src/deeplift_private/deeplift,SSH_TTY=/dev/pts/4,ASCPKEY=/software/ascp/etc/asperaweb_id_dsa.openssh,_LMFILES_=/software/modulefiles/modsappsdir/modsappsdir:/software/modulefiles/sratoolkit/2.5.2:/software/modulefiles/ascp/3.5.6,LOGNAME=imk1,_=/usr/bin/bds,XDG_SESSION_ID=20,TERM=screen,PERL_MB_OPT=--install_base "/users/imk1/perl5",KERAS_DIR=/users/imk1/.local/lib/python2.7/site-packages/keras/,SGE_ROOT=/var/lib/gridengine,PATH=/software/ascp/:/software/sratoolkit/2.5.2/bin/:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/software/miniconda3/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/users/imk1/edirect:/users/imk1/edirect,DERBY_HOME=/usr/lib/jvm/java-8-oracle/db,PERL5LIB=/users/imk1/perl5/lib/perl5,XDG_RUNTIME_DIR=/run/user/1048,DISPLAY=localhost:12.0,SGE_CELL=default,STY=14857.SRR1370902_1.BDS,LANG=en_US.UTF-8,SHELL=/bin/bash,KRB5CCNAME=FILE:/tmp/krb5cc_1048_N5SeQf,XFILESEARCHPATH=/usr/dt/app-defaults/%L/Dt,RULEFITBASE=/srv/scratch/imk1/TFBindingPredictionProject/src/RuleFit/,PERL_MM_OPT=INSTALL_BASE=/users/imk1/perl5,UTIL_SCRIPTS_DIR=/srv/scratch/imk1/TFBindingPredictionProject/src/av_scripts/,MODULE_VERSION=3.2.10,MODULE_VERSION_STACK=3.2.10,WINDOW=0,NLSPATH=/usr/dt/lib/nls/msg/%L/%N.cat,PWD=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1,JAVA_HOME=/usr/lib/jvm/java-8-oracle,LOADEDMODULES=modsappsdir/modsappsdir:sratoolkit/2.5.2:ascp/3.5.6,SSH_CONNECTION=171.65.77.8 55144 171.65.76.63 22,TERMCAP=SC|screen|VT 100/ANSI X3.64 virtual terminal:\,PYTHONPATH=/users/imk1/.local/lib/python2.7/site-packages/keras/:/srv/scratch/imk1/TFBindingPredictionProject/src/pybedtools/,ENHANCER_SCRIPTS_DIR=/srv/scratch/imk1/TFBindingPredictionProject/src/enhancer_prediction_code/,MODULEPATH=/usr/local/Modules/versions :/usr/local/Modules/$MODULE_VERSION/modulefiles :/modules/ :/software/modulefiles,MODULESAPPSDIR=/software,MODULESHOME=/software/env_module/3.2.10 parallel environment: shm range: 1 scheduling info: queue instance "amd.q@wotan" dropped because it is full queue instance "q@kadru" dropped because it is full queue instance "q@nandi" dropped because it is full queue instance "q@kali" dropped because it is full queue instance "q@surya" dropped because it is full |
Type | Name | Value |
---|---|---|
string | _ | /usr/bin/bds |
string | _LMFILES_ | /software/modulefiles/modsappsdir/modsappsdir:/software/modulefiles/sratoolkit/2.5.2:/software/modulefiles/ascp/3.5.6 |
bool | allowEmpty | false |
string[] | args | [-title, SRR1370902_1, -nth, 5, -macs2_for_pooled_rep_only, -species, hg38, -url_base, http://mitra.stanford.edu/kundaje/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1/out, -fastq, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/RawReadsFiles/SRR1370902_1.fastq.gz, -ctl_bam, /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/EncodeOtherZnfData/Encode3Datahg38/InputData/ss_50M_2000_GRCh38.bam, -final_stage, xcor, $] |
string | ASCPKEY | /software/ascp/etc/asperaweb_id_dsa.openssh |
bool | canFail | false |
int | cpus | -1 |
int | cpusLocal | 56 |
int | day | 86400 |
string | DEEPLIFT_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/deeplift_private/deeplift |
string | DERBY_HOME | /usr/lib/jvm/java-8-oracle/db |
string | DISPLAY | localhost:12.0 |
real | E | 2.718281828459045 |
string | ENHANCER_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/enhancer_prediction_code/ |
int | G | 1073741824 |
string | HOME | /users/imk1/ |
int | hour | 3600 |
string | J2REDIR | /usr/lib/jvm/java-8-oracle/jre |
string | J2SDKDIR | /usr/lib/jvm/java-8-oracle |
string | JAVA_HOME | /usr/lib/jvm/java-8-oracle |
int | K | 1024 |
string | KERAS_DIR | /users/imk1/.local/lib/python2.7/site-packages/keras/ |
string | KRB5CCNAME | FILE:/tmp/krb5cc_1048_N5SeQf |
string | LANG | en_US.UTF-8 |
string | LOADEDMODULES | modsappsdir/modsappsdir:sratoolkit/2.5.2:ascp/3.5.6 |
string | LOGNAME | imk1 |
int | M | 1048576 |
string | /var/mail/imk1 | |
int | mem | -1 |
int | minute | 60 |
string | MODULE_VERSION | 3.2.10 |
string | MODULE_VERSION_STACK | 3.2.10 |
string | MODULEPATH | /usr/local/Modules/versions\t\t\t\t:/usr/local/Modules/$MODULE_VERSION/modulefiles\t:/modules/\t\t\t\t:/software/modulefiles |
string | MODULESAPPSDIR | /software |
string | MODULESHOME | /software/env_module/3.2.10 |
string | NLSPATH | /usr/dt/lib/nls/msg/%L/%N.cat |
string | node | |
int | P | 1125899906842624 |
string | PATH | /software/ascp/:/software/sratoolkit/2.5.2/bin/:/users/imk1/anaconda2/bin:/users/imk1/perl5/bin:/srv/scratch/imk1/TFBindingPredictionProject/src/bedtools2/bin:/usr/local/cuda/bin:/srv/persistent/imk1/TFBindingPredictionProject/src/rcade/:/srv/scratch/imk1/TFBindingPredictionProject/src/R-3.2.3/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/software/miniconda3/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/users/imk1/edirect:/users/imk1/edirect |
string | PERL5LIB | /users/imk1/perl5/lib/perl5 |
string | PERL_LOCAL_LIB_ROOT | /users/imk1/perl5 |
string | PERL_MB_OPT | --install_base \"/users/imk1/perl5\" |
string | PERL_MM_OPT | INSTALL_BASE=/users/imk1/perl5 |
real | PI | 3.141592653589793 |
string | ppwd | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1 |
string | programName | chipseq.bds |
string | programPath | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/chipseq.bds |
string | PWD | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/HughesZnfData/SRR1370902_1 |
string | PYTHONPATH | /users/imk1/.local/lib/python2.7/site-packages/keras/:/srv/scratch/imk1/TFBindingPredictionProject/src/pybedtools/ |
string | queue | |
int | retry | 0 |
string | RULEFITBASE | /srv/scratch/imk1/TFBindingPredictionProject/src/RuleFit/ |
string | SHELL | /bin/bash |
string | SHLVL | 3 |
string | SSH_CLIENT | 171.65.77.8 55144 22 |
string | SSH_CONNECTION | 171.65.77.8 55144 171.65.76.63 22 |
string | SSH_TTY | /dev/pts/4 |
string | STY | 14857.SRR1370902_1.BDS |
string | system | sge |
int | T | 1099511627776 |
string | TERM | screen |
string | TERMCAP | SC|screen|VT 100/ANSI X3.64 virtual terminal:\\\n\t:DO=\\E[%dB:LE=\\E[%dD:RI=\\E[%dC:UP=\\E[%dA:bs:bt=\\E[Z:\\\n\t:cd=\\E[J:ce=\\E[K:cl=\\E[H\\E[J:cm=\\E[%i%d;%dH:ct=\\E[3g:\\\n\t:do=^J:nd=\\E[C:pt:rc=\\E8:rs=\\Ec:sc=\\E7:st=\\EH:up=\\EM:\\\n\t:le=^H:bl=^G:cr=^M:it#8:ho=\\E[H:nw=\\EE:ta=^I:is=\\E)0:\\\n\t:li#24:co#80:am:xn:xv:LP:sr=\\EM:al=\\E[L:AL=\\E[%dL:\\\n\t:cs=\\E[%i%d;%dr:dl=\\E[M:DL=\\E[%dM:dc=\\E[P:DC=\\E[%dP:\\\n\t:im=\\E[4h:ei=\\E[4l:mi:IC=\\E[%d@:ks=\\E[?1h\\E=:\\\n\t:ke=\\E[?1l\\E>:vi=\\E[?25l:ve=\\E[34h\\E[?25h:vs=\\E[34l:\\\n\t:ti=\\E[?1049h:te=\\E[?1049l:Km=\\E[M:k0=\\E[10~:k1=\\EOP:\\\n\t:k2=\\EOQ:k3=\\EOR:k4=\\EOS:k5=\\E[15~:k6=\\E[17~:k7=\\E[18~:\\\n\t:k8=\\E[19~:k9=\\E[20~:k;=\\E[21~:F1=\\E[23~:F2=\\E[24~:\\\n\t:kh=\\E[1~:@1=\\E[1~:kH=\\E[4~:@7=\\E[4~:kN=\\E[6~:kP=\\E[5~:\\\n\t:kI=\\E[2~:kD=\\E[3~:ku=\\EOA:kd=\\EOB:kr=\\EOC:kl=\\EOD: |
int | timeout | -1 |
string | USER | imk1 |
string | UTIL_SCRIPTS_DIR | /srv/scratch/imk1/TFBindingPredictionProject/src/av_scripts/ |
int | walltimeout | 8640000 |
int | week | 604800 |
string | WINDOW | 0 |
string | XDG_RUNTIME_DIR | /run/user/1048 |
string | XDG_SESSION_ID | 20 |
string | XFILESEARCHPATH | /usr/dt/app-defaults/%L/Dt |