Num | 1 | ID | task.callpeak_macs2.macs2_rep1.line_67.id_10 | Name | macs2 rep1 | Thread | thread_Root | PID | 4235 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2016-11-06 18:08:15 | End | 2016-11-06 18:25:53 | Elapsed | 00:17:38 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/signal/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bw
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/signal/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bw
| Dependencies | | |
# SYS command. line 69
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 70
export LC_COLLATE=C
# SYS command. line 75
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# SYS command. line 78
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
# SYS command. line 81
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 88
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# SYS command. line 91
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.broadPeak.gz
# SYS command. line 92
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
# SYS command. line 95
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 100
if [[ true == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 111
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1 -o "SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 114
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 115
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg
# SYS command. line 118
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 119
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/signal/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bw
# SYS command. line 120
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 126
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 128
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 130
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1 -o "SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 133
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 134
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg
# SYS command. line 137
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 138
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/signal/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bw
# SYS command. line 139
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 141
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 143
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
33065 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
discarding /software/miniconda3/bin from PATH
prepending /software/miniconda3/envs/aquas_chipseq/bin to PATH
INFO @ Sun, 06 Nov 2016 18:08:29:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Sun, 06 Nov 2016 18:08:29: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:08:29: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:08:31: 1000000
INFO @ Sun, 06 Nov 2016 18:08:33: 2000000
INFO @ Sun, 06 Nov 2016 18:08:35: 3000000
INFO @ Sun, 06 Nov 2016 18:08:36: 4000000
INFO @ Sun, 06 Nov 2016 18:08:38: 5000000
INFO @ Sun, 06 Nov 2016 18:08:39: 6000000
INFO @ Sun, 06 Nov 2016 18:08:41: 7000000
INFO @ Sun, 06 Nov 2016 18:08:42: 8000000
INFO @ Sun, 06 Nov 2016 18:08:44: 9000000
INFO @ Sun, 06 Nov 2016 18:08:45: 10000000
INFO @ Sun, 06 Nov 2016 18:08:47: 11000000
INFO @ Sun, 06 Nov 2016 18:08:48: 12000000
INFO @ Sun, 06 Nov 2016 18:08:50: 13000000
INFO @ Sun, 06 Nov 2016 18:08:51: 14000000
INFO @ Sun, 06 Nov 2016 18:08:53: 15000000
INFO @ Sun, 06 Nov 2016 18:08:55: 16000000
INFO @ Sun, 06 Nov 2016 18:08:56: 17000000
INFO @ Sun, 06 Nov 2016 18:08:58: 18000000
INFO @ Sun, 06 Nov 2016 18:08:59: 19000000
INFO @ Sun, 06 Nov 2016 18:09:01: 20000000
INFO @ Sun, 06 Nov 2016 18:09:02: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:09:03: 1000000
INFO @ Sun, 06 Nov 2016 18:09:05: 2000000
INFO @ Sun, 06 Nov 2016 18:09:06: 3000000
INFO @ Sun, 06 Nov 2016 18:09:07: #1 tag size is determined as 50 bps
INFO @ Sun, 06 Nov 2016 18:09:07: #1 tag size = 50
INFO @ Sun, 06 Nov 2016 18:09:07: #1 total tags in treatment: 20609670
INFO @ Sun, 06 Nov 2016 18:09:07: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:09:07: #1 finished!
INFO @ Sun, 06 Nov 2016 18:09:07: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:09:07: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:09:07: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:09:07: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:09:07: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Sun, 06 Nov 2016 18:09:07: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:09:40: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Sun, 06 Nov 2016 18:09:40: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_treat_pileup.bdg
INFO @ Sun, 06 Nov 2016 18:09:40: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_control_lambda.bdg
INFO @ Sun, 06 Nov 2016 18:09:40: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Sun, 06 Nov 2016 18:09:40: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:11:36: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:11:36: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.narrowPeak
INFO @ Sun, 06 Nov 2016 18:11:36: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_summits.bed
INFO @ Sun, 06 Nov 2016 18:11:36: Done!
INFO @ Sun, 06 Nov 2016 18:11:38:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/rep1/SRR836479.nodup.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Sun, 06 Nov 2016 18:11:38: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:11:38: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:11:40: 1000000
INFO @ Sun, 06 Nov 2016 18:11:41: 2000000
INFO @ Sun, 06 Nov 2016 18:11:43: 3000000
INFO @ Sun, 06 Nov 2016 18:11:44: 4000000
INFO @ Sun, 06 Nov 2016 18:11:46: 5000000
INFO @ Sun, 06 Nov 2016 18:11:47: 6000000
INFO @ Sun, 06 Nov 2016 18:11:49: 7000000
INFO @ Sun, 06 Nov 2016 18:11:50: 8000000
INFO @ Sun, 06 Nov 2016 18:11:51: 9000000
INFO @ Sun, 06 Nov 2016 18:11:53: 10000000
INFO @ Sun, 06 Nov 2016 18:11:54: 11000000
INFO @ Sun, 06 Nov 2016 18:11:56: 12000000
INFO @ Sun, 06 Nov 2016 18:11:57: 13000000
INFO @ Sun, 06 Nov 2016 18:11:59: 14000000
INFO @ Sun, 06 Nov 2016 18:12:00: 15000000
INFO @ Sun, 06 Nov 2016 18:12:02: 16000000
INFO @ Sun, 06 Nov 2016 18:12:03: 17000000
INFO @ Sun, 06 Nov 2016 18:12:05: 18000000
INFO @ Sun, 06 Nov 2016 18:12:07: 19000000
INFO @ Sun, 06 Nov 2016 18:12:08: 20000000
INFO @ Sun, 06 Nov 2016 18:12:09: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:12:11: 1000000
INFO @ Sun, 06 Nov 2016 18:12:12: 2000000
INFO @ Sun, 06 Nov 2016 18:12:14: 3000000
INFO @ Sun, 06 Nov 2016 18:12:14: #1 tag size is determined as 50 bps
INFO @ Sun, 06 Nov 2016 18:12:14: #1 tag size = 50
INFO @ Sun, 06 Nov 2016 18:12:14: #1 total tags in treatment: 20609670
INFO @ Sun, 06 Nov 2016 18:12:14: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:12:14: #1 finished!
INFO @ Sun, 06 Nov 2016 18:12:14: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:12:14: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:12:14: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:12:14: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:12:14: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Sun, 06 Nov 2016 18:12:14: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:12:57: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:13:13: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:13:13: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.broadPeak
INFO @ Sun, 06 Nov 2016 18:13:13: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.gappedPeak
INFO @ Sun, 06 Nov 2016 18:13:13: Done!
INFO @ Sun, 06 Nov 2016 18:13:16: Read and build treatment bedGraph...
INFO @ Sun, 06 Nov 2016 18:13:55: Read and build control bedGraph...
INFO @ Sun, 06 Nov 2016 18:14:06: Build scoreTrackII...
INFO @ Sun, 06 Nov 2016 18:14:21: Calculate scores comparing treatment and control by 'FE'...
INFO @ Sun, 06 Nov 2016 18:16:20: Write bedGraph of scores...
INFO @ Sun, 06 Nov 2016 18:17:59: Finished 'FE'! Please check '/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_FE.bdg'!
INFO @ Sun, 06 Nov 2016 18:21:22: Read and build treatment bedGraph...
INFO @ Sun, 06 Nov 2016 18:22:00: Read and build control bedGraph...
INFO @ Sun, 06 Nov 2016 18:22:11: Build scoreTrackII...
INFO @ Sun, 06 Nov 2016 18:22:25: Values in your input bedGraph files will be multiplied by 3.384182 ...
INFO @ Sun, 06 Nov 2016 18:24:24: Calculate scores comparing treatment and control by 'ppois'...
INFO @ Sun, 06 Nov 2016 18:24:36: Write bedGraph of scores...
INFO @ Sun, 06 Nov 2016 18:25:03: Finished 'ppois'! Please check '/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/rep1/SRR836479.nodup.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_ppois.bdg'!
|
Num | 2 | ID | task.callpeak_macs2.macs2_rep1_pr1.line_67.id_11 | Name | macs2 rep1-pr1 | Thread | thread_Root | PID | 4246 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2016-11-06 18:08:19 | End | 2016-11-06 18:11:42 | Elapsed | 00:03:22 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
| Dependencies | | |
# SYS command. line 69
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 70
export LC_COLLATE=C
# SYS command. line 75
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# SYS command. line 78
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
# SYS command. line 81
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 88
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# SYS command. line 91
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.broadPeak.gz
# SYS command. line 92
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
# SYS command. line 95
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 100
if [[ false == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 111
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1 -o "SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 114
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 115
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg
# SYS command. line 118
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 119
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bw
# SYS command. line 120
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 126
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 128
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 130
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1 -o "SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 133
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 134
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg
# SYS command. line 137
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 138
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bw
# SYS command. line 139
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 141
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 143
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
44681 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
discarding /software/miniconda3/bin from PATH
prepending /software/miniconda3/envs/aquas_chipseq/bin to PATH
INFO @ Sun, 06 Nov 2016 18:08:30:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Sun, 06 Nov 2016 18:08:30: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:08:30: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:08:33: 1000000
INFO @ Sun, 06 Nov 2016 18:08:35: 2000000
INFO @ Sun, 06 Nov 2016 18:08:36: 3000000
INFO @ Sun, 06 Nov 2016 18:08:38: 4000000
INFO @ Sun, 06 Nov 2016 18:08:39: 5000000
INFO @ Sun, 06 Nov 2016 18:08:41: 6000000
INFO @ Sun, 06 Nov 2016 18:08:42: 7000000
INFO @ Sun, 06 Nov 2016 18:08:44: 8000000
INFO @ Sun, 06 Nov 2016 18:08:46: 9000000
INFO @ Sun, 06 Nov 2016 18:08:47: 10000000
INFO @ Sun, 06 Nov 2016 18:08:48: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:08:50: 1000000
INFO @ Sun, 06 Nov 2016 18:08:52: 2000000
INFO @ Sun, 06 Nov 2016 18:08:53: 3000000
INFO @ Sun, 06 Nov 2016 18:08:54: #1 tag size is determined as 49 bps
INFO @ Sun, 06 Nov 2016 18:08:54: #1 tag size = 49
INFO @ Sun, 06 Nov 2016 18:08:54: #1 total tags in treatment: 10304835
INFO @ Sun, 06 Nov 2016 18:08:54: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:08:54: #1 finished!
INFO @ Sun, 06 Nov 2016 18:08:54: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:08:54: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:08:54: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:08:54: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:08:54: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Sun, 06 Nov 2016 18:08:54: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:09:13: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Sun, 06 Nov 2016 18:09:13: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_treat_pileup.bdg
INFO @ Sun, 06 Nov 2016 18:09:13: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_control_lambda.bdg
INFO @ Sun, 06 Nov 2016 18:09:13: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Sun, 06 Nov 2016 18:09:13: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:10:34: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:10:35: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.narrowPeak
INFO @ Sun, 06 Nov 2016 18:10:38: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_summits.bed
INFO @ Sun, 06 Nov 2016 18:10:39: Done!
INFO @ Sun, 06 Nov 2016 18:10:42:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Sun, 06 Nov 2016 18:10:42: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:10:42: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:10:44: 1000000
INFO @ Sun, 06 Nov 2016 18:10:45: 2000000
INFO @ Sun, 06 Nov 2016 18:10:47: 3000000
INFO @ Sun, 06 Nov 2016 18:10:49: 4000000
INFO @ Sun, 06 Nov 2016 18:10:50: 5000000
INFO @ Sun, 06 Nov 2016 18:10:52: 6000000
INFO @ Sun, 06 Nov 2016 18:10:53: 7000000
INFO @ Sun, 06 Nov 2016 18:10:55: 8000000
INFO @ Sun, 06 Nov 2016 18:10:57: 9000000
INFO @ Sun, 06 Nov 2016 18:10:58: 10000000
INFO @ Sun, 06 Nov 2016 18:10:59: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:11:01: 1000000
INFO @ Sun, 06 Nov 2016 18:11:03: 2000000
INFO @ Sun, 06 Nov 2016 18:11:04: 3000000
INFO @ Sun, 06 Nov 2016 18:11:05: #1 tag size is determined as 49 bps
INFO @ Sun, 06 Nov 2016 18:11:05: #1 tag size = 49
INFO @ Sun, 06 Nov 2016 18:11:05: #1 total tags in treatment: 10304835
INFO @ Sun, 06 Nov 2016 18:11:05: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:11:05: #1 finished!
INFO @ Sun, 06 Nov 2016 18:11:05: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:11:05: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:11:05: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:11:05: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:11:05: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Sun, 06 Nov 2016 18:11:05: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:11:24: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:11:34: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:11:37: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.broadPeak
INFO @ Sun, 06 Nov 2016 18:11:39: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr1/SRR836479.nodup.pr1.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.gappedPeak
INFO @ Sun, 06 Nov 2016 18:11:39: Done!
|
Num | 3 | ID | task.callpeak_macs2.macs2_rep1_pr2.line_67.id_12 | Name | macs2 rep1-pr2 | Thread | thread_Root | PID | 4252 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2016-11-06 18:08:20 | End | 2016-11-06 18:12:50 | Elapsed | 00:04:29 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
| Dependencies | | |
# SYS command. line 69
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/TF_chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 70
export LC_COLLATE=C
# SYS command. line 75
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# SYS command. line 78
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.narrowPeak.gz
# SYS command. line 81
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 88
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# SYS command. line 91
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.broadPeak.gz
# SYS command. line 92
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -c > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gappedPeak.gz
# SYS command. line 95
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_summits.bed
# SYS command. line 100
if [[ false == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 111
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2 -o "SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 114
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 115
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_FE.bdg
# SYS command. line 118
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 119
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bw
# SYS command. line 120
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 126
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 128
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 130
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2 -o "SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 133
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 134
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_ppois.bdg
# SYS command. line 137
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 138
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/mm10/mm10.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bw
# SYS command. line 139
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 141
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 143
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
43149 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
discarding /software/miniconda3/bin from PATH
prepending /software/miniconda3/envs/aquas_chipseq/bin to PATH
INFO @ Sun, 06 Nov 2016 18:08:32:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --nomodel --shift 0 --extsize 125 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Sun, 06 Nov 2016 18:08:32: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:08:32: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:08:35: 1000000
INFO @ Sun, 06 Nov 2016 18:08:37: 2000000
INFO @ Sun, 06 Nov 2016 18:08:39: 3000000
INFO @ Sun, 06 Nov 2016 18:08:42: 4000000
INFO @ Sun, 06 Nov 2016 18:08:45: 5000000
INFO @ Sun, 06 Nov 2016 18:08:47: 6000000
INFO @ Sun, 06 Nov 2016 18:08:49: 7000000
INFO @ Sun, 06 Nov 2016 18:08:52: 8000000
INFO @ Sun, 06 Nov 2016 18:08:54: 9000000
INFO @ Sun, 06 Nov 2016 18:08:56: 10000000
INFO @ Sun, 06 Nov 2016 18:08:57: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:09:00: 1000000
INFO @ Sun, 06 Nov 2016 18:09:02: 2000000
INFO @ Sun, 06 Nov 2016 18:09:04: 3000000
INFO @ Sun, 06 Nov 2016 18:09:05: #1 tag size is determined as 49 bps
INFO @ Sun, 06 Nov 2016 18:09:05: #1 tag size = 49
INFO @ Sun, 06 Nov 2016 18:09:05: #1 total tags in treatment: 10304835
INFO @ Sun, 06 Nov 2016 18:09:05: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:09:05: #1 finished!
INFO @ Sun, 06 Nov 2016 18:09:05: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:09:05: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:09:05: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:09:05: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:09:05: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Sun, 06 Nov 2016 18:09:05: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:09:35: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Sun, 06 Nov 2016 18:09:35: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_treat_pileup.bdg
INFO @ Sun, 06 Nov 2016 18:09:35: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_control_lambda.bdg
INFO @ Sun, 06 Nov 2016 18:09:35: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Sun, 06 Nov 2016 18:09:35: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:11:09: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:11:10: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.narrowPeak
INFO @ Sun, 06 Nov 2016 18:11:10: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_summits.bed
INFO @ Sun, 06 Nov 2016 18:11:10: Done!
INFO @ Sun, 06 Nov 2016 18:11:14:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign -g mm -p 0.01 --broad --nomodel --shift 0 --extsize 125 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/align/ctl1/r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign.gz']
# effective genome size = 1.87e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Sun, 06 Nov 2016 18:11:14: #1 read tag files...
INFO @ Sun, 06 Nov 2016 18:11:14: #1 read treatment tags...
INFO @ Sun, 06 Nov 2016 18:11:16: 1000000
INFO @ Sun, 06 Nov 2016 18:11:18: 2000000
INFO @ Sun, 06 Nov 2016 18:11:20: 3000000
INFO @ Sun, 06 Nov 2016 18:11:22: 4000000
INFO @ Sun, 06 Nov 2016 18:11:24: 5000000
INFO @ Sun, 06 Nov 2016 18:11:27: 6000000
INFO @ Sun, 06 Nov 2016 18:11:29: 7000000
INFO @ Sun, 06 Nov 2016 18:11:31: 8000000
INFO @ Sun, 06 Nov 2016 18:11:33: 9000000
INFO @ Sun, 06 Nov 2016 18:11:35: 10000000
INFO @ Sun, 06 Nov 2016 18:11:37: #1.2 read input tags...
INFO @ Sun, 06 Nov 2016 18:11:40: 1000000
INFO @ Sun, 06 Nov 2016 18:11:43: 2000000
INFO @ Sun, 06 Nov 2016 18:11:44: 3000000
INFO @ Sun, 06 Nov 2016 18:11:45: #1 tag size is determined as 49 bps
INFO @ Sun, 06 Nov 2016 18:11:45: #1 tag size = 49
INFO @ Sun, 06 Nov 2016 18:11:45: #1 total tags in treatment: 10304835
INFO @ Sun, 06 Nov 2016 18:11:45: #1 total tags in control: 3384182
INFO @ Sun, 06 Nov 2016 18:11:45: #1 finished!
INFO @ Sun, 06 Nov 2016 18:11:45: #2 Build Peak Model...
INFO @ Sun, 06 Nov 2016 18:11:45: #2 Skipped...
INFO @ Sun, 06 Nov 2016 18:11:45: #2 Use 125 as fragment length
INFO @ Sun, 06 Nov 2016 18:11:45: #3 Call peaks...
INFO @ Sun, 06 Nov 2016 18:11:45: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Sun, 06 Nov 2016 18:11:45: #3 Pre-compute pvalue-qvalue table...
INFO @ Sun, 06 Nov 2016 18:12:19: #3 Call peaks for each chromosome...
INFO @ Sun, 06 Nov 2016 18:12:34: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.xls
INFO @ Sun, 06 Nov 2016 18:12:39: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.broadPeak
INFO @ Sun, 06 Nov 2016 18:12:43: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/MouseMutantData/SRR836479/out/peak/macs2/pseudo_reps/rep1/pr2/SRR836479.nodup.pr2.tagAlign_x_r120621A_l1i7_aB180__wt__bioBirAonly.nodup.tagAlign_peaks.gappedPeak
INFO @ Sun, 06 Nov 2016 18:12:44: Done!
|