Task | Execution | Time | Dependencies | Task program, Errors, StdOut / StdErr |
Num | 1 | ID | task.align_bwa.bwa_aln_ctl1.line_111.id_10 | Name | bwa_aln ctl1 | Thread | thread_42 | PID | 35697 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 15:56:47 | End | 2017-01-27 16:15:36 | Elapsed | 00:18:48 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.sai
| Dependencies | | |
# SYS command. line 113
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 116
bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.sai
# SYS command. line 118
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
40589 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
[bwa_aln] 17bp reads: max_diff = 2
[bwa_aln] 38bp reads: max_diff = 3
[bwa_aln] 64bp reads: max_diff = 4
[bwa_aln] 93bp reads: max_diff = 5
[bwa_aln] 124bp reads: max_diff = 6
[bwa_aln] 157bp reads: max_diff = 7
[bwa_aln] 190bp reads: max_diff = 8
[bwa_aln] 225bp reads: max_diff = 9
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.07 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 262144 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 60.47 sec
[bwa_aln_core] write to the disk... 0.07 sec
[bwa_aln_core] 524288 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 60.04 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 786432 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.69 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 1048576 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 59.57 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 1310720 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 59.99 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 1572864 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 55.76 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 1835008 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 56.75 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 2097152 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.93 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 2359296 sequences have been processed.
[bwa_read_seq] 3.6% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.24 sec
[bwa_aln_core] write to the disk... 0.07 sec
[bwa_aln_core] 2621440 sequences have been processed.
[bwa_read_seq] 3.5% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 55.03 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 2883584 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 57.55 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 3145728 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 55.82 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 3407872 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.93 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 3670016 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.61 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 3932160 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 56.74 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 4194304 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 55.95 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 4456448 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.53 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 4718592 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 57.25 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 4980736 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 57.49 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 5242880 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 61.97 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 5505024 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 62.08 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 5767168 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 63.74 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 6029312 sequences have been processed.
[bwa_read_seq] 3.6% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.78 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 6291456 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.14 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 6553600 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.45 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 6815744 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 56.06 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 7077888 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 57.38 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 7340032 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 56.39 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 7602176 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 57.72 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 7864320 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.15 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 8126464 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 54.93 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 8388608 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 51.79 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 8650752 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 52.99 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 8912896 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 58.17 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 9175040 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 56.04 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 9437184 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 53.87 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 9699328 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 19.30 sec
[bwa_aln_core] write to the disk... 0.02 sec
[bwa_aln_core] 9795918 sequences have been processed.
[main] Version: 0.7.13-r1126
[main] CMD: bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz
[main] Real time: 1102.980 sec; CPU: 2157.504 sec
|
Num | 2 | ID | task.align_bwa.bwa_aln_rep1.line_111.id_11 | Name | bwa_aln rep1 | Thread | thread_41 | PID | 35698 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 15:56:47 | End | 2017-01-27 16:12:03 | Elapsed | 00:15:16 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.sai
| Dependencies | | |
# SYS command. line 113
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 116
bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.sai
# SYS command. line 118
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
40590 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
[bwa_aln] 17bp reads: max_diff = 2
[bwa_aln] 38bp reads: max_diff = 3
[bwa_aln] 64bp reads: max_diff = 4
[bwa_aln] 93bp reads: max_diff = 5
[bwa_aln] 124bp reads: max_diff = 6
[bwa_aln] 157bp reads: max_diff = 7
[bwa_aln] 190bp reads: max_diff = 8
[bwa_aln] 225bp reads: max_diff = 9
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.24 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 262144 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 47.27 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 524288 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.17 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 786432 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.65 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 1048576 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.49 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 1310720 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.69 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 1572864 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.56 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 1835008 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.50 sec
[bwa_aln_core] write to the disk... 0.08 sec
[bwa_aln_core] 2097152 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.29 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 2359296 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.37 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 2621440 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.65 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 2883584 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.14 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 3145728 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.48 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 3407872 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 47.80 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 3670016 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 46.85 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 3932160 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.43 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 4194304 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.37 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 4456448 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 43.20 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 4718592 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.00 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 4980736 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.06 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 5242880 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 42.72 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 5505024 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.69 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 5767168 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 49.22 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 6029312 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.34 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 6291456 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 47.93 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 6553600 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 49.80 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 6815744 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 47.65 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 7077888 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 49.99 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 7340032 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 48.98 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 7602176 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 47.07 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 7864320 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 43.45 sec
[bwa_aln_core] write to the disk... 0.06 sec
[bwa_aln_core] 8126464 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 42.08 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 8388608 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 41.98 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 8650752 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.01 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 8912896 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.25 sec
[bwa_aln_core] write to the disk... 0.04 sec
[bwa_aln_core] 9175040 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 45.22 sec
[bwa_aln_core] write to the disk... 0.07 sec
[bwa_aln_core] 9437184 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 44.86 sec
[bwa_aln_core] write to the disk... 0.05 sec
[bwa_aln_core] 9699328 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] calculate SA coordinate... 18.66 sec
[bwa_aln_core] write to the disk... 0.02 sec
[bwa_aln_core] 9795918 sequences have been processed.
[main] Version: 0.7.13-r1126
[main] CMD: bwa aln -q 5 -l 32 -k 2 -t 2 /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz
[main] Real time: 890.211 sec; CPU: 1733.796 sec
|
Num | 3 | ID | task.align_bwa.bwa_sam_rep1.line_143.id_12 | Name | bwa_sam rep1 | Thread | thread_41 | PID | 35699 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:12:05 | End | 2017-01-27 16:45:14 | Elapsed | 00:33:09 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.sai
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.flagstat.qc
| Dependencies | | |
# SYS command. line 145
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 150
bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz | samtools view -Su /dev/stdin \
| sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam
# SYS command. line 152
sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.flagstat.qc
# SYS command. line 154
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
26535 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 37.82 sec
[bwa_aln_core] refine gapped alignments... 6.59 sec
[bwa_aln_core] print alignments... [samopen] SAM header is present: 493 sequences.
0.34 sec
[bwa_aln_core] 262144 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.50 sec
[bwa_aln_core] refine gapped alignments... 5.11 sec
[bwa_aln_core] print alignments... 0.35 sec
[bwa_aln_core] 524288 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.30 sec
[bwa_aln_core] refine gapped alignments... 5.15 sec
[bwa_aln_core] print alignments... 0.34 sec
[bwa_aln_core] 786432 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.29 sec
[bwa_aln_core] refine gapped alignments... 5.11 sec
[bwa_aln_core] print alignments... 0.34 sec
[bwa_aln_core] 1048576 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 33.11 sec
[bwa_aln_core] refine gapped alignments... 5.21 sec
[bwa_aln_core] print alignments... 0.72 sec
[bwa_aln_core] 1310720 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 40.28 sec
[bwa_aln_core] refine gapped alignments... 4.45 sec
[bwa_aln_core] print alignments... 0.33 sec
[bwa_aln_core] 1572864 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.44 sec
[bwa_aln_core] refine gapped alignments... 4.89 sec
[bwa_aln_core] print alignments... 0.47 sec
[bwa_aln_core] 1835008 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 41.26 sec
[bwa_aln_core] refine gapped alignments... 5.30 sec
[bwa_aln_core] print alignments... 0.43 sec
[bwa_aln_core] 2097152 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 41.41 sec
[bwa_aln_core] refine gapped alignments... 5.53 sec
[bwa_aln_core] print alignments... 0.42 sec
[bwa_aln_core] 2359296 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.07 sec
[bwa_aln_core] refine gapped alignments... 6.14 sec
[bwa_aln_core] print alignments... 0.34 sec
[bwa_aln_core] 2621440 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.35 sec
[bwa_aln_core] refine gapped alignments... 6.36 sec
[bwa_aln_core] print alignments... 0.33 sec
[bwa_aln_core] 2883584 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.68 sec
[bwa_aln_core] refine gapped alignments... 6.94 sec
[bwa_aln_core] print alignments... 0.57 sec
[bwa_aln_core] 3145728 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.03 sec
[bwa_aln_core] refine gapped alignments... 6.96 sec
[bwa_aln_core] print alignments... 0.69 sec
[bwa_aln_core] 3407872 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.69 sec
[bwa_aln_core] refine gapped alignments... 6.98 sec
[bwa_aln_core] print alignments... 0.37 sec
[bwa_aln_core] 3670016 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.29 sec
[bwa_aln_core] refine gapped alignments... 7.06 sec
[bwa_aln_core] print alignments... 0.48 sec
[bwa_aln_core] 3932160 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.93 sec
[bwa_aln_core] refine gapped alignments... 6.92 sec
[bwa_aln_core] print alignments... 0.61 sec
[bwa_aln_core] 4194304 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.69 sec
[bwa_aln_core] refine gapped alignments... 6.91 sec
[bwa_aln_core] print alignments... 0.60 sec
[bwa_aln_core] 4456448 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 40.47 sec
[bwa_aln_core] refine gapped alignments... 5.79 sec
[bwa_aln_core] print alignments... 0.49 sec
[bwa_aln_core] 4718592 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.43 sec
[bwa_aln_core] refine gapped alignments... 4.06 sec
[bwa_aln_core] print alignments... 0.44 sec
[bwa_aln_core] 4980736 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 32.13 sec
[bwa_aln_core] refine gapped alignments... 5.02 sec
[bwa_aln_core] print alignments... 0.39 sec
[bwa_aln_core] 5242880 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 25.59 sec
[bwa_aln_core] refine gapped alignments... 4.69 sec
[bwa_aln_core] print alignments... 0.62 sec
[bwa_aln_core] 5505024 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 30.09 sec
[bwa_aln_core] refine gapped alignments... 5.38 sec
[bwa_aln_core] print alignments... 0.47 sec
[bwa_aln_core] 5767168 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 27.01 sec
[bwa_aln_core] refine gapped alignments... 5.63 sec
[bwa_aln_core] print alignments... 0.55 sec
[bwa_aln_core] 6029312 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.30 sec
[bwa_aln_core] refine gapped alignments... 4.59 sec
[bwa_aln_core] print alignments... 0.59 sec
[bwa_aln_core] 6291456 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 28.67 sec
[bwa_aln_core] refine gapped alignments... 5.13 sec
[bwa_aln_core] print alignments... 0.47 sec
[bwa_aln_core] 6553600 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 71.94 sec
[bwa_aln_core] refine gapped alignments... 11.55 sec
[bwa_aln_core] print alignments... 0.56 sec
[bwa_aln_core] 6815744 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 35.74 sec
[bwa_aln_core] refine gapped alignments... 6.58 sec
[bwa_aln_core] print alignments... 0.65 sec
[bwa_aln_core] 7077888 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.99 sec
[bwa_aln_core] refine gapped alignments... 6.32 sec
[bwa_aln_core] print alignments... 0.72 sec
[bwa_aln_core] 7340032 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 44.49 sec
[bwa_aln_core] refine gapped alignments... 6.84 sec
[bwa_aln_core] print alignments... 0.71 sec
[bwa_aln_core] 7602176 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 45.35 sec
[bwa_aln_core] refine gapped alignments... 8.64 sec
[bwa_aln_core] print alignments... 0.63 sec
[bwa_aln_core] 7864320 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 72.19 sec
[bwa_aln_core] refine gapped alignments... 17.88 sec
[bwa_aln_core] print alignments... 0.66 sec
[bwa_aln_core] 8126464 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 124.78 sec
[bwa_aln_core] refine gapped alignments... 11.99 sec
[bwa_aln_core] print alignments... 0.40 sec
[bwa_aln_core] 8388608 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 40.63 sec
[bwa_aln_core] refine gapped alignments... 6.08 sec
[bwa_aln_core] print alignments... 0.39 sec
[bwa_aln_core] 8650752 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 37.40 sec
[bwa_aln_core] refine gapped alignments... 4.65 sec
[bwa_aln_core] print alignments... 0.39 sec
[bwa_aln_core] 8912896 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 33.27 sec
[bwa_aln_core] refine gapped alignments... 6.91 sec
[bwa_aln_core] print alignments... 0.41 sec
[bwa_aln_core] 9175040 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 32.77 sec
[bwa_aln_core] refine gapped alignments... 5.10 sec
[bwa_aln_core] print alignments... 0.54 sec
[bwa_aln_core] 9437184 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.73 sec
[bwa_aln_core] refine gapped alignments... 7.16 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 9699328 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.28 sec
[bwa_aln_core] refine gapped alignments... 5.73 sec
[bwa_aln_core] print alignments... 0.13 sec
[bwa_aln_core] 9795918 sequences have been processed.
[main] Version: 0.7.13-r1126
[main] CMD: bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532030.fastq.gz
[main] Real time: 1865.186 sec; CPU: 1837.020 sec
|
Num | 4 | ID | task.align_bwa.bwa_sam_ctl1.line_143.id_13 | Name | bwa_sam ctl1 | Thread | thread_42 | PID | 35700 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:15:38 | End | 2017-01-27 16:50:51 | Elapsed | 00:35:13 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.sai
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.flagstat.qc
| Dependencies | | |
# SYS command. line 145
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 150
bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz | samtools view -Su /dev/stdin \
| sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam
# SYS command. line 152
sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.flagstat.qc
# SYS command. line 154
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
26621 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 33.72 sec
[bwa_aln_core] refine gapped alignments... 7.03 sec
[bwa_aln_core] print alignments... [samopen] SAM header is present: 493 sequences.
0.75 sec
[bwa_aln_core] 262144 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.65 sec
[bwa_aln_core] refine gapped alignments... 7.01 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 524288 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.83 sec
[bwa_aln_core] refine gapped alignments... 6.95 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 786432 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.80 sec
[bwa_aln_core] refine gapped alignments... 6.98 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 1048576 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.64 sec
[bwa_aln_core] refine gapped alignments... 6.95 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 1310720 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.32 sec
[bwa_aln_core] refine gapped alignments... 6.89 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 1572864 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.82 sec
[bwa_aln_core] refine gapped alignments... 6.92 sec
[bwa_aln_core] print alignments... 0.73 sec
[bwa_aln_core] 1835008 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.26 sec
[bwa_aln_core] refine gapped alignments... 6.86 sec
[bwa_aln_core] print alignments... 0.72 sec
[bwa_aln_core] 2097152 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.59 sec
[bwa_aln_core] refine gapped alignments... 6.98 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 2359296 sequences have been processed.
[bwa_read_seq] 3.6% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.45 sec
[bwa_aln_core] refine gapped alignments... 6.97 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 2621440 sequences have been processed.
[bwa_read_seq] 3.5% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.42 sec
[bwa_aln_core] refine gapped alignments... 6.90 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 2883584 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.68 sec
[bwa_aln_core] refine gapped alignments... 7.06 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 3145728 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 44.71 sec
[bwa_aln_core] refine gapped alignments... 7.56 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 3407872 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 46.47 sec
[bwa_aln_core] refine gapped alignments... 8.74 sec
[bwa_aln_core] print alignments... 0.77 sec
[bwa_aln_core] 3670016 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 51.71 sec
[bwa_aln_core] refine gapped alignments... 7.83 sec
[bwa_aln_core] print alignments... 0.70 sec
[bwa_aln_core] 3932160 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 51.64 sec
[bwa_aln_core] refine gapped alignments... 9.33 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 4194304 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 50.79 sec
[bwa_aln_core] refine gapped alignments... 8.55 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 4456448 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 51.93 sec
[bwa_aln_core] refine gapped alignments... 9.46 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 4718592 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 53.16 sec
[bwa_aln_core] refine gapped alignments... 8.95 sec
[bwa_aln_core] print alignments... 0.73 sec
[bwa_aln_core] 4980736 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 53.21 sec
[bwa_aln_core] refine gapped alignments... 8.72 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 5242880 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.83 sec
[bwa_aln_core] refine gapped alignments... 7.09 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 5505024 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 54.47 sec
[bwa_aln_core] refine gapped alignments... 8.91 sec
[bwa_aln_core] print alignments... 0.67 sec
[bwa_aln_core] 5767168 sequences have been processed.
[bwa_read_seq] 3.8% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 49.48 sec
[bwa_aln_core] refine gapped alignments... 8.96 sec
[bwa_aln_core] print alignments... 0.69 sec
[bwa_aln_core] 6029312 sequences have been processed.
[bwa_read_seq] 3.6% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 49.04 sec
[bwa_aln_core] refine gapped alignments... 7.00 sec
[bwa_aln_core] print alignments... 0.72 sec
[bwa_aln_core] 6291456 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.81 sec
[bwa_aln_core] refine gapped alignments... 6.94 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 6553600 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.89 sec
[bwa_aln_core] refine gapped alignments... 7.15 sec
[bwa_aln_core] print alignments... 0.71 sec
[bwa_aln_core] 6815744 sequences have been processed.
[bwa_read_seq] 4.2% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.86 sec
[bwa_aln_core] refine gapped alignments... 7.10 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 7077888 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.26 sec
[bwa_aln_core] refine gapped alignments... 6.84 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 7340032 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.68 sec
[bwa_aln_core] refine gapped alignments... 6.99 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 7602176 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.66 sec
[bwa_aln_core] refine gapped alignments... 7.64 sec
[bwa_aln_core] print alignments... 0.76 sec
[bwa_aln_core] 7864320 sequences have been processed.
[bwa_read_seq] 4.3% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.27 sec
[bwa_aln_core] refine gapped alignments... 7.18 sec
[bwa_aln_core] print alignments... 0.74 sec
[bwa_aln_core] 8126464 sequences have been processed.
[bwa_read_seq] 4.4% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 43.52 sec
[bwa_aln_core] refine gapped alignments... 6.88 sec
[bwa_aln_core] print alignments... 0.73 sec
[bwa_aln_core] 8388608 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 42.75 sec
[bwa_aln_core] refine gapped alignments... 7.04 sec
[bwa_aln_core] print alignments... 0.75 sec
[bwa_aln_core] 8650752 sequences have been processed.
[bwa_read_seq] 4.0% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 34.56 sec
[bwa_aln_core] refine gapped alignments... 5.09 sec
[bwa_aln_core] print alignments... 0.63 sec
[bwa_aln_core] 8912896 sequences have been processed.
[bwa_read_seq] 3.9% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.13 sec
[bwa_aln_core] refine gapped alignments... 5.09 sec
[bwa_aln_core] print alignments... 0.66 sec
[bwa_aln_core] 9175040 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.07 sec
[bwa_aln_core] refine gapped alignments... 5.10 sec
[bwa_aln_core] print alignments... 0.57 sec
[bwa_aln_core] 9437184 sequences have been processed.
[bwa_read_seq] 3.7% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 31.25 sec
[bwa_aln_core] refine gapped alignments... 4.75 sec
[bwa_aln_core] print alignments... 0.66 sec
[bwa_aln_core] 9699328 sequences have been processed.
[bwa_read_seq] 4.1% bases are trimmed.
[bwa_aln_core] convert to sequence coordinate... 27.43 sec
[bwa_aln_core] refine gapped alignments... 0.35 sec
[bwa_aln_core] print alignments... 0.14 sec
[bwa_aln_core] 9795918 sequences have been processed.
[main] Version: 0.7.13-r1126
[main] CMD: bwa samse /mnt/data/bds_pipeline_genome_data/hg38/bwa_index/GRCh38.p3.genome.fa /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.sai /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/RawReadsFiles/SRR2532031.fastq.gz
[main] Real time: 1992.618 sec; CPU: 1964.088 sec
|
Num | 5 | ID | task.postalign_bam.dedup_bam_1_rep1.line_95.id_14 | Name | dedup_bam_1 rep1 | Thread | thread_41 | PID | 35770 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:45:15 | End | 2017-01-27 16:47:32 | Elapsed | 00:02:16 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam
| Dependencies | | |
# SYS command. line 97
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 105
if [[ 0 > 0 ]]; then \
sambamba sort -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.qnmsrt.bam; \
samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \
samtools view -F 1804 -Su /dev/stdin | \
sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam; \
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.qnmsrt.bam; \
else \
samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.bam | \
sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam; \
fi
# SYS command. line 116
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
5522 (process ID) old priority 0, new priority 10
|
Num | 6 | ID | task.postalign_bam.markdup_bam_picard_rep1.line_348.id_15 | Name | markdup_bam_picard rep1 | Thread | thread_41 | PID | 35774 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 1 | Mem | | | Start | 2017-01-27 16:47:33 | End | 2017-01-27 16:50:12 | Elapsed | 00:02:38 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.dup.qc
| Dependencies | | |
# SYS command. line 350
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 354
export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1"
# SYS command. line 360
if [ -f "${PICARDROOT}/picard.jar" ]; then \
MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \
elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \
MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \
elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \
MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \
fi
# SYS command. line 369
if [ -f "${MARKDUP}" ]; then \
java -Xmx4G -jar ${MARKDUP} \
INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam" \
METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.dup.qc" VALIDATION_STRINGENCY=LENIENT \
ASSUME_SORTED=true REMOVE_DUPLICATES=false; \
else \
picard MarkDuplicates \
INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam" \
METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.dup.qc" VALIDATION_STRINGENCY=LENIENT \
ASSUME_SORTED=true REMOVE_DUPLICATES=false; \
fi
# SYS command. line 381
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
27910 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1
[Fri Jan 27 16:48:07 PST 2017] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false
[Fri Jan 27 16:48:07 PST 2017] Executing as imk1@kadru on Linux 4.4.0-22-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater
INFO 2017-01-27 16:48:07 MarkDuplicates Start of doWork freeMemory: 254879584; totalMemory: 257425408; maxMemory: 11453595648
INFO 2017-01-27 16:48:07 MarkDuplicates Reading input file and constructing read end information.
INFO 2017-01-27 16:48:07 MarkDuplicates Will retain up to 44052290 data points before spilling to disk.
WARNING 2017-01-27 16:48:08 AbstractOpticalDuplicateFinderCommandLineProgram Default READ_NAME_REGEX '[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*' did not match read name 'SRR2532030.7029141'. You may need to specify a READ_NAME_REGEX in order to correctly identify optical duplicates. Note that this message will not be emitted again even if other read names do not match the regex.
INFO 2017-01-27 16:48:13 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:05s. Time for last 1,000,000: 5s. Last read position: chr2:55,418,902
INFO 2017-01-27 16:48:13 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:19 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:11s. Time for last 1,000,000: 5s. Last read position: chr3:150,758,515
INFO 2017-01-27 16:48:19 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:23 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:15s. Time for last 1,000,000: 3s. Last read position: chr5:129,044,747
INFO 2017-01-27 16:48:23 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:27 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:19s. Time for last 1,000,000: 4s. Last read position: chr7:104,553,760
INFO 2017-01-27 16:48:27 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:33 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:25s. Time for last 1,000,000: 5s. Last read position: chr10:71,964,337
INFO 2017-01-27 16:48:33 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:36 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:00:27s. Time for last 1,000,000: 2s. Last read position: chr13:45,215,780
INFO 2017-01-27 16:48:36 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:40 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:00:32s. Time for last 1,000,000: 4s. Last read position: chr17:16,378,686
INFO 2017-01-27 16:48:40 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:43 MarkDuplicates Read 8,000,000 records. Elapsed time: 00:00:35s. Time for last 1,000,000: 3s. Last read position: chrX:97,225,322
INFO 2017-01-27 16:48:43 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:48:43 MarkDuplicates Read 8165118 records. 0 pairs never matched.
INFO 2017-01-27 16:48:46 MarkDuplicates After buildSortedReadEndLists freeMemory: 1807567920; totalMemory: 2718433280; maxMemory: 11453595648
INFO 2017-01-27 16:48:46 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk.
INFO 2017-01-27 16:49:04 MarkDuplicates Traversing read pair information and detecting duplicates.
INFO 2017-01-27 16:49:04 MarkDuplicates Traversing fragment information and detecting duplicates.
INFO 2017-01-27 16:49:05 MarkDuplicates Sorting list of duplicate records.
INFO 2017-01-27 16:49:06 MarkDuplicates After generateDuplicateIndexes freeMemory: 2682879952; totalMemory: 5582094336; maxMemory: 11453595648
INFO 2017-01-27 16:49:06 MarkDuplicates Marking 370943 records as duplicates.
INFO 2017-01-27 16:49:06 MarkDuplicates Found 0 optical duplicate clusters.
INFO 2017-01-27 16:50:09 MarkDuplicates Before output close freeMemory: 6074273224; totalMemory: 6123159552; maxMemory: 11453595648
INFO 2017-01-27 16:50:10 MarkDuplicates After output close freeMemory: 6135613760; totalMemory: 6184501248; maxMemory: 11453595648
[Fri Jan 27 16:50:10 PST 2017] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 2.06 minutes.
Runtime.totalMemory()=6184501248
|
Num | 7 | ID | task.postalign_bam.dedup_bam_2_rep1.line_144.id_16 | Name | dedup_bam_2 rep1 | Thread | thread_41 | PID | 35779 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:50:13 | End | 2017-01-27 16:52:48 | Elapsed | 00:02:34 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.flagstat.qc
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.pbc.qc
| Dependencies | | |
# SYS command. line 146
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 150
samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam
# SYS command. line 153
sambamba index -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam
# SYS command. line 155
sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.flagstat.qc
# SYS command. line 165
bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.dupmark.bam | \
awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \
grep -v 'chrM' | sort | uniq -c | \
awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.pbc.qc
# SYS command. line 169
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
29933 (process ID) old priority 0, new priority 10
|
Num | 8 | ID | task.postalign_bam.dedup_bam_1_ctl1.line_95.id_17 | Name | dedup_bam_1 ctl1 | Thread | thread_42 | PID | 35780 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:50:53 | End | 2017-01-27 16:52:52 | Elapsed | 00:01:58 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam
| Dependencies | | |
# SYS command. line 97
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 105
if [[ 0 > 0 ]]; then \
sambamba sort -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam -n -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.qnmsrt.bam; \
samtools view -h /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.qnmsrt.bam | $(which assign_multimappers.py) -k 0 | \
samtools view -F 1804 -Su /dev/stdin | \
sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam; \
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.qnmsrt.bam; \
else \
samtools view -F 1804 -q 30 -u /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.bam | \
sambamba sort -t 2 /dev/stdin -o /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam; \
fi
# SYS command. line 116
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
33297 (process ID) old priority 0, new priority 10
|
Num | 9 | ID | task.postalign_bam.bam_to_tag_rep1.line_505.id_18 | Name | bam_to_tag rep1 | Thread | thread_41 | PID | 35782 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 16:52:49 | End | 2017-01-27 16:54:07 | Elapsed | 00:01:18 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz
| Dependencies | | |
# SYS command. line 507
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 510
bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz
# SYS command. line 512
echo
# SYS command. line 514
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
28439 (process ID) old priority 0, new priority 10
Waiting for 42 seconds.
|
Num | 10 | ID | task.postalign_bam.markdup_bam_picard_ctl1.line_348.id_19 | Name | markdup_bam_picard ctl1 | Thread | thread_42 | PID | 35783 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 1 | Mem | | | Start | 2017-01-27 16:52:53 | End | 2017-01-27 16:55:03 | Elapsed | 00:02:10 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.dup.qc
| Dependencies | | |
# SYS command. line 350
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 354
export _JAVA_OPTIONS="-Xms256M -Xmx12G -XX:ParallelGCThreads=1"
# SYS command. line 360
if [ -f "${PICARDROOT}/picard.jar" ]; then \
MARKDUP="${PICARDROOT}/picard.jar MarkDuplicates"; \
elif [ -f "${PICARDROOT}/MarkDuplicates.jar" ]; then \
MARKDUP="${PICARDROOT}/MarkDuplicates.jar"; \
elif [ $(which picard 2> /dev/null | wc -l || echo) == "1" ]; then \
MARKDUP="$(ls $(dirname $(which picard))/../share/picard*/MarkDuplicates.jar 2> /dev/null || echo)"; \
fi
# SYS command. line 369
if [ -f "${MARKDUP}" ]; then \
java -Xmx4G -jar ${MARKDUP} \
INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam" \
METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.dup.qc" VALIDATION_STRINGENCY=LENIENT \
ASSUME_SORTED=true REMOVE_DUPLICATES=false; \
else \
picard MarkDuplicates \
INPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam" OUTPUT="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam" \
METRICS_FILE="/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.dup.qc" VALIDATION_STRINGENCY=LENIENT \
ASSUME_SORTED=true REMOVE_DUPLICATES=false; \
fi
# SYS command. line 381
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
28438 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
Picked up _JAVA_OPTIONS: -Xms256M -Xmx12G -XX:ParallelGCThreads=1
[Fri Jan 27 16:53:07 PST 2017] picard.sam.markduplicates.MarkDuplicates INPUT=[/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.filt.bam] OUTPUT=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam METRICS_FILE=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.dup.qc REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false
[Fri Jan 27 16:53:07 PST 2017] Executing as imk1@kadru on Linux 4.4.0-22-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_92-b15; Picard version: 1.126(4691ee611ac205d4afe2a1b7a2ea975a6f997426_1417447214) IntelDeflater
INFO 2017-01-27 16:53:07 MarkDuplicates Start of doWork freeMemory: 254879728; totalMemory: 257425408; maxMemory: 11453595648
INFO 2017-01-27 16:53:07 MarkDuplicates Reading input file and constructing read end information.
INFO 2017-01-27 16:53:07 MarkDuplicates Will retain up to 44052290 data points before spilling to disk.
WARNING 2017-01-27 16:53:07 AbstractOpticalDuplicateFinderCommandLineProgram Default READ_NAME_REGEX '[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*' did not match read name 'SRR2532031.7594466'. You may need to specify a READ_NAME_REGEX in order to correctly identify optical duplicates. Note that this message will not be emitted again even if other read names do not match the regex.
INFO 2017-01-27 16:53:12 MarkDuplicates Read 1,000,000 records. Elapsed time: 00:00:04s. Time for last 1,000,000: 4s. Last read position: chr2:84,259,537
INFO 2017-01-27 16:53:12 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:16 MarkDuplicates Read 2,000,000 records. Elapsed time: 00:00:08s. Time for last 1,000,000: 4s. Last read position: chr3:189,530,251
INFO 2017-01-27 16:53:16 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:21 MarkDuplicates Read 3,000,000 records. Elapsed time: 00:00:13s. Time for last 1,000,000: 5s. Last read position: chr6:3,215,105
INFO 2017-01-27 16:53:21 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:25 MarkDuplicates Read 4,000,000 records. Elapsed time: 00:00:17s. Time for last 1,000,000: 3s. Last read position: chr8:33,724,508
INFO 2017-01-27 16:53:25 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:28 MarkDuplicates Read 5,000,000 records. Elapsed time: 00:00:21s. Time for last 1,000,000: 3s. Last read position: chr11:22,120,616
INFO 2017-01-27 16:53:28 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:33 MarkDuplicates Read 6,000,000 records. Elapsed time: 00:00:25s. Time for last 1,000,000: 4s. Last read position: chr13:103,292,887
INFO 2017-01-27 16:53:33 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:36 MarkDuplicates Read 7,000,000 records. Elapsed time: 00:00:28s. Time for last 1,000,000: 2s. Last read position: chr19:44,908,075
INFO 2017-01-27 16:53:36 MarkDuplicates Tracking 0 as yet unmatched pairs. 0 records in RAM.
INFO 2017-01-27 16:53:39 MarkDuplicates Read 7759676 records. 0 pairs never matched.
INFO 2017-01-27 16:53:41 MarkDuplicates After buildSortedReadEndLists freeMemory: 1609222328; totalMemory: 2489319424; maxMemory: 11453595648
INFO 2017-01-27 16:53:41 MarkDuplicates Will retain up to 357924864 duplicate indices before spilling to disk.
INFO 2017-01-27 16:54:00 MarkDuplicates Traversing read pair information and detecting duplicates.
INFO 2017-01-27 16:54:00 MarkDuplicates Traversing fragment information and detecting duplicates.
INFO 2017-01-27 16:54:00 MarkDuplicates Sorting list of duplicate records.
INFO 2017-01-27 16:54:02 MarkDuplicates After generateDuplicateIndexes freeMemory: 2462256088; totalMemory: 5356650496; maxMemory: 11453595648
INFO 2017-01-27 16:54:02 MarkDuplicates Marking 104979 records as duplicates.
INFO 2017-01-27 16:54:02 MarkDuplicates Found 0 optical duplicate clusters.
INFO 2017-01-27 16:55:00 MarkDuplicates Before output close freeMemory: 5639662344; totalMemory: 5679087616; maxMemory: 11453595648
INFO 2017-01-27 16:55:02 MarkDuplicates After output close freeMemory: 5705197184; totalMemory: 5744623616; maxMemory: 11453595648
[Fri Jan 27 16:55:02 PST 2017] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 1.93 minutes.
Runtime.totalMemory()=5744623616
|
Num | 11 | ID | task.postalign_bed.subsample_tag_rep1.line_45.id_20 | Name | subsample_tag rep1 | Thread | thread_41 | PID | 35785 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 16:54:09 | End | 2017-01-27 16:55:22 | Elapsed | 00:01:13 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz
| Dependencies | | |
# SYS command. line 47
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 50
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | \
grep -v "chrM" | shuf -n 15000000 --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz
# SYS command. line 53
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
28698 (process ID) old priority 0, new priority 10
Waiting for 45 seconds.
|
Num | 12 | ID | task.postalign_bed.spr_rep1.line_255.id_21 | Name | spr rep1 | Thread | thread_41 | PID | 35786 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 16:54:09 | End | 2017-01-27 16:55:23 | Elapsed | 00:01:13 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz
| Dependencies | | |
# SYS command. line 257
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 260
nlines=$( zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | wc -l )
# SYS command. line 261
nlines=$(( (nlines + 1) / 2 ))
# SYS command. line 265
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | shuf --random-source=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | split -d -l $((nlines)) - /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.
# SYS command. line 268
gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.00 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz
# SYS command. line 269
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.00
# SYS command. line 270
gzip -nc /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.01 > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz
# SYS command. line 271
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.01
# SYS command. line 273
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
28699 (process ID) old priority 0, new priority 10
Waiting for 39 seconds.
|
Num | 13 | ID | task.postalign_bam.dedup_bam_2_ctl1.line_144.id_22 | Name | dedup_bam_2 ctl1 | Thread | thread_42 | PID | 35787 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:55:05 | End | 2017-01-27 16:57:21 | Elapsed | 00:02:16 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.nodup.flagstat.qc
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.nodup.pbc.qc
| Dependencies | | |
# SYS command. line 146
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 150
samtools view -F 1804 -b /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam
# SYS command. line 153
sambamba index -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam
# SYS command. line 155
sambamba flagstat -t 2 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.nodup.flagstat.qc
# SYS command. line 165
bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.dupmark.bam | \
awk 'BEGIN{OFS="\t"}{print $1,$2,$3,$6}' | \
grep -v 'chrM' | sort | uniq -c | \
awk 'BEGIN{mt=0;m0=0;m1=0;m2=0} ($1==1){m1=m1+1} ($1==2){m2=m2+1} {m0=m0+1} {mt=mt+$1} END{m1_m2=-1.0; if(m2>0) m1_m2=m1/m2; printf "%d\t%d\t%d\t%d\t%f\t%f\t%f\n",mt,m0,m1,m2,m0/mt,m1/m0,m1_m2}' > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/ctl1/SRR2532031.nodup.pbc.qc
# SYS command. line 169
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
55947 (process ID) old priority 0, new priority 10
|
Num | 14 | ID | task.postalign_bed.xcor_rep1.line_212.id_23 | Name | xcor rep1 | Thread | thread_41 | PID | 35788 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | 2 | Mem | | | Start | 2017-01-27 16:55:24 | End | 2017-01-27 17:01:36 | Elapsed | 00:06:11 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.pdf
| Dependencies | | |
# SYS command. line 214
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 217
if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R); \
else RUN_SPP=$(which run_spp.R); \
fi
# SYS command. line 223
Rscript ${RUN_SPP} -rf \
-c=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz -p=2 \
-filtchr=chrM -savp=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.pdf -out=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc
# SYS command. line 226
sed -r 's/,[^\t]+//g' /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc.tmp
# SYS command. line 227
mv /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc.tmp /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc
# SYS command. line 229
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
728 (process ID) old priority 0, new priority 10
################
ChIP data: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz
Control data: NA
strandshift(min): -500
strandshift(step): 5
strandshift(max) 1500
user-defined peak shift NA
exclusion(min): 10
exclusion(max): NaN
num parallel nodes: 2
FDR threshold: 0.01
NumPeaks Threshold: NA
Output Directory: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1
narrowPeak output file name: NA
regionPeak output file name: NA
Rdata filename: NA
plot pdf filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.pdf
result filename: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.qc
Overwrite files?: TRUE
Decompressing ChIP file
Reading ChIP tagAlign/BAM file /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.15M.tagAlign.gz
opened /tmp/35788.1.q/RtmpURA8oC/SRR2532030.nodup.15M.tagAlign95d79e9988c
done. read 7792886 fragments
ChIP data read length 49
[1] TRUE
Calculating peak characteristics
Minimum cross-correlation value 0.09973635
Minimum cross-correlation shift 1500
Top 3 cross-correlation values 0.110809746195814,0.104580474718593,0.104543193996282
Top 3 estimates for fragment length 150,285,320
Window half size 415
Phantom peak location 40
Phantom peak Correlation 0.1065764
Normalized Strand cross-correlation coefficient (NSC) 1.111027
Relative Strand cross-correlation Coefficient (RSC) 1.618914
Phantom Peak Quality Tag 2
null device
1
--------------------Stderr--------------------
Loading required package: caTools
|
Num | 15 | ID | task.postalign_bam.bam_to_tag_ctl1.line_505.id_24 | Name | bam_to_tag ctl1 | Thread | thread_42 | PID | 35792 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 16:57:23 | End | 2017-01-27 16:58:37 | Elapsed | 00:01:13 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz
| Dependencies | | |
# SYS command. line 507
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 510
bedtools bamtobed -i /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.bam | awk 'BEGIN{OFS="\t"}{$4="N";$5="1000";print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz
# SYS command. line 512
echo
# SYS command. line 514
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
29055 (process ID) old priority 0, new priority 10
Waiting for 40 seconds.
|
Num | 16 | ID | task.callpeak_macs2.macs2_rep1.line_71.id_25 | Name | macs2 rep1 | Thread | thread_Root | PID | 35796 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:01:38 | End | 2017-01-27 17:15:26 | Elapsed | 00:13:48 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/signal/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bw
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/signal/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bw
| Dependencies | | |
# SYS command. line 73
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 74
export LC_COLLATE=C
# SYS command. line 79
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# SYS command. line 82
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
# SYS command. line 85
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 92
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# SYS command. line 95
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
# SYS command. line 96
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
# SYS command. line 99
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 110
if [[ true == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 121
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1 -o "SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 124
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 125
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg
# SYS command. line 128
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 129
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/signal/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bw
# SYS command. line 130
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 136
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 138
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 140
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1 -o "SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 143
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 144
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg
# SYS command. line 147
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 148
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/signal/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bw
# SYS command. line 149
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 151
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 153
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
29346 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
INFO @ Fri, 27 Jan 2017 17:01:54:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Fri, 27 Jan 2017 17:01:54: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:01:54: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:01:56: 1000000
INFO @ Fri, 27 Jan 2017 17:01:57: 2000000
INFO @ Fri, 27 Jan 2017 17:01:59: 3000000
INFO @ Fri, 27 Jan 2017 17:02:00: 4000000
INFO @ Fri, 27 Jan 2017 17:02:02: 5000000
INFO @ Fri, 27 Jan 2017 17:02:03: 6000000
INFO @ Fri, 27 Jan 2017 17:02:05: 7000000
INFO @ Fri, 27 Jan 2017 17:02:06: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:02:08: 1000000
INFO @ Fri, 27 Jan 2017 17:02:09: 2000000
INFO @ Fri, 27 Jan 2017 17:02:10: 3000000
INFO @ Fri, 27 Jan 2017 17:02:12: 4000000
INFO @ Fri, 27 Jan 2017 17:02:13: 5000000
INFO @ Fri, 27 Jan 2017 17:02:15: 6000000
INFO @ Fri, 27 Jan 2017 17:02:16: 7000000
INFO @ Fri, 27 Jan 2017 17:02:17: #1 tag size is determined as 49 bps
INFO @ Fri, 27 Jan 2017 17:02:17: #1 tag size = 49
INFO @ Fri, 27 Jan 2017 17:02:17: #1 total tags in treatment: 7794175
INFO @ Fri, 27 Jan 2017 17:02:17: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:02:17: #1 finished!
INFO @ Fri, 27 Jan 2017 17:02:17: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:02:17: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:02:17: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:02:17: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:02:17: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Fri, 27 Jan 2017 17:02:17: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:02:47: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Fri, 27 Jan 2017 17:02:47: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_treat_pileup.bdg
INFO @ Fri, 27 Jan 2017 17:02:47: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_control_lambda.bdg
INFO @ Fri, 27 Jan 2017 17:02:47: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Fri, 27 Jan 2017 17:02:47: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:04:06: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:04:07: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.narrowPeak
INFO @ Fri, 27 Jan 2017 17:04:08: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_summits.bed
INFO @ Fri, 27 Jan 2017 17:04:08: Done!
INFO @ Fri, 27 Jan 2017 17:04:10:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/rep1/SRR2532030.nodup.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Fri, 27 Jan 2017 17:04:10: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:04:10: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:04:12: 1000000
INFO @ Fri, 27 Jan 2017 17:04:14: 2000000
INFO @ Fri, 27 Jan 2017 17:04:15: 3000000
INFO @ Fri, 27 Jan 2017 17:04:17: 4000000
INFO @ Fri, 27 Jan 2017 17:04:18: 5000000
INFO @ Fri, 27 Jan 2017 17:04:20: 6000000
INFO @ Fri, 27 Jan 2017 17:04:21: 7000000
INFO @ Fri, 27 Jan 2017 17:04:23: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:04:24: 1000000
INFO @ Fri, 27 Jan 2017 17:04:26: 2000000
INFO @ Fri, 27 Jan 2017 17:04:27: 3000000
INFO @ Fri, 27 Jan 2017 17:04:29: 4000000
INFO @ Fri, 27 Jan 2017 17:04:30: 5000000
INFO @ Fri, 27 Jan 2017 17:04:32: 6000000
INFO @ Fri, 27 Jan 2017 17:04:33: 7000000
INFO @ Fri, 27 Jan 2017 17:04:35: #1 tag size is determined as 49 bps
INFO @ Fri, 27 Jan 2017 17:04:35: #1 tag size = 49
INFO @ Fri, 27 Jan 2017 17:04:35: #1 total tags in treatment: 7794175
INFO @ Fri, 27 Jan 2017 17:04:35: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:04:35: #1 finished!
INFO @ Fri, 27 Jan 2017 17:04:35: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:04:35: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:04:35: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:04:35: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:04:35: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Fri, 27 Jan 2017 17:04:35: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:05:04: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:05:27: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:05:30: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.broadPeak
INFO @ Fri, 27 Jan 2017 17:05:31: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.gappedPeak
INFO @ Fri, 27 Jan 2017 17:05:33: Done!
INFO @ Fri, 27 Jan 2017 17:05:40: Read and build treatment bedGraph...
INFO @ Fri, 27 Jan 2017 17:05:55: Read and build control bedGraph...
INFO @ Fri, 27 Jan 2017 17:06:17: Build scoreTrackII...
INFO @ Fri, 27 Jan 2017 17:06:29: Calculate scores comparing treatment and control by 'FE'...
INFO @ Fri, 27 Jan 2017 17:07:53: Write bedGraph of scores...
INFO @ Fri, 27 Jan 2017 17:08:34: Finished 'FE'! Please check '/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_FE.bdg'!
INFO @ Fri, 27 Jan 2017 17:10:16: Read and build treatment bedGraph...
INFO @ Fri, 27 Jan 2017 17:10:30: Read and build control bedGraph...
INFO @ Fri, 27 Jan 2017 17:10:52: Build scoreTrackII...
INFO @ Fri, 27 Jan 2017 17:11:04: Values in your input bedGraph files will be multiplied by 7.654697 ...
INFO @ Fri, 27 Jan 2017 17:12:26: Calculate scores comparing treatment and control by 'ppois'...
INFO @ Fri, 27 Jan 2017 17:12:37: Write bedGraph of scores...
INFO @ Fri, 27 Jan 2017 17:13:18: Finished 'ppois'! Please check '/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign_ppois.bdg'!
|
Num | 17 | ID | task.callpeak_macs2.macs2_rep1_pr1.line_71.id_26 | Name | macs2 rep1-pr1 | Thread | thread_Root | PID | 35797 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:01:39 | End | 2017-01-27 17:05:56 | Elapsed | 00:04:16 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
| Dependencies | | |
# SYS command. line 73
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 74
export LC_COLLATE=C
# SYS command. line 79
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# SYS command. line 82
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
# SYS command. line 85
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 92
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# SYS command. line 95
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
# SYS command. line 96
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
# SYS command. line 99
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 110
if [[ false == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 121
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1 -o "SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 124
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 125
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg
# SYS command. line 128
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 129
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bw
# SYS command. line 130
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 136
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 138
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 140
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1 -o "SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 143
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 144
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg
# SYS command. line 147
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 148
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bw
# SYS command. line 149
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 151
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 153
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
29347 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
INFO @ Fri, 27 Jan 2017 17:01:54:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Fri, 27 Jan 2017 17:01:54: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:01:54: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:01:56: 1000000
INFO @ Fri, 27 Jan 2017 17:01:58: 2000000
INFO @ Fri, 27 Jan 2017 17:01:59: 3000000
INFO @ Fri, 27 Jan 2017 17:02:01: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:02:03: 1000000
INFO @ Fri, 27 Jan 2017 17:02:04: 2000000
INFO @ Fri, 27 Jan 2017 17:02:06: 3000000
INFO @ Fri, 27 Jan 2017 17:02:07: 4000000
INFO @ Fri, 27 Jan 2017 17:02:09: 5000000
INFO @ Fri, 27 Jan 2017 17:02:10: 6000000
INFO @ Fri, 27 Jan 2017 17:02:11: 7000000
INFO @ Fri, 27 Jan 2017 17:02:13: #1 tag size is determined as 49 bps
INFO @ Fri, 27 Jan 2017 17:02:13: #1 tag size = 49
INFO @ Fri, 27 Jan 2017 17:02:13: #1 total tags in treatment: 3897088
INFO @ Fri, 27 Jan 2017 17:02:13: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:02:13: #1 finished!
INFO @ Fri, 27 Jan 2017 17:02:13: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:02:13: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:02:13: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:02:13: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:02:13: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Fri, 27 Jan 2017 17:02:13: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:02:38: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Fri, 27 Jan 2017 17:02:38: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_treat_pileup.bdg
INFO @ Fri, 27 Jan 2017 17:02:38: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_control_lambda.bdg
INFO @ Fri, 27 Jan 2017 17:02:38: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Fri, 27 Jan 2017 17:02:38: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:03:46: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:03:47: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.narrowPeak
INFO @ Fri, 27 Jan 2017 17:03:48: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_summits.bed
INFO @ Fri, 27 Jan 2017 17:03:48: Done!
INFO @ Fri, 27 Jan 2017 17:03:52:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Fri, 27 Jan 2017 17:03:52: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:03:52: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:03:54: 1000000
INFO @ Fri, 27 Jan 2017 17:03:56: 2000000
INFO @ Fri, 27 Jan 2017 17:03:57: 3000000
INFO @ Fri, 27 Jan 2017 17:03:59: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:04:01: 1000000
INFO @ Fri, 27 Jan 2017 17:04:02: 2000000
INFO @ Fri, 27 Jan 2017 17:04:03: 3000000
INFO @ Fri, 27 Jan 2017 17:04:05: 4000000
INFO @ Fri, 27 Jan 2017 17:04:07: 5000000
INFO @ Fri, 27 Jan 2017 17:04:08: 6000000
INFO @ Fri, 27 Jan 2017 17:04:10: 7000000
INFO @ Fri, 27 Jan 2017 17:04:11: #1 tag size is determined as 49 bps
INFO @ Fri, 27 Jan 2017 17:04:11: #1 tag size = 49
INFO @ Fri, 27 Jan 2017 17:04:11: #1 total tags in treatment: 3897088
INFO @ Fri, 27 Jan 2017 17:04:11: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:04:11: #1 finished!
INFO @ Fri, 27 Jan 2017 17:04:11: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:04:11: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:04:11: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:04:11: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:04:11: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Fri, 27 Jan 2017 17:04:11: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:04:36: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:05:23: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:05:31: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.broadPeak
INFO @ Fri, 27 Jan 2017 17:05:36: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.gappedPeak
INFO @ Fri, 27 Jan 2017 17:05:41: Done!
|
Num | 18 | ID | task.callpeak_macs2.macs2_rep1_pr2.line_71.id_27 | Name | macs2 rep1-pr2 | Thread | thread_Root | PID | 35798 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:01:41 | End | 2017-01-27 17:06:23 | Elapsed | 00:04:41 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
| Dependencies | | |
# SYS command. line 73
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 74
export LC_COLLATE=C
# SYS command. line 79
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# SYS command. line 82
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
# SYS command. line 85
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.narrowPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 92
macs2 callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# SYS command. line 95
sort -k 8gr,8gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
# SYS command. line 96
sort -k 14gr,14gr "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak | awk 'BEGIN{OFS="\t"}{$4="Peak_"NR ; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
# SYS command. line 99
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.xls \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.broadPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_peaks.gappedPeak \
"/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_summits.bed
# SYS command. line 110
if [[ false == "false" ]]; then \
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg; \
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi; \
exit; \
fi
# SYS command. line 121
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2 -o "SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -m FE
# SYS command. line 124
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph
# SYS command. line 125
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_FE.bdg
# SYS command. line 128
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 129
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bw
# SYS command. line 130
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.fc.signal.srt.bedgraph
# SYS command. line 136
chipReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}')
# SYS command. line 138
controlReads=$(zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz | wc -l | awk '{printf "%f", $1/1000000}'); sval=$(echo "${chipReads} ${controlReads}" | awk '$1>$2{printf "%f",$2} $1<=$2{printf "%f",$1}')
# SYS command. line 140
macs2 bdgcmp -t "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg -c "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg --outdir /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2 -o "SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -m ppois -S "${sval}"
# SYS command. line 143
slopBed -i "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg -g /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes -b 0 | awk '{if ($3 != -1) print $0}' | bedClip stdin /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph
# SYS command. line 144
rm -rf "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_ppois.bdg
# SYS command. line 147
sort -k1,1 -k2,2n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 148
bedGraphToBigWig /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph /mnt/data/bds_pipeline_genome_data/hg38/hg38.chrom.sizes /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bw
# SYS command. line 149
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.bedgraph /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.pval.signal.srt.bedgraph
# SYS command. line 151
rm -f "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_treat_pileup.bdg "/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign"_control_lambda.bdg
# SYS command. line 153
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
27950 (process ID) old priority 0, new priority 10
--------------------Stderr--------------------
INFO @ Fri, 27 Jan 2017 17:02:06:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --nomodel --shift 0 --extsize 150 --keep-dup all -B --SPMR
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff = 1.00e-02
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is off
# MACS will save fragment pileup signal per million reads
INFO @ Fri, 27 Jan 2017 17:02:06: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:02:06: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:02:08: 1000000
INFO @ Fri, 27 Jan 2017 17:02:09: 2000000
INFO @ Fri, 27 Jan 2017 17:02:11: 3000000
INFO @ Fri, 27 Jan 2017 17:02:12: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:02:14: 1000000
INFO @ Fri, 27 Jan 2017 17:02:16: 2000000
INFO @ Fri, 27 Jan 2017 17:02:17: 3000000
INFO @ Fri, 27 Jan 2017 17:02:19: 4000000
INFO @ Fri, 27 Jan 2017 17:02:20: 5000000
INFO @ Fri, 27 Jan 2017 17:02:22: 6000000
INFO @ Fri, 27 Jan 2017 17:02:23: 7000000
INFO @ Fri, 27 Jan 2017 17:02:24: #1 tag size is determined as 47 bps
INFO @ Fri, 27 Jan 2017 17:02:24: #1 tag size = 47
INFO @ Fri, 27 Jan 2017 17:02:24: #1 total tags in treatment: 3897087
INFO @ Fri, 27 Jan 2017 17:02:24: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:02:24: #1 finished!
INFO @ Fri, 27 Jan 2017 17:02:24: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:02:24: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:02:24: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:02:24: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:02:24: #3 Call peaks with given -log10pvalue cutoff: 2.00000 ...
INFO @ Fri, 27 Jan 2017 17:02:24: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:02:55: #3 In the peak calling step, the following will be performed simultaneously:
INFO @ Fri, 27 Jan 2017 17:02:55: #3 Write bedGraph files for treatment pileup (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_treat_pileup.bdg
INFO @ Fri, 27 Jan 2017 17:02:55: #3 Write bedGraph files for control lambda (after scaling if necessary)... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_control_lambda.bdg
INFO @ Fri, 27 Jan 2017 17:02:55: #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads.
INFO @ Fri, 27 Jan 2017 17:02:55: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:04:02: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:04:03: #4 Write peak in narrowPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.narrowPeak
INFO @ Fri, 27 Jan 2017 17:04:03: #4 Write summits bed file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_summits.bed
INFO @ Fri, 27 Jan 2017 17:04:04: Done!
INFO @ Fri, 27 Jan 2017 17:04:08:
# Command line: callpeak -t /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz -c /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz -f BED -n /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign -g hs -p 0.01 --broad --nomodel --shift 0 --extsize 150 --keep-dup all
# ARGUMENTS LIST:
# name = /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign
# format = BED
# ChIP-seq file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign.gz']
# control file = ['/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/align/ctl1/SRR2532031.nodup.tagAlign.gz']
# effective genome size = 2.70e+09
# band width = 300
# model fold = [5, 50]
# pvalue cutoff for narrow/strong regions = 1.00e-02
# pvalue cutoff for broad/weak regions = 1.00e-01
# qvalue will not be calculated and reported as -1 in the final output.
# Larger dataset will be scaled towards smaller dataset.
# Range for calculating regional lambda is: 1000 bps and 10000 bps
# Broad region calling is on
INFO @ Fri, 27 Jan 2017 17:04:08: #1 read tag files...
INFO @ Fri, 27 Jan 2017 17:04:08: #1 read treatment tags...
INFO @ Fri, 27 Jan 2017 17:04:09: 1000000
INFO @ Fri, 27 Jan 2017 17:04:11: 2000000
INFO @ Fri, 27 Jan 2017 17:04:13: 3000000
INFO @ Fri, 27 Jan 2017 17:04:14: #1.2 read input tags...
INFO @ Fri, 27 Jan 2017 17:04:16: 1000000
INFO @ Fri, 27 Jan 2017 17:04:17: 2000000
INFO @ Fri, 27 Jan 2017 17:04:19: 3000000
INFO @ Fri, 27 Jan 2017 17:04:20: 4000000
INFO @ Fri, 27 Jan 2017 17:04:22: 5000000
INFO @ Fri, 27 Jan 2017 17:04:23: 6000000
INFO @ Fri, 27 Jan 2017 17:04:25: 7000000
INFO @ Fri, 27 Jan 2017 17:04:26: #1 tag size is determined as 47 bps
INFO @ Fri, 27 Jan 2017 17:04:26: #1 tag size = 47
INFO @ Fri, 27 Jan 2017 17:04:26: #1 total tags in treatment: 3897087
INFO @ Fri, 27 Jan 2017 17:04:26: #1 total tags in control: 7654697
INFO @ Fri, 27 Jan 2017 17:04:26: #1 finished!
INFO @ Fri, 27 Jan 2017 17:04:26: #2 Build Peak Model...
INFO @ Fri, 27 Jan 2017 17:04:26: #2 Skipped...
INFO @ Fri, 27 Jan 2017 17:04:26: #2 Use 150 as fragment length
INFO @ Fri, 27 Jan 2017 17:04:26: #3 Call peaks...
INFO @ Fri, 27 Jan 2017 17:04:26: #3 Call broad peaks with given level1 -log10pvalue cutoff and level2: 2.00000, 1.00000...
INFO @ Fri, 27 Jan 2017 17:04:26: #3 Pre-compute pvalue-qvalue table...
INFO @ Fri, 27 Jan 2017 17:05:02: #3 Call peaks for each chromosome...
INFO @ Fri, 27 Jan 2017 17:05:53: #4 Write output xls file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.xls
INFO @ Fri, 27 Jan 2017 17:06:00: #4 Write broad peak in broadPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.broadPeak
INFO @ Fri, 27 Jan 2017 17:06:04: #4 Write broad peak in bed12/gappedPeak format file... /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign_peaks.gappedPeak
INFO @ Fri, 27 Jan 2017 17:06:08: Done!
|
Num | 19 | ID | task.callpeak_naive_overlap.naive_overlap_thresh.line_59.id_28 | Name | naive_overlap_thresh | Thread | thread_Root | PID | 35811 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:15:28 | End | 2017-01-27 17:16:53 | Elapsed | 00:01:25 | Timeout | 01:00:00 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.gz
| Dependencies | | |
# SYS command. line 61
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 64
intersectBed -wo -a <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz) -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$13-$12; if (($21/s1 >= 0.5) || ($21/s2 >= 0.5)) {print $0}}' | cut -f 1-10 | sort | uniq | \
intersectBed -wo -a stdin -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$13-$12; if (($21/s1 >= 0.5) || ($21/s2 >= 0.5)) {print $0}}' | cut -f 1-10 | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.narrowPeak.gz
# SYS command. line 70
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.narrowPeak.gz | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.narrowPeak.gz
# SYS command. line 72
bedtools intersect -v -a /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.narrowPeak.gz -b /mnt/data/annotations/by_release/hg20.GRCh38/hg38.blacklist.bed.gz | grep -P 'chr[\dXY]+[ \t]' | awk 'BEGIN{OFS="\t"} {if ($5>1000) $5=1000; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.gz
# SYS command. line 74
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.narrowPeak.gz
# SYS command. line 76
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
30686 (process ID) old priority 0, new priority 10
Waiting for 57 seconds.
--------------------Stderr--------------------
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
GL000205.2 140187 140337 Peak_26831 41 . 3.28811 4.11329 1.27103 22
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
GL000205.2 140187 140337 Peak_26831 41 . 3.28811 4.11329 1.27103 22
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
GL383557.1 54711 54863 Peak_16100 54 . 4.11014 5.47999 2.41770 49
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
GL383557.1 54711 54863 Peak_16100 54 . 4.11014 5.47999 2.41770 49
|
Num | 20 | ID | task.callpeak_naive_overlap.naive_overlap_thresh.line_59.id_29 | Name | naive_overlap_thresh | Thread | thread_Root | PID | 35812 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:15:28 | End | 2017-01-27 17:16:52 | Elapsed | 00:01:24 | Timeout | 01:00:00 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.gz
| Dependencies | | |
# SYS command. line 61
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 64
intersectBed -wo -a <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz) -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$18-$17; if (($31/s1 >= 0.5) || ($31/s2 >= 0.5)) {print $0}}' | cut -f 1-15 | sort | uniq | \
intersectBed -wo -a stdin -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.gappedPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$18-$17; if (($31/s1 >= 0.5) || ($31/s2 >= 0.5)) {print $0}}' | cut -f 1-15 | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.gappedPeak.gz
# SYS command. line 70
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.gappedPeak.gz | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.gappedPeak.gz
# SYS command. line 72
bedtools intersect -v -a /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.gappedPeak.gz -b /mnt/data/annotations/by_release/hg20.GRCh38/hg38.blacklist.bed.gz | grep -P 'chr[\dXY]+[ \t]' | awk 'BEGIN{OFS="\t"} {if ($5>1000) $5=1000; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.gz
# SYS command. line 74
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.gappedPeak.gz
# SYS command. line 76
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
30685 (process ID) old priority 0, new priority 10
Waiting for 43 seconds.
--------------------Stderr--------------------
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
KI270772.1 80077 80302 Peak_43315 5 . 80077 80302 0 3 1,150,1 0,4,224 2.15378 2.49792 0.51405
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
KI270772.1 80077 80302 Peak_43315 5 . 80077 80302 0 3 1,150,1 0,4,224 2.15378 2.49792 0.51405
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
KI270830.1 14303 17326 Peak_96230 2 . 14303 17326 0 4 1,150,150,1 0,12,1591,3022 1.60466 1.71608 0.21683
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
KI270830.1 14303 17326 Peak_96230 2 . 14303 17326 0 4 1,150,150,1 0,12,1591,3022 1.60466 1.71608 0.21683
|
Num | 21 | ID | task.callpeak_naive_overlap.naive_overlap_thresh.line_59.id_30 | Name | naive_overlap_thresh | Thread | thread_Root | PID | 35813 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:15:29 | End | 2017-01-27 17:16:54 | Elapsed | 00:01:25 | Timeout | 01:00:00 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.broadPeak.gz
| Dependencies | | |
# SYS command. line 61
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 64
intersectBed -wo -a <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz) -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr1/SRR2532030.nodup.pr1.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$12-$11; if (($19/s1 >= 0.5) || ($19/s2 >= 0.5)) {print $0}}' | cut -f 1-9 | sort | uniq | \
intersectBed -wo -a stdin -b <(zcat -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/pseudo_reps/rep1/pr2/SRR2532030.nodup.pr2.tagAlign_x_SRR2532031.nodup.tagAlign.broadPeak.gz) | \
awk 'BEGIN{FS="\t";OFS="\t"} {s1=$3-$2; s2=$12-$11; if (($19/s1 >= 0.5) || ($19/s2 >= 0.5)) {print $0}}' | cut -f 1-9 | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.broadPeak.gz
# SYS command. line 70
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.broadPeak.gz | sort | uniq | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.broadPeak.gz
# SYS command. line 72
bedtools intersect -v -a /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.broadPeak.gz -b /mnt/data/annotations/by_release/hg20.GRCh38/hg38.blacklist.bed.gz | grep -P 'chr[\dXY]+[ \t]' | awk 'BEGIN{OFS="\t"} {if ($5>1000) $5=1000; print $0}' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.broadPeak.gz
# SYS command. line 74
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.PooledInRep1AndRep2.broadPeak.gz
# SYS command. line 76
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
28283 (process ID) old priority 0, new priority 10
Waiting for 47 seconds.
--------------------Stderr--------------------
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
KI270772.1 80077 80302 Peak_43315 5 . 2.15378 2.49792 0.51405
***** WARNING: File /dev/fd/62 has inconsistent naming convention for record:
KI270772.1 80077 80302 Peak_43315 5 . 2.15378 2.49792 0.51405
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
KI270830.1 14303 17326 Peak_96230 2 . 1.60466 1.71608 0.21683
***** WARNING: File /dev/fd/63 has inconsistent naming convention for record:
KI270830.1 14303 17326 Peak_96230 2 . 1.60466 1.71608 0.21683
|
Num | 22 | ID | task.callpeak_blacklist_filter.blacklist_filter_peak_1.line_27.id_31 | Name | blacklist_filter peak 1 | Thread | thread_Root | PID | 33850 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:16:56 | End | 2017-01-27 17:18:09 | Elapsed | 00:01:12 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.filt.narrowPeak.gz
| Dependencies | | |
# SYS command. line 29
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 31
bedtools intersect -v -a /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz -b /mnt/data/annotations/by_release/hg20.GRCh38/hg38.blacklist.bed.gz \
| awk 'BEGIN{OFS="\t"} {if ($5>1000) $5=1000; print $0}' \
| grep -P 'chr[\dXY]+[ \t]' | gzip -nc > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.filt.narrowPeak.gz
# SYS command. line 35
TASKTIME=$[$(date +%s)-${STARTTIME}]; if [ ${TASKTIME} -lt 60 ]; then echo "Waiting for $[60-${TASKTIME}] seconds."; sleep $[60-${TASKTIME}]; fi
--------------------Stdout--------------------
33854 (process ID) old priority 0, new priority 10
Waiting for 60 seconds.
--------------------Stderr--------------------
***** WARNING: File /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz has inconsistent naming convention for record:
GL383557.1 54713 54863 Peak_23839 40 . 4.14698 4.08901 1.34252 47
***** WARNING: File /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/rep1/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.narrowPeak.gz has inconsistent naming convention for record:
GL383557.1 54713 54863 Peak_23839 40 . 4.14698 4.08901 1.34252 47
|
Num | 23 | ID | task.report.peak2hammock.line_412.id_32 | Name | peak2hammock | Thread | thread_Root | PID | 39343 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:18:10 | End | 2017-01-27 17:18:26 | Elapsed | 00:00:16 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.hammock.gz
| Dependencies | | |
# SYS command. line 414
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 415
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.gz | sed '/^\(chr\)/!d' | sort -k1,1V -k2,2n > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.tmp
# SYS command. line 417
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils/narrowpeak.py /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.tmp /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.hammock
# SYS command. line 418
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.narrowPeak.tmp
--------------------Stdout--------------------
39349 (process ID) old priority 0, new priority 10
|
Num | 24 | ID | task.report.peak2hammock.line_412.id_33 | Name | peak2hammock | Thread | thread_Root | PID | 40877 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:18:27 | End | 2017-01-27 17:18:47 | Elapsed | 00:00:20 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.gz
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.hammock.gz
| Dependencies | | |
# SYS command. line 414
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 415
zcat /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.gz | sed '/^\(chr\)/!d' | sort -k1,1V -k2,2n > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.tmp
# SYS command. line 417
/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils/gappedpeak.py /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.tmp /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.hammock
# SYS command. line 418
rm -f /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/peak/macs2/overlap/SRR2532030.nodup.tagAlign_x_SRR2532031.nodup.tagAlign.naive_overlap.filt.gappedPeak.tmp
--------------------Stdout--------------------
40881 (process ID) old priority 0, new priority 10
|
Num | 25 | ID | task.graphviz.report.line_97.id_34 | Name | report | Thread | thread_Root | PID | 42956 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:18:48 | End | 2017-01-27 17:19:05 | Elapsed | 00:00:17 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | | Output files | | Dependencies | | |
# SYS command. line 98
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 99
dot -Tsvg /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/report/H3K9ac_workflow.dot > /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/report/H3K9ac_workflow.svg 2> /dev/null || echo "svg: /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/report/H3K9ac_workflow.svg" # to suppress dot font error (exit code=1)
--------------------Stdout--------------------
42961 (process ID) old priority 0, new priority 10
|
Num | 26 | ID | task.report.pdf2png.line_379.id_39 | Name | pdf2png | Thread | thread_Root | PID | 42962 | | OK | true | Exit Code | 0 | Retries | | State | FINISHED | Dep. | OK | Cpus | | Mem | | | Start | 2017-01-27 17:18:48 | End | 2017-01-27 17:19:04 | Elapsed | 00:00:15 | Timeout | 00:00:-1 | Wall Timeout | 100 days | | Input files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.pdf
| Output files | /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.png
| Dependencies | | |
# SYS command. line 380
if [[ -f $(which conda) && $(conda env list | grep aquas_chipseq | wc -l) != "0" ]]; then source activate aquas_chipseq; sleep 5; fi; export PATH=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/.:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/modules:/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/src/chipseq_pipeline/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s); renice -n 10 $$
# SYS command. line 381
gs -dFirstPage=1 -dLastPage=1 -dTextAlphaBits=4 -dGraphicsAlphaBits=4 -r110x110 \
-dUseCropBox -dQUIET -dSAFER -dBATCH -dNOPAUSE -dNOPROMPT -sDEVICE=png16m \
-sOutputFile=/srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.png \
-r144 /srv/scratch/shared/surya/imk1/TFBindingPredictionProject/Luo2015Data/H3K9ac/out/qc/rep1/SRR2532030.nodup.15M.cc.plot.pdf
--------------------Stdout--------------------
42970 (process ID) old priority 0, new priority 10
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