Pipeline version (latest git commit SHA1):c98662939a310709da28f62bacfb896089a35342 (Tue Jan 10 09:44:38 2017)

Directories and files
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FilesPath
Alignment
Replicate 1
Filtered & deduped bam
Tag-align ./align/rep1/ZBTB12_Rep2.nodup.tagAlign.gz
Control 1
Tag-align
Pseudo-replicates
Replicate 1
Pseudo-replicate 1
Tag-align ./align/pseudo_reps/rep1/pr1/ZBTB12_Rep2.nodup.pr1.tagAlign.gz
Pseudo-replicate 2
Tag-align ./align/pseudo_reps/rep1/pr2/ZBTB12_Rep2.nodup.pr2.tagAlign.gz
Signal tracks
MACS2
Replicate 1
P-value ./signal/macs2/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.pval.signal.bw
Fold enrichment ./signal/macs2/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.fc.signal.bw
Peaks
SPP
Replicate 1
Region peak ./peak/spp/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.regionPeak.gz
Pseudo-replicates
Replicate 1
Pseudo-replicate 1
Region peak ./peak/spp/pseudo_reps/rep1/pr1/ZBTB12_Rep2.nodup.pr1.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.regionPeak.gz
Pseudo-replicate 2
Region peak ./peak/spp/pseudo_reps/rep1/pr2/ZBTB12_Rep2.nodup.pr2.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.regionPeak.gz
Naive overlap
Peak ./peak/spp/overlap/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.naive_overlap.filt.regionPeak.gz
MACS2
Replicate 1
Narrow peak ./peak/macs2/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.narrowPeak.gz
Broad peak ./peak/macs2/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.broadPeak.gz
Gapped peak ./peak/macs2/rep1/ZBTB12_Rep2.nodup.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.gappedPeak.gz
Pseudo-replicates
Replicate 1
Pseudo-replicate 1
Narrow peak ./peak/macs2/pseudo_reps/rep1/pr1/ZBTB12_Rep2.nodup.pr1.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.narrowPeak.gz
Broad peak ./peak/macs2/pseudo_reps/rep1/pr1/ZBTB12_Rep2.nodup.pr1.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.broadPeak.gz
Gapped peak ./peak/macs2/pseudo_reps/rep1/pr1/ZBTB12_Rep2.nodup.pr1.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.gappedPeak.gz
Pseudo-replicate 2
Narrow peak ./peak/macs2/pseudo_reps/rep1/pr2/ZBTB12_Rep2.nodup.pr2.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.narrowPeak.gz
Broad peak ./peak/macs2/pseudo_reps/rep1/pr2/ZBTB12_Rep2.nodup.pr2.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.broadPeak.gz
Gapped peak ./peak/macs2/pseudo_reps/rep1/pr2/ZBTB12_Rep2.nodup.pr2.tagAlign_x_input15MReadsNSCLess1.05.50MSubsample.tagAlign.gappedPeak.gz
IDR
Pseudo-replicates
Replicate 1
IDR peak ./peak/idr/pseudo_reps/rep1/ZBTB12_rep1-pr.IDR0.05.filt.narrowPeak.gz
QC and logs
Replicate 1
Cross-corr. log ./qc/rep1/ZBTB12_Rep2.nodup.15M.cc.qc
Cross-corr. plot ./qc/rep1/ZBTB12_Rep2.nodup.15M.cc.plot.pdf
IDR
IDR QC log ./qc/ZBTB12_IDR_final.qc
Pseudo-replicates
Replicate 1
IDR plot ./peak/idr/pseudo_reps/rep1/ZBTB12_rep1-pr.unthresholded-peaks.txt.png

Visualization
Visualize  JSON (datahub)

Workflow diagram (g. peak : gapped peak from MACS2, n. peak : narrow peak from MACS2, r. peak : region peak from SPP)


Cross-correlation QC (all)
 numReadsestFragLencorr_estFragLenPhantomPeakcorr_phantomPeakargmin_corrmin_corrNSCRSC
rep1
148347231550.182180504724746500.174480815000.17194241.0595444.033277

Normalized strand cross-correlation coefficient (NSC) = col9 in outFile
Relative strand cross-correlation coefficient (RSC) = col10 in outFile
Estimated fragment length = col3 in outFile, take the top value
Important columns highlighted, but all/whole file can be stored for display


rep1
 

IDR QC (idr)
ZBTB12_IDR_final.qc
NtN1N2Npconservative_setoptimal_setrescue_ratioself_consistency_ratioreproducibility
03700N/AN/ANaN1.01

rep1 pseudo-reps