cellbender:remove-background: Command: cellbender remove-background --input /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/inputs/2765-Schizophrenia-dACC.raw_feature_bc_matrix.h5 --output /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output.h5 --cuda --expected-cells 4078 --total-droplets-included 20000 --fpr 0.01 --epochs 150 cellbender:remove-background: CellBender 0.3.0 cellbender:remove-background: (Workflow hash 6f49099fd0) cellbender:remove-background: 2024-06-06 15:33:00 cellbender:remove-background: Running remove-background cellbender:remove-background: Loading data from /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/inputs/2765-Schizophrenia-dACC.raw_feature_bc_matrix.h5 cellbender:remove-background: CellRanger v3 format cellbender:remove-background: Features in dataset: 36601 Gene Expression cellbender:remove-background: Trimming features for inference. cellbender:remove-background: 28732 features have nonzero counts. cellbender:remove-background: Prior on counts for cells is 1035 cellbender:remove-background: Prior on counts for empty droplets is 33 cellbender:remove-background: Excluding 9345 features that are estimated to have <= 0.1 background counts in cells. cellbender:remove-background: Including 19387 features in the analysis. cellbender:remove-background: Trimming barcodes for inference. cellbender:remove-background: Excluding barcodes with counts below 16 cellbender:remove-background: Using 4078 probable cell barcodes, plus an additional 15922 barcodes, and 49760 empty droplets. cellbender:remove-background: Largest surely-empty droplet has 33 UMI counts. cellbender:remove-background: Attempting to unpack tarball "ckpt.tar.gz" to /tmp/tmpobepy6sf cellbender:remove-background: No saved checkpoint. cellbender:remove-background: No checkpoint loaded. cellbender:remove-background: Running inference... cellbender:remove-background: [epoch 001] average training loss: 1246.3848 cellbender:remove-background: [epoch 002] average training loss: 1139.9481 (12.6 seconds per epoch) cellbender:remove-background: Will checkpoint every 34 epochs cellbender:remove-background: [epoch 003] average training loss: 1065.6239 cellbender:remove-background: [epoch 004] average training loss: 1001.0014 cellbender:remove-background: [epoch 005] average training loss: 970.1755 cellbender:remove-background: [epoch 005] average test loss: 956.0362 cellbender:remove-background: [epoch 006] average training loss: 956.7989 cellbender:remove-background: [epoch 007] average training loss: 950.3773 cellbender:remove-background: [epoch 008] average training loss: 949.1921 cellbender:remove-background: [epoch 009] average training loss: 937.3276 cellbender:remove-background: [epoch 010] average training loss: 935.2232 cellbender:remove-background: [epoch 010] average test loss: 932.6829 cellbender:remove-background: [epoch 011] average training loss: 934.6154 cellbender:remove-background: [epoch 012] average training loss: 939.5972 cellbender:remove-background: [epoch 013] average training loss: 926.0896 cellbender:remove-background: [epoch 014] average training loss: 897.5801 cellbender:remove-background: [epoch 015] average training loss: 886.0498 cellbender:remove-background: [epoch 015] average test loss: 874.5343 cellbender:remove-background: [epoch 016] average training loss: 883.8048 cellbender:remove-background: [epoch 017] average training loss: 884.2185 cellbender:remove-background: [epoch 018] average training loss: 884.4592 cellbender:remove-background: [epoch 019] average training loss: 883.8393 cellbender:remove-background: [epoch 020] average training loss: 882.2867 cellbender:remove-background: [epoch 020] average test loss: 873.4734 cellbender:remove-background: [epoch 021] average training loss: 880.8295 cellbender:remove-background: [epoch 022] average training loss: 881.2554 cellbender:remove-background: [epoch 023] average training loss: 880.2017 cellbender:remove-background: [epoch 024] average training loss: 879.5333 cellbender:remove-background: [epoch 025] average training loss: 878.6403 cellbender:remove-background: [epoch 025] average test loss: 869.0924 cellbender:remove-background: [epoch 026] average training loss: 873.9961 cellbender:remove-background: [epoch 027] average training loss: 874.7936 cellbender:remove-background: [epoch 028] average training loss: 872.8871 cellbender:remove-background: [epoch 029] average training loss: 870.8695 cellbender:remove-background: [epoch 030] average training loss: 868.2787 cellbender:remove-background: [epoch 030] average test loss: 860.4262 cellbender:remove-background: [epoch 031] average training loss: 871.8099 cellbender:remove-background: [epoch 032] average training loss: 871.4959 cellbender:remove-background: [epoch 033] average training loss: 869.6184 cellbender:remove-background: [epoch 034] average training loss: 869.7730 cellbender:remove-background: Saving a checkpoint... cellbender:remove-background: Saved checkpoint as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/ckpt.tar.gz cellbender:remove-background: [epoch 035] average training loss: 871.9921 cellbender:remove-background: [epoch 035] average test loss: 855.3105 cellbender:remove-background: [epoch 036] average training loss: 866.2534 cellbender:remove-background: [epoch 037] average training loss: 865.7746 cellbender:remove-background: [epoch 038] average training loss: 868.3951 cellbender:remove-background: [epoch 039] average training loss: 865.0425 cellbender:remove-background: [epoch 040] average training loss: 868.9556 cellbender:remove-background: [epoch 040] average test loss: 873.6281 cellbender:remove-background: [epoch 041] average training loss: 864.2196 cellbender:remove-background: [epoch 042] average training loss: 868.2923 cellbender:remove-background: [epoch 043] average training loss: 862.2534 cellbender:remove-background: [epoch 044] average training loss: 864.2241 cellbender:remove-background: [epoch 045] average training loss: 864.7072 cellbender:remove-background: [epoch 045] average test loss: 855.5674 cellbender:remove-background: [epoch 046] average training loss: 860.8445 cellbender:remove-background: [epoch 047] average training loss: 860.4847 cellbender:remove-background: [epoch 048] average training loss: 857.7808 cellbender:remove-background: [epoch 049] average training loss: 861.9181 cellbender:remove-background: [epoch 050] average training loss: 867.2270 cellbender:remove-background: [epoch 050] average test loss: 859.9057 cellbender:remove-background: [epoch 051] average training loss: 864.8651 cellbender:remove-background: [epoch 052] average training loss: 859.9632 cellbender:remove-background: [epoch 053] average training loss: 864.0469 cellbender:remove-background: [epoch 054] average training loss: 862.2330 cellbender:remove-background: [epoch 055] average training loss: 862.2210 cellbender:remove-background: [epoch 055] average test loss: 855.5473 cellbender:remove-background: [epoch 056] average training loss: 862.2981 cellbender:remove-background: [epoch 057] average training loss: 863.8105 cellbender:remove-background: [epoch 058] average training loss: 859.9475 cellbender:remove-background: [epoch 059] average training loss: 863.0809 cellbender:remove-background: [epoch 060] average training loss: 860.8894 cellbender:remove-background: [epoch 060] average test loss: 856.1591 cellbender:remove-background: [epoch 061] average training loss: 863.7391 cellbender:remove-background: [epoch 062] average training loss: 858.5907 cellbender:remove-background: [epoch 063] average training loss: 860.9249 cellbender:remove-background: [epoch 064] average training loss: 860.3529 cellbender:remove-background: [epoch 065] average training loss: 858.4182 cellbender:remove-background: [epoch 065] average test loss: 858.1175 cellbender:remove-background: [epoch 066] average training loss: 862.1903 cellbender:remove-background: [epoch 067] average training loss: 859.6324 cellbender:remove-background: [epoch 068] average training loss: 858.3241 cellbender:remove-background: Saving a checkpoint... cellbender:remove-background: Saved checkpoint as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/ckpt.tar.gz cellbender:remove-background: [epoch 069] average training loss: 857.5372 cellbender:remove-background: [epoch 070] average training loss: 859.5930 cellbender:remove-background: [epoch 070] average test loss: 861.0841 cellbender:remove-background: [epoch 071] average training loss: 859.6300 cellbender:remove-background: [epoch 072] average training loss: 858.6728 cellbender:remove-background: [epoch 073] average training loss: 859.4769 cellbender:remove-background: [epoch 074] average training loss: 857.9346 cellbender:remove-background: [epoch 075] average training loss: 858.1020 cellbender:remove-background: [epoch 075] average test loss: 857.2782 cellbender:remove-background: [epoch 076] average training loss: 857.9021 cellbender:remove-background: [epoch 077] average training loss: 857.1262 cellbender:remove-background: [epoch 078] average training loss: 856.4386 cellbender:remove-background: [epoch 079] average training loss: 855.6383 cellbender:remove-background: [epoch 080] average training loss: 854.9047 cellbender:remove-background: [epoch 080] average test loss: 887.9949 cellbender:remove-background: [epoch 081] average training loss: 855.2482 cellbender:remove-background: [epoch 082] average training loss: 854.3704 cellbender:remove-background: [epoch 083] average training loss: 855.6282 cellbender:remove-background: [epoch 084] average training loss: 857.4137 cellbender:remove-background: [epoch 085] average training loss: 854.2380 cellbender:remove-background: [epoch 085] average test loss: 868.9844 cellbender:remove-background: [epoch 086] average training loss: 856.6327 cellbender:remove-background: [epoch 087] average training loss: 855.4098 cellbender:remove-background: [epoch 088] average training loss: 854.1725 cellbender:remove-background: [epoch 089] average training loss: 856.9719 cellbender:remove-background: [epoch 090] average training loss: 855.2912 cellbender:remove-background: [epoch 090] average test loss: 865.6894 cellbender:remove-background: [epoch 091] average training loss: 858.8681 cellbender:remove-background: [epoch 092] average training loss: 855.8336 cellbender:remove-background: [epoch 093] average training loss: 858.2648 cellbender:remove-background: [epoch 094] average training loss: 862.6940 cellbender:remove-background: [epoch 095] average training loss: 857.8862 cellbender:remove-background: [epoch 095] average test loss: 873.7659 cellbender:remove-background: [epoch 096] average training loss: 857.7173 cellbender:remove-background: [epoch 097] average training loss: 856.9769 cellbender:remove-background: [epoch 098] average training loss: 859.9789 cellbender:remove-background: [epoch 099] average training loss: 856.7475 cellbender:remove-background: [epoch 100] average training loss: 859.1232 cellbender:remove-background: [epoch 100] average test loss: 869.6779 cellbender:remove-background: [epoch 101] average training loss: 862.4822 cellbender:remove-background: [epoch 102] average training loss: 857.0137 cellbender:remove-background: Saving a checkpoint... cellbender:remove-background: Saved checkpoint as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/ckpt.tar.gz cellbender:remove-background: [epoch 103] average training loss: 859.2105 cellbender:remove-background: [epoch 104] average training loss: 857.4943 cellbender:remove-background: [epoch 105] average training loss: 859.4585 cellbender:remove-background: [epoch 105] average test loss: 864.4205 cellbender:remove-background: [epoch 106] average training loss: 860.7730 cellbender:remove-background: [epoch 107] average training loss: 859.8728 cellbender:remove-background: [epoch 108] average training loss: 857.3123 cellbender:remove-background: [epoch 109] average training loss: 859.0283 cellbender:remove-background: [epoch 110] average training loss: 856.9692 cellbender:remove-background: [epoch 110] average test loss: 874.6150 cellbender:remove-background: [epoch 111] average training loss: 855.8490 cellbender:remove-background: [epoch 112] average training loss: 856.4322 cellbender:remove-background: [epoch 113] average training loss: 856.0946 cellbender:remove-background: [epoch 114] average training loss: 857.0701 cellbender:remove-background: [epoch 115] average training loss: 856.9363 cellbender:remove-background: [epoch 115] average test loss: 869.9483 cellbender:remove-background: [epoch 116] average training loss: 861.4908 cellbender:remove-background: [epoch 117] average training loss: 858.8182 cellbender:remove-background: [epoch 118] average training loss: 856.6074 cellbender:remove-background: [epoch 119] average training loss: 857.9278 cellbender:remove-background: [epoch 120] average training loss: 858.0930 cellbender:remove-background: [epoch 120] average test loss: 871.1397 cellbender:remove-background: [epoch 121] average training loss: 856.7892 cellbender:remove-background: [epoch 122] average training loss: 858.5744 cellbender:remove-background: [epoch 123] average training loss: 859.5566 cellbender:remove-background: [epoch 124] average training loss: 859.2904 cellbender:remove-background: [epoch 125] average training loss: 858.0783 cellbender:remove-background: [epoch 125] average test loss: 871.8281 cellbender:remove-background: [epoch 126] average training loss: 857.8839 cellbender:remove-background: [epoch 127] average training loss: 857.0891 cellbender:remove-background: [epoch 128] average training loss: 858.9661 cellbender:remove-background: [epoch 129] average training loss: 856.6812 cellbender:remove-background: [epoch 130] average training loss: 856.8326 cellbender:remove-background: [epoch 130] average test loss: 873.5326 cellbender:remove-background: [epoch 131] average training loss: 859.9279 cellbender:remove-background: [epoch 132] average training loss: 858.9683 cellbender:remove-background: [epoch 133] average training loss: 859.0133 cellbender:remove-background: [epoch 134] average training loss: 859.2419 cellbender:remove-background: [epoch 135] average training loss: 860.8646 cellbender:remove-background: [epoch 135] average test loss: 879.5207 cellbender:remove-background: [epoch 136] average training loss: 857.7292 cellbender:remove-background: Saving a checkpoint... cellbender:remove-background: Saved checkpoint as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/ckpt.tar.gz cellbender:remove-background: [epoch 137] average training loss: 859.7368 cellbender:remove-background: [epoch 138] average training loss: 859.4686 cellbender:remove-background: [epoch 139] average training loss: 857.4154 cellbender:remove-background: [epoch 140] average training loss: 858.7943 cellbender:remove-background: [epoch 140] average test loss: 874.1697 cellbender:remove-background: [epoch 141] average training loss: 857.8994 cellbender:remove-background: [epoch 142] average training loss: 860.7788 cellbender:remove-background: [epoch 143] average training loss: 857.3106 cellbender:remove-background: [epoch 144] average training loss: 858.4869 cellbender:remove-background: [epoch 145] average training loss: 858.0502 cellbender:remove-background: [epoch 145] average test loss: 876.1545 cellbender:remove-background: [epoch 146] average training loss: 859.7550 cellbender:remove-background: [epoch 147] average training loss: 857.0246 cellbender:remove-background: [epoch 148] average training loss: 858.4127 cellbender:remove-background: [epoch 149] average training loss: 858.4306 cellbender:remove-background: [epoch 150] average training loss: 858.1166 cellbender:remove-background: [epoch 150] average test loss: 882.5369 cellbender:remove-background: Saving a checkpoint... cellbender:remove-background: Saved checkpoint as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/ckpt.tar.gz cellbender:remove-background: 2024-06-06 16:06:45 cellbender:remove-background: Inference procedure complete. cellbender:remove-background: Attempting to unpack tarball "ckpt.tar.gz" to /tmp/tmp79pdo16w cellbender:remove-background: Successfully unpacked tarball to /tmp/tmp79pdo16w /tmp/tmp79pdo16w/6f49099fd0_random.pyro /tmp/tmp79pdo16w/6f49099fd0_random.cuda /tmp/tmp79pdo16w/6f49099fd0_model.torch /tmp/tmp79pdo16w/6f49099fd0_optim.torch /tmp/tmp79pdo16w/6f49099fd0_optim.pyro /tmp/tmp79pdo16w/6f49099fd0_params.pyro /tmp/tmp79pdo16w/6f49099fd0_train.loaderstate /tmp/tmp79pdo16w/6f49099fd0_test.loaderstate /tmp/tmp79pdo16w/6f49099fd0_args.npy cellbender:remove-background: Posterior not currently included in checkpoint. cellbender:remove-background: Computing posterior noise count probabilities in mini-batches. cellbender:remove-background: Working on chunk (1/32) cellbender:remove-background: [0.05 mins per chunk] cellbender:remove-background: Working on chunk (2/32) cellbender:remove-background: Working on chunk (3/32) cellbender:remove-background: Working on chunk (4/32) cellbender:remove-background: Working on chunk (5/32) cellbender:remove-background: Working on chunk (6/32) cellbender:remove-background: Working on chunk (7/32) cellbender:remove-background: Working on chunk (8/32) cellbender:remove-background: Working on chunk (9/32) cellbender:remove-background: Working on chunk (10/32) cellbender:remove-background: Working on chunk (11/32) cellbender:remove-background: Working on chunk (12/32) cellbender:remove-background: Working on chunk (13/32) cellbender:remove-background: Working on chunk (14/32) cellbender:remove-background: Working on chunk (15/32) cellbender:remove-background: Working on chunk (16/32) cellbender:remove-background: Working on chunk (17/32) cellbender:remove-background: Working on chunk (18/32) cellbender:remove-background: Working on chunk (19/32) cellbender:remove-background: Working on chunk (20/32) cellbender:remove-background: Working on chunk (21/32) cellbender:remove-background: Working on chunk (22/32) cellbender:remove-background: Working on chunk (23/32) cellbender:remove-background: Working on chunk (24/32) cellbender:remove-background: Working on chunk (25/32) cellbender:remove-background: Working on chunk (26/32) cellbender:remove-background: Working on chunk (27/32) cellbender:remove-background: Working on chunk (28/32) cellbender:remove-background: Working on chunk (29/32) cellbender:remove-background: Working on chunk (30/32) cellbender:remove-background: Working on chunk (31/32) cellbender:remove-background: Working on chunk (32/32) cellbender:remove-background: Writing full posterior to /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_posterior.h5 cellbender:remove-background: Succeeded in writing posterior to file /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_posterior.h5 cellbender:remove-background: Added posterior object to checkpoint file. cellbender:remove-background: 2024-06-06 16:09:51 cellbender:remove-background: Saved summary plots as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output.pdf cellbender:remove-background: Saved cell barcodes in /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_cell_barcodes.csv cellbender:remove-background: Computing target noise counts per gene for MCKP estimator cellbender:remove-background: Using MCKP noise targets computed for FPR 0.01 cellbender:remove-background: Computing denoised counts using mckp estimator cellbender:remove-background: Working on chunk (1/7) cellbender:remove-background: [0.39 mins per chunk] cellbender:remove-background: Working on chunk (2/7) cellbender:remove-background: Working on chunk (3/7) cellbender:remove-background: Working on chunk (4/7) cellbender:remove-background: Working on chunk (5/7) cellbender:remove-background: Working on chunk (6/7) cellbender:remove-background: Working on chunk (7/7) cellbender:remove-background: 2024-06-06 16:12:46 Total MCKP estimation time = 164.30 sec cellbender:remove-background: Succeeded in writing CellRanger format output to file /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output.h5 cellbender:remove-background: Succeeded in writing CellRanger format output to file /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_filtered.h5 cellbender:remove-background: Saved output metrics as /oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_metrics.csv Traceback (most recent call last): File "/home/users/guozy/miniconda3/envs/cellbender/bin/jupyter-nbconvert", line 5, in from nbconvert.nbconvertapp import main File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/__init__.py", line 5, in from .exporters import * File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/__init__.py", line 1, in from .asciidoc import ASCIIDocExporter File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/asciidoc.py", line 9, in from .templateexporter import TemplateExporter File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/templateexporter.py", line 25, in from lxml.html.clean import clean_html File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/lxml/html/clean.py", line 21, in ) from None ImportError: lxml.html.clean module is now a separate project lxml_html_clean. Install lxml[html_clean] or lxml_html_clean directly. Traceback (most recent call last): File "/home/users/guozy/miniconda3/envs/cellbender/bin/jupyter-nbconvert", line 5, in from nbconvert.nbconvertapp import main File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/__init__.py", line 5, in from .exporters import * File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/__init__.py", line 1, in from .asciidoc import ASCIIDocExporter File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/asciidoc.py", line 9, in from .templateexporter import TemplateExporter File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/nbconvert/exporters/templateexporter.py", line 25, in from lxml.html.clean import clean_html File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/lxml/html/clean.py", line 21, in ) from None ImportError: lxml.html.clean module is now a separate project lxml_html_clean. Install lxml[html_clean] or lxml_html_clean directly. mv: cannot stat ‘tmp.report.nbconvert.html’: No such file or directory rm: cannot remove ‘tmp.report.nbconvert.ipynb’: No such file or directory cellbender:remove-background: Unable to create report. cellbender:remove-background: Traceback (most recent call last): File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/cellbender/remove_background/run.py", line 351, in compute_output_denoised_counts_reports_metrics output=html_report_file, File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/cellbender/remove_background/report.py", line 82, in run_notebook_make_html title=('CellBender: ' + os.path.basename(output).replace('_report.html', '')), File "/home/users/guozy/miniconda3/envs/cellbender/lib/python3.7/site-packages/cellbender/remove_background/report.py", line 59, in _postprocess_html with open(file, mode='r') as f: FileNotFoundError: [Errno 2] No such file or directory: '/oak/stanford/groups/akundaje/projects/psychencode/outs/20231009_multiome_analysis/01-cellbender/outputs/2765-Schizophrenia-dACC/2765-Schizophrenia-dACC.cellbender.output_report.html' cellbender:remove-background: Completed remove-background. cellbender:remove-background: 2024-06-06 16:12:55