FastQCFastQC Report
Mon 27 Jun 2016
RHE891-ATCACG_S1_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRHE891-ATCACG_S1_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7015807
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA7600.012.7135784
CGTATCG5950.012.1793953
CCGGCAT13700.010.3300221
CGACTAA5805.995389E-910.11312728
CCCGTAT8250.010.0414231
ATCGAAG15800.09.8283086
CGCCAAC15150.09.7970141
ATCGCGC6655.640686E-99.3374667
CGCATAT3350.0013896469.2733291
CCGTATC9750.09.2022742
TTTAGCG3753.809349E-49.1988657
CGCGTAC4151.0321032E-49.1491871
CGAGAAT11450.09.0438581
TATCGAA8455.4569682E-118.98440555
CGACGAT5806.5299355E-78.926911
CGGAAAT13550.08.9159311
GCGCTAA4653.6588248E-58.9034642
CGCCTAT5857.3904266E-78.8506131
ATATCGC4301.5245948E-48.82688236
CTCGGGT14900.08.8030931
Produced by FastQC (version 0.11.4)