##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H7hESC-p7c7r7-trimmed-pair1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3660917	
Filtered Sequences	0	
Sequence length	44-151	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33492073160905	32.0	32.0	32.0	32.0	32.0
2	31.506779585551925	32.0	32.0	32.0	32.0	32.0
3	35.267907466899686	37.0	32.0	37.0	32.0	37.0
4	35.879269592836984	37.0	37.0	37.0	32.0	37.0
5	36.151767985999136	37.0	37.0	37.0	37.0	37.0
6	39.522622064362565	41.0	41.0	41.0	37.0	41.0
7	39.72407869394471	41.0	41.0	41.0	37.0	41.0
8	39.78581896284455	41.0	41.0	41.0	37.0	41.0
9	39.72965489247639	41.0	41.0	41.0	37.0	41.0
10-14	39.75193417386956	41.0	41.0	41.0	37.0	41.0
15-19	39.75479050740566	41.0	41.0	41.0	37.0	41.0
20-24	39.73975143386206	41.0	41.0	41.0	37.0	41.0
25-29	39.63369205037973	41.0	41.0	41.0	37.0	41.0
30-34	39.5409246371879	41.0	41.0	41.0	37.0	41.0
35-39	39.51724696298769	41.0	41.0	41.0	37.0	41.0
40-44	39.44978107943993	41.0	41.0	41.0	37.0	41.0
45-49	39.35956389056728	41.0	41.0	41.0	37.0	41.0
50-54	39.26471081683131	41.0	41.0	41.0	37.0	41.0
55-59	39.19016524557385	41.0	41.0	41.0	37.0	41.0
60-64	39.131117603112884	41.0	41.0	41.0	37.0	41.0
65-69	39.05436384073381	41.0	41.0	41.0	37.0	41.0
70-74	38.93396377681536	41.0	41.0	41.0	33.0	41.0
75-79	38.42209753572536	41.0	39.4	41.0	32.0	41.0
80-84	38.76463884028993	41.0	41.0	41.0	32.0	41.0
85-89	38.71189016953412	41.0	41.0	41.0	32.0	41.0
90-94	38.66154441011149	41.0	41.0	41.0	32.0	41.0
95-99	38.5798633893066	41.0	41.0	41.0	32.0	41.0
100-104	38.4564688385782	41.0	41.0	41.0	32.0	41.0
105-109	38.29389570266831	41.0	41.0	41.0	32.0	41.0
110-114	38.17720843613947	41.0	41.0	41.0	32.0	41.0
115-119	37.949241763365286	41.0	37.0	41.0	32.0	41.0
120-124	37.67954200169861	41.0	37.0	41.0	27.0	41.0
125-129	37.389312035711725	41.0	37.0	41.0	27.0	41.0
130-134	37.04771157927724	41.0	37.0	41.0	26.0	41.0
135-139	36.5862123421651	41.0	37.0	41.0	22.0	41.0
140-144	36.33720935597737	41.0	37.0	41.0	22.0	41.0
145-149	36.14635926894182	41.0	37.0	41.0	22.0	41.0
150-151	36.089050520424436	39.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	4.0
15	11.0
16	12.0
17	56.0
18	202.0
19	626.0
20	1749.0
21	3683.0
22	5212.0
23	8460.0
24	13073.0
25	18327.0
26	24566.0
27	28367.0
28	33763.0
29	41108.0
30	49578.0
31	59241.0
32	69805.0
33	83273.0
34	99438.0
35	118364.0
36	143392.0
37	182068.0
38	256046.0
39	466464.0
40	1954024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.617561141462424	18.278969702129476	12.012467687189824	25.091001469218277
2	16.163620999289467	19.579426975473634	39.59649178923287	24.660460236004024
3	18.997999681500563	24.947629241526098	28.311130790454957	27.74324028651838
4	12.628971682994905	15.419686514209053	37.710703956347515	34.24063784644852
5	13.41480836631915	37.58948919082295	34.8679579460556	14.127744496802306
6	32.369418146287956	36.72080358315967	17.147982342061507	13.761795928490866
7	28.776123577781192	31.23526154785809	21.770419815581725	18.21819505877899
8	26.29735432045348	35.15522907952991	20.05486610986552	18.49255049015109
9	26.157424100382205	15.68955400984679	20.088951096018235	38.06407079375278
10-14	24.652858567403737	23.66188751432634	27.98776327508577	23.697490643184153
15-19	22.73532064429604	27.776477859323307	26.196888459584976	23.291313036795678
20-24	23.070699327430365	26.36793548803351	27.704246365102314	22.85711881943381
25-29	22.72735020452903	26.475211658427888	27.799640656512075	22.99779748053101
30-34	22.737140910701296	26.615265728880903	27.8130062801617	22.834587080256103
35-39	22.81395324824774	26.64906040158827	27.694209539757754	22.842776810406246
40-44	22.515016617873265	26.43660634816089	27.98557373237499	23.062803301590854
45-49	22.601641659675135	26.430314307347007	27.94599798812092	23.02204604485694
50-54	22.554202947513616	26.492931254602397	27.86511088727796	23.087754910606023
55-59	22.483622324042656	26.485198562504614	27.908598929313854	23.122580184138876
60-64	22.57293766010556	26.685478244765036	27.628102203139914	23.11348189198949
65-69	22.451139812448208	26.672000272726322	27.599777841685867	23.277082073139603
70-74	22.523447589146294	26.57318771718597	27.576169904590326	23.327194789077414
75-79	22.572812245041582	26.664702947283807	27.409752505791168	23.352732301883446
80-84	22.483383526595432	26.634178255067486	27.493504176284944	23.38893404205214
85-89	22.53763735458792	26.57226643215021	27.387707827598916	23.50238838566295
90-94	22.421006341324855	26.520573670075294	27.4091852315157	23.649234757084155
95-99	22.302724988280147	26.474850949844924	27.447261476729455	23.77516258514547
100-104	22.268358834123173	26.59996115674238	27.399154353326683	23.732525655807763
105-109	22.179895937361373	26.603213660570486	27.339098444305073	23.87779195776307
110-114	22.036196294182425	26.888109842260267	27.238129037372367	23.83756482618494
115-119	22.0671202157464	26.958531601938212	27.138764613337905	23.835583568977476
120-124	21.90448201956541	26.985520686485803	27.169043735699056	23.940953558249735
125-129	21.798523240181435	27.18403785380021	27.0233320882384	23.994106817779954
130-134	21.843585368101472	27.156437699291065	26.916979579672752	24.08299735293471
135-139	21.56152457042793	27.32450476086243	26.88034912991135	24.233621538798293
140-144	21.536438840251147	27.50544981445343	26.708530628322407	24.249580716973014
145-149	21.520673369755425	27.464180069815264	26.602019890738188	24.413126669691128
150-151	21.58183543684034	27.671771152592495	26.25239582599295	24.49399758457422
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	69.7085626106807
2	40.82408123529349
3	46.741239968018945
4	46.86960952944343
5	27.542552863121454
6	46.13121407477882
7	46.99431863656018
8	44.78990481060457
9	64.22149489413498
10-14	48.350349210587886
15-19	46.02663368109172
20-24	45.927818146864176
25-29	45.725147685060044
30-34	45.5717279909574
35-39	45.656730058653984
40-44	45.57781991946412
45-49	45.623687704532074
50-54	45.64195785811964
55-59	45.60620250818153
60-64	45.68641955209505
65-69	45.728221885587814
70-74	45.850642378223704
75-79	45.925544546925025
80-84	45.87231756864757
85-89	46.04002574025087
90-94	46.07024109840901
95-99	46.07788757342561
100-104	46.00088448993094
105-109	46.05768789512444
110-114	45.87376112036737
115-119	45.90270378472388
120-124	45.845435577815145
125-129	45.79263005796139
130-134	45.92658272103618
135-139	45.79514610922622
140-144	45.78601955722416
145-149	45.933800039446545
150-151	46.07583302141456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	83.5
2	520.5
3	1019.0
4	932.0
5	807.0
6	1395.5
7	3188.5
8	5790.0
9	6512.5
10	4942.0
11	3367.5
12	2522.0
13	2127.0
14	1801.0
15	1581.5
16	1450.0
17	1338.5
18	1339.0
19	1348.5
20	1394.0
21	1568.5
22	1880.5
23	2570.5
24	3632.0
25	5022.5
26	6988.5
27	9762.0
28	13941.0
29	19591.5
30	26664.0
31	35167.5
32	44858.0
33	56396.5
34	68449.0
35	80355.0
36	92053.5
37	102739.0
38	113893.5
39	122691.5
40	129022.0
41	135789.5
42	140805.5
43	145282.0
44	145891.5
45	144417.5
46	143882.0
47	141841.0
48	138948.5
49	136431.5
50	135235.5
51	130661.0
52	120753.0
53	112362.5
54	106947.0
55	101643.5
56	94322.5
57	85283.5
58	77907.5
59	70934.0
60	63672.0
61	56349.5
62	49124.5
63	42021.5
64	34544.5
65	27958.0
66	22933.5
67	18115.5
68	14017.0
69	11071.0
70	8597.0
71	6651.0
72	4931.5
73	3443.5
74	2606.0
75	1803.5
76	1121.5
77	755.5
78	523.5
79	300.5
80	157.0
81	85.5
82	35.0
83	12.5
84	6.5
85	2.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21726796865375533
2	0.08462360659911164
3	0.0
4	0.030101747731510985
5	0.0
6	3.824178477687421E-4
7	0.0
8	1.3657780277455075E-4
9	7.921512560923944E-4
10-14	7.648356955374842E-5
15-19	9.833601799767654E-5
20-24	6.555734533178436E-5
25-29	5.080694263213287E-4
30-34	6.555734533178436E-4
35-39	6.337210048739155E-4
40-44	0.0010762330858634598
45-49	0.0016334709673753783
50-54	0.0014531886154144523
55-59	0.0013002213927392468
60-64	0.0013384631984080483
65-69	0.0027916518138225007
70-74	0.0030648075685996536
75-79	0.014045668910641192
80-84	0.06164032762301228
85-89	0.06035103246046412
90-94	0.007102050400192346
95-99	8.795617706397911E-4
100-104	0.0012729063040438867
105-109	0.0010270671529283323
110-114	0.0018301536553303852
115-119	0.0010762510188873859
120-124	0.0013166762676587594
125-129	0.0011037437128954967
130-134	0.0017710196251932134
135-139	0.0024296224826320296
140-144	0.002449109723904612
145-149	9.588385120833163E-4
150-151	9.609141313404888E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	1.0
45-49	1.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	1.0
95-99	0.0
100-104	3.0
105-109	9.0
110-114	27.0
115-119	68.0
120-124	268.0
125-129	861.0
130-134	2462.0
135-139	7570.0
140-144	25372.0
145-149	176570.0
150-152	3447704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	60.65564463476847
#Duplication Level	Relative count
1	100.0
2	39.85900105130795
3	18.63288738636598
4	10.189441569953207
5	6.488219166786914
6	4.327884397353178
7	3.179691204056812
8	2.5447836573148357
9	2.1706417101276
10++	18.746263733998475
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11903	0.32513711728509553	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6054	0.16536840359942603	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5524	0.15089115650532364	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	3799	0.10377181454810365	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	5731960	7.055414	11.38118	25-29
AAAAA	3368495	4.664425	11.892793	145-147
CAGGC	1213220	3.0190477	5.46257	145-147
GGCAG	1138885	2.9665046	5.3221893	145-147
CTGTC	1359250	2.7240386	5.323158	145-147
GCCCA	1138635	2.70695	5.3052673	1
GAGCC	1081420	2.691069	5.797527	145-147
GTCTC	1293010	2.5912886	5.3124957	145-147
GCAGA	1155455	2.5407317	10.569439	9
AGCCC	1064800	2.5314174	5.383128	145-147
ACAGG	1114905	2.451566	5.382241	8
ATCTC	1448610	2.450789	5.918172	145-147
ACACA	1374835	2.43816	6.849892	6
CTGGG	940840	2.3936043	7.9563966	4
GCTGG	935105	2.3790138	5.2607284	3
GGAGG	871475	2.376046	5.1385775	8
CAGAA	1276640	2.3698165	5.0430737	4
CCAGG	934380	2.3251662	9.891681	3
GGCTG	907160	2.3079185	6.7867146	7
CCTGG	928775	2.25742	8.447738	3
CCCAG	942700	2.2411413	7.793834	2
CTGCT	1099110	2.2026985	5.4210224	9
AGGAG	951625	2.1903138	6.676524	5
GACAG	983890	2.163477	6.581518	7
TGCTG	1029810	2.1602583	5.8055673	2
CAGCA	999005	2.0986433	6.155414	4
GCCTG	853870	2.0753608	5.003109	7
AGAAG	1036750	2.0144434	5.1590934	5
TCCTG	1001835	2.0077522	8.551227	2
AGACA	1076360	1.9980388	5.0131407	6
CAGGA	899980	1.9789672	8.800352	4
AGCAG	895030	1.9680825	5.11466	7
CAGAG	894240	1.9663454	10.6513605	4
CTCCT	1019925	1.9527534	6.3004446	1
TGGGG	714605	1.9029938	10.002864	6
TGGAG	844935	1.899482	6.3629074	5
GGCCA	739845	1.8410738	5.3317156	1
CTGGA	853630	1.8333561	7.4663424	4
GGGAG	672300	1.8330023	5.6222553	7
TACAC	1042245	1.8053143	6.72981	5
GGAGA	780975	1.797536	5.0432477	6
CAGGG	681110	1.7741176	8.329368	4
TGCAG	821435	1.7642103	6.1046977	2
CCCTG	759390	1.7633237	5.846923	2
TCCAG	851990	1.748143	7.7686844	2
CACAG	825220	1.7335674	6.643426	7
CTCTG	847990	1.6994354	5.099261	2
CTGTG	805910	1.6905776	8.236261	9
CAGTG	784560	1.6850133	6.8565593	9
AGAGG	724705	1.6680217	5.088804	5
CACTG	810345	1.6626943	6.7324553	7
CTCCA	846555	1.6594452	5.6893263	1
CATGG	768760	1.6510793	14.513777	4
TGGGC	634795	1.6149908	5.3040805	5
AGGTG	698120	1.5694302	5.2620115	7
CCAGA	732250	1.5382624	5.2681403	3
TCAGG	697960	1.4990209	5.502503	3
CTTGG	702670	1.4740086	5.2082973	3
GGGCT	573205	1.4582989	5.3059454	6
CCTGA	709665	1.4561156	5.1018825	3
ACAGA	764495	1.4191259	5.622734	8
GGGTG	530725	1.4133211	5.2219896	7
AGGGC	532315	1.3865446	5.1001506	5
GGGGG	428100	1.3826253	6.039635	7
ACAGC	657440	1.3811063	5.8164625	8
ATGGG	614215	1.3808051	13.80031	5
GTCTT	789085	1.3648484	5.844504	1
GTGTG	614105	1.3484223	6.122561	1
TCTTG	761295	1.3167812	5.2045593	2
AACAG	702880	1.3047507	5.415897	7
ACTGG	605745	1.3009691	5.245938	8
CACAC	634495	1.273399	5.086038	5
ACATG	678915	1.2309294	11.955907	3
ACTGC	593625	1.2180207	5.032796	8
ACTGT	685685	1.214266	6.6401176	8
GTGCT	568730	1.1930392	5.7952237	1
AGCAC	559250	1.1748353	5.4623075	5
TACAG	632535	1.1468385	5.3278413	2
GGACA	514410	1.1311368	5.6933823	6
AGGAC	507490	1.1159204	6.202757	5
AGAGT	577100	1.0952255	5.97808	5
GACTG	501905	1.0779502	5.73558	7
ACAGT	590015	1.0697463	6.4395046	8
ATCAA	685570	1.0493252	7.7070036	3
GTCCT	518880	1.0398742	6.1909766	1
GGACT	475145	1.0204772	5.1389127	6
GTGCA	474150	1.0183402	6.219259	1
GTCCA	496035	1.0177821	6.6358256	1
TGGAC	471620	1.0129066	5.611239	5
TCAAC	559855	0.9697473	8.3434	4
GAGTA	501845	0.9524059	10.26252	1
GTACA	495210	0.8978569	16.070776	1
TACTG	503110	0.8909475	5.3705115	7
AGTAC	474650	0.86057997	7.4701557	2
TACAT	560635	0.8381272	10.323161	2
CGCAG	321905	0.8010473	11.187792	8
TATCA	511805	0.76512825	8.159565	2
GTATT	476425	0.7281661	5.0789995	1
GTGTA	376790	0.69843054	6.694242	1
GGTAT	330480	0.61258876	5.7957683	1
GTATC	312320	0.55308133	9.470641	1
GTATA	327895	0.51309675	6.0839787	1
ACGCA	226560	0.47594222	9.476969	7
CAACG	222990	0.4684426	9.447774	5
AACGC	190430	0.4000427	9.440074	6
>>END_MODULE