FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_UMI_03072016_R2.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_UMI_03072016_R2.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51776
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNN7481.4446847960444993No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNN5921.1433868974042027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNN4320.8343634116192831No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4250.8208436341161928No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNN3380.6528121137206429No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNN3100.5987330037082819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNNNNNN2380.459672435105068No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNNNNNNNN2190.4229758961681088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNNNNNNNNN2100.405593325092707No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNN1970.3804851668726823No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNN1720.3322002472187886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNN1710.3302688504326329No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANNNNNNNNN1530.2955037082818294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNNNNNNNN1530.2955037082818294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNNNNN1480.2858467243510507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNNNNNNNNN1320.25494437577255874No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNN1260.24335599505562422No Hit
NNTNNNNNNNNNNNNNNNNNNTNTTNTNNNNNNNNNNNNNNNNNNNNNNN1210.2336990111248455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNN1210.2336990111248455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNNNNNNNNN990.19120828182941904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNNNNNNNNN900.17382571075401732No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNCNNNNNNNNNN820.15837453646477131No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNTNNNNNNNNNN750.14485475896168107No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNCNNNNNNNNNN740.14292336217552534No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNCNNNNNNNNNN730.1409919653893696No Hit
NNGNNNNNNNNNNNNNNNNNNTNTTNTNNNNNNNNNNNNNNNNNNNNNNN730.1409919653893696No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNANNNNNNNNNN720.13906056860321384No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNTNNNNNNNNNN720.13906056860321384No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNCNNNNNNNNNN690.1332663782447466No Hit
NNTNNNNNNNNNNNNNNNNNNTNGTNTNNNNNNNNNNNNNNNNNNNNNNN660.12747218788627937No Hit
NNCNNNNNNNNNNNNNNNNNNTNTTNTNNNNNNNNNNNNNNNNNNNNNNN660.12747218788627937No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNCNNNNNNNNNN650.12554079110012362No Hit
NNTNNNNNNNNNNNNNNNNNNTNTTNGNNNNNNNNNNNNNNNNNNNNNNN600.11588380716934486No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGNNNNNNNNN590.11395241038318912No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNTNNNNNNNNNN590.11395241038318912No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNANNNNNNNNNN580.11202101359703337No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNGNNNNNNNNNN570.11008961681087763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNN570.11008961681087763No Hit
NNTNNNNNNNNNNNNNNNNNNGNTTNTNNNNNNNNNNNNNNNNNNNNNNN540.10429542645241038No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNNNNNNNN530.10236402966625464No Hit
NNTNNNNNNNNNNNNNNNNNNTNTGNTNNNNNNNNNNNNNNNNNNNNNNN530.10236402966625464No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNGNNNNNNNNNN520.10043263288009889No Hit
NNNCNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNNNNNNNN520.10043263288009889No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers