FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_UMI_03072016_R1.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_UMI_03072016_R1.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51776
Sequences flagged as poor quality0
Sequence length10-76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACC150.002223089669.918843
GAAGTGG200.006946563752.439136
TCTCCCA500.00718850727.96753559
CGAGACA1051.649895E-523.90683769
CACGAGA1350.002461843415.93789269
ACGAGAC1350.002461843415.93789268
CGAGCCC3000.00513139979.32251365
TCCGAGC3753.1919035E-49.32251259
CCGAGCC3450.00156216869.11984960
CTCCGAG4000.00570338137.904053767
ACACATC6552.4462523E-57.47946966
ACATCTC5309.0815587E-47.442695668
CACATCT5854.0331433E-47.20597567
ATACACA7652.5502808E-56.85478864
TACACAT6600.00168337356.35625865
TATACAC8507.404175E-45.758022363
TTATACA8250.00328456125.50875762
Produced by FastQC (version 0.11.5)