FastQCFastQC Report
Sun 29 Jan 2017
IH03.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameIH03.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102382552
Sequences flagged as poor quality0
Sequence length1-39
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAG1201460.1173500734773636TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG1317800.031.63648216
TCGTGTA1393050.031.29753515
GTCGTGT1435250.031.06160414
CGTCGTG1462950.030.8605613
GCGTCGT1472250.030.6337812
AGCGTCG1485200.030.42274711
GAGCGTC1504250.030.02363610
GTAGGGA1395650.029.81389419
TGTAGGG1409100.029.67063918
AGAGCGT1534150.029.5405759
GTGTAGG1428400.029.25771317
AAGAGCG1581250.028.8856548
AGATCGG3151500.028.665851
GAGAACG421800.028.6239931
GAACGGG420000.028.4976623
CGTCTGA1583300.028.4577717
ACGTCTG1624350.028.0880816
TAGGGAA1502950.027.81066720
CACGTCT1622050.027.80320715
ACACGTC1620300.027.71815914
Produced by FastQC (version 0.11.5)